A study on the functional role of the DHCR24 gene in gonadal differentiation and development of Macrobrachium nipponense DOI Creative Commons
Pengfei Cai,

Wenyi Zhang,

Sufei Jiang

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 27, 2024

Sex differentiation in crustaceans is a complex process influenced by various factors, including the androgenic gland and sex-related genes. This study characterized role of Mn-DHCR24 gene oriental river prawn (Macrobrachium nipponense). We used bioinformatics to analyze sequence features phylogenetic relationships single gene. The expression patterns across different tissues developmental stages were determined real-time PCR, its localization testis was situ hybridization. RNA interference knock down expression, followed examining changes sex ratio gonadal development at PL10 stage. Additionally, an enzyme-linked immunosorbent assay measured 17α-methyltestosterone levels, tissue sections characterize development. results indicated that high testis, which critical for sperm maturation differentiation. RNAi experiments showed during reproductive regulation rather than as master further regulated hormone-related genes, influencing steroid biosynthesis pathways. Together, these findings provided valuable insights into genetic hormonal mechanisms M. nipponense, supported monosex culture technology.

Language: Английский

Crustacean endocrinology DOI
Donald L. Mykles, Lisa Musgrove, Tomer Ventura

et al.

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Citations

0
Special Issue: Molecular Advance on Reproduction and Fertility of Aquatic Animals DOI Open Access
Hui Qiao, Sufei Jiang, Hongtuo Fu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11610 - 11610

Published: Oct. 29, 2024

Many commercial aquatic animals are cultured in a variety of countries and regions [...]

Language: Английский

Citations

0
A study on the functional role of the DHCR24 gene in gonadal differentiation and development of Macrobrachium nipponense DOI Creative Commons
Pengfei Cai,

Wenyi Zhang,

Sufei Jiang

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 27, 2024

Sex differentiation in crustaceans is a complex process influenced by various factors, including the androgenic gland and sex-related genes. This study characterized role of Mn-DHCR24 gene oriental river prawn (Macrobrachium nipponense). We used bioinformatics to analyze sequence features phylogenetic relationships single gene. The expression patterns across different tissues developmental stages were determined real-time PCR, its localization testis was situ hybridization. RNA interference knock down expression, followed examining changes sex ratio gonadal development at PL10 stage. Additionally, an enzyme-linked immunosorbent assay measured 17α-methyltestosterone levels, tissue sections characterize development. results indicated that high testis, which critical for sperm maturation differentiation. RNAi experiments showed during reproductive regulation rather than as master further regulated hormone-related genes, influencing steroid biosynthesis pathways. Together, these findings provided valuable insights into genetic hormonal mechanisms M. nipponense, supported monosex culture technology.

Language: Английский

Citations

0