Photoperiod Induces the Epigenetic Change of the GNAQ Gene in OVX+E2 Ewes DOI Open Access
Wei Wang,

Xiaolong Du,

Mingxing Chu

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(22), P. 16442 - 16442

Published: Nov. 17, 2023

GNAQ, a member of the alpha subunit encoding q-like G protein, is critical gene in cell signaling, and multiple studies have shown that upregulation GNAQ expression ultimately inhibits proliferation gonadotropin-releasing hormone (GnRH) neurons GnRH secretion, affects mammalian reproduction. Photoperiod key inducer which plays an important role regulation by affecting epigenetic modification. However, fewer confirmed how photoperiod induces modifications gene. In this study, we examined changes hypothalamus ovariectomized estradiol-treated (OVX+E2) sheep under three treatments (short treatment for 42 days, SP42; long LP42; days short followed photoperiod, SP-LP42). The results showed was significantly higher SP-LP42 than SP42 LP42 (p < 0.05). Whole genome methylation sequencing (WGBS) there are differentially methylated regions (DMRs) loci between different groups GNAQ. Among them, DNA level DMRs at CpG1 locus 0.01). Subsequently, core promoter region located with 1100 to 1500 bp upstream, all eight CpG sites those 0.01), except site 2 4 first fragment 0.05) region. acetylated histone H3 control group photoperiods 0.01); acetylation hypothalamic genomic protein lower This suggests binds gene, implying epigenetically regulated through acetylation. summary, suggest can induce hypothesize two may be factors regulating differential photoperiods, thus hypothalamus–pituitary–gonadal axis (HPGA) seasonal estrus sheep. study will provide some new information understand function reproduction

Language: Английский

Photoperiod Induces the Epigenetic Change of the GNAQ Gene in OVX+E2 Ewes DOI Open Access
Wei Wang,

Xiaolong Du,

Mingxing Chu

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(22), P. 16442 - 16442

Published: Nov. 17, 2023

GNAQ, a member of the alpha subunit encoding q-like G protein, is critical gene in cell signaling, and multiple studies have shown that upregulation GNAQ expression ultimately inhibits proliferation gonadotropin-releasing hormone (GnRH) neurons GnRH secretion, affects mammalian reproduction. Photoperiod key inducer which plays an important role regulation by affecting epigenetic modification. However, fewer confirmed how photoperiod induces modifications gene. In this study, we examined changes hypothalamus ovariectomized estradiol-treated (OVX+E2) sheep under three treatments (short treatment for 42 days, SP42; long LP42; days short followed photoperiod, SP-LP42). The results showed was significantly higher SP-LP42 than SP42 LP42 (p < 0.05). Whole genome methylation sequencing (WGBS) there are differentially methylated regions (DMRs) loci between different groups GNAQ. Among them, DNA level DMRs at CpG1 locus 0.01). Subsequently, core promoter region located with 1100 to 1500 bp upstream, all eight CpG sites those 0.01), except site 2 4 first fragment 0.05) region. acetylated histone H3 control group photoperiods 0.01); acetylation hypothalamic genomic protein lower This suggests binds gene, implying epigenetically regulated through acetylation. summary, suggest can induce hypothesize two may be factors regulating differential photoperiods, thus hypothalamus–pituitary–gonadal axis (HPGA) seasonal estrus sheep. study will provide some new information understand function reproduction

Language: Английский

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