Effect of oxyresveratrol under in vitro lipopolysaccharide-induced periodontitis environment DOI Creative Commons
Ju Ri Ye, Seung Hwan Park, Sang Wook Kang

et al.

BMC Oral Health, Journal Year: 2024, Volume and Issue: 24(1)

Published: Nov. 15, 2024

Abstract Background Oxyresveratrol is the main constituent of mulberries and has many bioactive properties beneficial to human health. The purpose this study was assess anti-inflammatory effects oxyresveratrol on in vitro periodontitis model. Methods Human periodontal ligament cells were treated with (0, 10, 20 µg/mL) for 72 h. Cell viability flow cytometry assays performed. To investigate effect inflammation, nitric oxide production under lipopolysaccharide stimulation assessed. Next, expression biomarkers associated inflammation evaluated. Scratch wound assay performed evaluate cell migration/proliferation potential stimulation. Results Periodontal toxicity not observed treatment. treatment significantly inhibited reduced MMP-2, MMP-9, TNF-α, IL-6, IL-8 expressions after Regarding migration/proliferation, open area (33.28 ± 6.80%) lowest ( p < 0.05). Conclusions Within limits study, lipopolysaccharide-induced promoted migration/proliferation. These findings suggest that could be valuable management diseases.

Language: Английский

Effect of oxyresveratrol under in vitro lipopolysaccharide-induced periodontitis environment DOI Creative Commons
Ju Ri Ye, Seung Hwan Park, Sang Wook Kang

et al.

BMC Oral Health, Journal Year: 2024, Volume and Issue: 24(1)

Published: Nov. 15, 2024

Abstract Background Oxyresveratrol is the main constituent of mulberries and has many bioactive properties beneficial to human health. The purpose this study was assess anti-inflammatory effects oxyresveratrol on in vitro periodontitis model. Methods Human periodontal ligament cells were treated with (0, 10, 20 µg/mL) for 72 h. Cell viability flow cytometry assays performed. To investigate effect inflammation, nitric oxide production under lipopolysaccharide stimulation assessed. Next, expression biomarkers associated inflammation evaluated. Scratch wound assay performed evaluate cell migration/proliferation potential stimulation. Results Periodontal toxicity not observed treatment. treatment significantly inhibited reduced MMP-2, MMP-9, TNF-α, IL-6, IL-8 expressions after Regarding migration/proliferation, open area (33.28 ± 6.80%) lowest ( p < 0.05). Conclusions Within limits study, lipopolysaccharide-induced promoted migration/proliferation. These findings suggest that could be valuable management diseases.

Language: Английский

Citations

0