Proceedings of the National Academy of Sciences,
Journal Year:
2025,
Volume and Issue:
122(11)
Published: March 10, 2025
Recurrent
implantation
failure
(RIF)
is
a
devastating
condition
that
leaves
many
undergoing
fertility
treatment
childless.
The
human
endometrium
receptive
to
blastocyst
for
brief
period,
the
window
of
implantation.
Critical
knowledge
underpinning
biological
processes
leading
RIF,
essential
effective
treatment,
lacking.
We
employed
spatial
transcriptomics
define
region-
and
cell-type-specific
differences
in
endometrial
gene
expression
luteinizing
hormone
timed
biopsies
between
women
with
RIF
(n
=
8)
fertile
controls
(FC)
8).
Differentially
expressed
genes
(DEGs)
were
identified
when
comparing
regions
FC
(685
luminal
epithelium,
293
glandular
419
subluminal
stroma,
264
functionalis
1,125
stromal
CD45
+
leukocytes,
1,049
CD56
leukocytes).
Only
57
DEGs
common
all
subregions
cell
types,
which
highlights
multiple
are
lost
examined
as
single
entity.
When
RIF-specific
leveraged
against
from
mouse
genetic
models,
associated
aberrant
embryo
phenotypes
observed,
mostly
immune
populations.
Dysregulated
pathways
specific
included
“WNT
signaling
pathway,”
altered
stroma.
“Response
estradiol”
“ovulation
cycle”
dysregulated
In
silico
drug
screening
potential
compounds
can
reverse
profile
(e.g.,
raloxifene,
bisoprolol).
Our
findings,
well-characterized
cohort,
highly
endorse
consideration
each
region
type
separate
entities.
Ignoring
individual
composite
populations
will
overlook
important
aberrations,
forego
targets,
lead
research
waste
pursuing
clinically
irrelevant
options.
Cardiovascular
diseases
constitute
a
marked
threat
to
global
health,
and
the
emergence
of
spatial
omics
technologies
has
revolutionized
cardiovascular
research.
This
review
explores
application
omics,
including
transcriptomics,
proteomics,
metabolomics,
genomics,
epigenomics,
providing
more
insight
into
molecular
cellular
foundations
disease
highlighting
critical
contributions
science,
discusses
future
prospects,
technological
advancements,
integration
multi-omics,
clinical
applications.
These
developments
should
contribute
understanding
guide
progress
precision
medicine,
targeted
therapies,
personalized
treatments.
iScience,
Journal Year:
2025,
Volume and Issue:
28(3), P. 112092 - 112092
Published: Feb. 22, 2025
The
evolving
field
of
neuroepigenetics
provides
important
insights
into
the
molecular
foundations
brain
function.
Novel
sequencing
technologies
have
identified
patient-specific
mutations
and
gene
expression
profiles
involved
in
shaping
epigenetic
landscape
during
neurodevelopment
disease.
Traditional
methods
to
investigate
consequences
chromatin-related
provide
valuable
phenotypic
but
often
lack
information
on
biochemical
mechanisms
underlying
these
processes.
Recent
studies,
however,
are
beginning
elucidate
how
structural
and/or
functional
aspects
histone,
DNA,
RNA
post-translational
modifications
affect
transcriptional
landscapes
neurological
phenotypes.
Here,
we
review
identification
regulators
from
genomic
studies
disease,
as
well
mechanistic
findings
that
reveal
intricacies
neuronal
chromatin
regulation.
We
then
discuss
serve
a
guideline
for
future
investigations.
end
by
proposing
roadmap
therapies
exploit
coupling
them
recent
advances
targeted
therapeutics.
Plant Biotechnology Journal,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 7, 2025
Regeneration
represents
a
fundamental
biological
process
wherein
an
organism's
tissues
or
organs
repair
and
replace
themselves
following
damage
environmental
stress.
In
plant
systems,
injured
tree
branches
can
regenerate
adventitious
buds
develop
new
crowns
through
propagation
techniques
like
cuttings
canopy
pruning,
while
transgenic
plants
emerge
via
tissue
culture
in
genetic
engineering
processes
intimately
connected
to
regeneration
mechanisms.
The
advancement
of
technology
is
critical
for
addressing
complex
dynamic
climate
challenges,
ultimately
ensuring
global
agricultural
sustainability.
This
review
comprehensively
synthesizes
the
latest
transformation
technologies,
including
systems
across
woody,
herbaceous
algal
species,
organellar
modifications,
crucial
factors
facilitating
Agrobacterium-mediated
transformations,
intricate
hormonal
networks
regulating
regeneration,
comparative
analyses
transient
approaches
marker
gene
dynamics
throughout
processes.
Ultimately,
offers
novel
perspectives
on
current
bottlenecks
proposes
future
research
trajectories.
Proceedings of the National Academy of Sciences,
Journal Year:
2025,
Volume and Issue:
122(11)
Published: March 10, 2025
Recurrent
implantation
failure
(RIF)
is
a
devastating
condition
that
leaves
many
undergoing
fertility
treatment
childless.
The
human
endometrium
receptive
to
blastocyst
for
brief
period,
the
window
of
implantation.
Critical
knowledge
underpinning
biological
processes
leading
RIF,
essential
effective
treatment,
lacking.
We
employed
spatial
transcriptomics
define
region-
and
cell-type-specific
differences
in
endometrial
gene
expression
luteinizing
hormone
timed
biopsies
between
women
with
RIF
(n
=
8)
fertile
controls
(FC)
8).
Differentially
expressed
genes
(DEGs)
were
identified
when
comparing
regions
FC
(685
luminal
epithelium,
293
glandular
419
subluminal
stroma,
264
functionalis
1,125
stromal
CD45
+
leukocytes,
1,049
CD56
leukocytes).
Only
57
DEGs
common
all
subregions
cell
types,
which
highlights
multiple
are
lost
examined
as
single
entity.
When
RIF-specific
leveraged
against
from
mouse
genetic
models,
associated
aberrant
embryo
phenotypes
observed,
mostly
immune
populations.
Dysregulated
pathways
specific
included
“WNT
signaling
pathway,”
altered
stroma.
“Response
estradiol”
“ovulation
cycle”
dysregulated
In
silico
drug
screening
potential
compounds
can
reverse
profile
(e.g.,
raloxifene,
bisoprolol).
Our
findings,
well-characterized
cohort,
highly
endorse
consideration
each
region
type
separate
entities.
Ignoring
individual
composite
populations
will
overlook
important
aberrations,
forego
targets,
lead
research
waste
pursuing
clinically
irrelevant
options.
