Newt regeneration genes regulate Wingless signaling to restore patterning in Drosophila eye

iScience, Journal Year: 2021, Volume and Issue: 24(10), P. 103166 - 103166

Published: Sept. 24, 2021

Newts utilize their unique genes to restore missing parts by strategic regulation of conserved signaling pathways. Lack genetic tools poses challenges determine the function such genes. Therefore, we used Drosophila eye model demonstrate potential 5 newt (Notophthalmus viridescens) gene(s), viropana1-viropana5 (vna1-vna5), which were ectopically expressed in L2 mutant and GMR-hid, GMR-GAL4 eye. exhibits loss ventral half early head involution defective (hid) triggers cell-death during later development. Surprisingly, significantly photoreceptor cells both GMR>hid background upregulating cell-proliferation blocking cell-death, regulating evolutionarily Wingless (Wg)/Wnt pathway exhibit non-cell-autonomous rescues. Further, Wg/Wnt acts downstream Our data highlights that proteins can regulate pathways trigger a robust restoration with weak capability.

Language: Английский

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Quantifying innervation facilitated by deep learning in wound healing

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Oct. 6, 2023

Abstract The peripheral nerves (PNs) innervate the dermis and epidermis, are suggested to play an important role in wound healing. Several methods quantify skin innervation during healing have been reported. Those usually require multiple observers, complex labor-intensive, noise/background associated with immunohistochemistry (IHC) images could cause quantification errors/user bias. In this study, we employed state-of-the-art deep neural network, Denoising Convolutional Neural Network (DnCNN), perform pre-processing effectively reduce noise IHC images. Additionally, utilized automated image analysis tool, assisted by Matlab, accurately determine extent of various stages 8 mm is generated using a circular biopsy punch wild-type mouse. Skin samples were collected on days 3, 7, 10 15, sections from paraffin-embedded tissues stained against pan-neuronal marker- protein-gene-product 9.5 (PGP 9.5) antibody. On day 3 negligible nerve fibers present throughout few only lateral boundaries wound. 10, slight increase fiber density appeared, which significantly increased 15. Importantly, found positive correlation (R 2 = 0.926) between re-epithelization, suggesting association re-innervation re-epithelization. These results established quantitative time course healing, method offers novel useful tool facilitate other tissues.

Language: Английский

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Comparative transcriptomic analysis and structure prediction of novel Newt proteins

PLoS ONE, Journal Year: 2019, Volume and Issue: 14(8), P. e0220416 - e0220416

Published: Aug. 16, 2019

Notophthalmus viridescens (Red-spotted Newt) possess amazing capabilities to regenerate their organs and other tissues. Previously, using a de novo assembly of the newt transcriptome combined with proteomic validation, our group identified novel family five protein members expressed in adult tissues during regeneration viridescens. The presence putative signal peptide suggests that all these proteins are secretory nature. Here we employed iterative threading refinement (I-TASSER) server generate three-dimensional structure Newt predicted function. Our data could act as ion transporters, be involved redox reaction(s). Due absence transgenic approaches N. viridescens, conservation genetic machinery across species, generated Drosophila melanogaster misexpress genes. Expression 2775 transcripts were compared between newly We found genes developmental process, cell cycle, apoptosis, immune response among those highly enriched. To validate RNA Seq. data, expression six regulated verified real time Quantitative Polymerase Chain Reaction (RT-qPCR). These graded gene patterns provide insight into function Newt, layout map for future studies field.

Language: Английский

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Zebra-Fishing for Regenerative Awakening in Mammals

Biomedicines, Journal Year: 2021, Volume and Issue: 9(1), P. 65 - 65

Published: Jan. 12, 2021

Regeneration is defined as the ability to regrow an organ or a tissue destroyed by degeneration injury. Many human degenerative diseases and pathologies, currently incurable, could be cured if functional tissues cells restored. Unfortunately, humans more generally mammals have limited regenerative capabilities, capacities that are even further declining with age, contrary simpler organisms. Initially thought lost during evolution, several studies revealed mechanisms still present in but latent thus they stimulated. To do so there pressing need identify fundamental of regeneration species able efficiently regenerate. Thanks its regenerate most organs tissues, zebrafish has become powerful model organism biology recently engendered number attesting validity awakening potential mammals. In this review we highlight studies, particularly liver, pancreas, retina, heart, brain spinal cord, which identified conserved molecular events proved beneficial restore murine helped clarify real clinical translation research

Language: Английский

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Deciphering regeneration through non-model animals: A century of experiments on cephalopod mollusks and an outlook at the future

Frontiers in Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 10

Published: Jan. 9, 2023

The advent of marine stations in the last quarter 19th Century has given biologists possibility observing and experimenting upon myriad organisms. Among them, cephalopod mollusks have attracted great attention from onset, thanks to their remarkable adaptability captivity a number biologically unique features including sophisticate behavioral repertoire, body patterning capacities under direct neural control complexity nervous system rivalling vertebrates. Surprisingly, capacity regenerate tissues complex structures, such as appendages, albeit been known for centuries, understudied over decades. Here, we will first review limited number, but fundamental studies on subject published between 1920 1970 discuss what they added our knowledge regeneration biological phenomenon. We also speculate how these relate epistemic disciplinary context, setting base study taxon. then frame peripherality cephalopods relation with experimental accessibility, comparison, established models, either simpler (such planarians), or more promising terms translation (urodeles). Last, explore potential growing relevance prospective models today, light novel opportunities provided by technological methodological advances, reconsider old problems new ones. recent development cutting-edge technologies made available cephalopods, like genome editing, is allowing important findings opening way toward avenues. contribution offered increase regenerative mechanisms through cross-species comparison lead better understanding cellular molecular machinery involved, shedding common pathways strategies different taxa evolved promote organs. Through dialogue biological/experimental historical/contextual perspectives, this article stimulate discussion around changing relations availability animal specificity, technical developments scientific trends contemporary biology medicine.

Language: Английский

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