Single-nuclei transcriptomes in the hypothalamus and POA of a highly social cichlid DOI Open Access

Isaac Miller-Crews,

Hans A. Hofmann

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 7, 2024

ABSTRACT How does complex behavior arise from the genome across biological levels? Specific cell types have evolutionarily conserved functional roles in regulating and maintaining various aspects of social behavior. There is a long history research demonstrating how specific cells hypothalamus POA are critical status reproductive This function dependent not just on one gene, but entire gene expression network working within each cell. The brain heterogenous tissue made up broad diversity cells. Targeting cellular-level transcriptomes socially relevant regions enables identification mediators. Single-nuclei RNA-sequencing (snRNA-seq) provides resolution to identify cell-specific transcriptomes, with more practical benefits compared single-cell approaches. Single-nucleus sequencing neuronal has remained fairly limited model mammalian organisms. Here we performed snRNA-seq Astatotilapia burtoni , fish system ideal for neuroscience. Male A. characterized by plastic phenotypes associated status, dominant subordinate, well-established differences neural patterns neuron morphology. novel opportunity apply non-model using hypothesis driven We show changes linked distinct transcriptomic profiles at cellular level.

Language: Английский

Dominance and aggressiveness are associated with vasotocin neuron numbers in a cooperatively breeding cichlid fish DOI Creative Commons
Tommaso Ruberto, William T. Swaney, Adam R. Reddon

et al.

Hormones and Behavior, Journal Year: 2025, Volume and Issue: 168, P. 105677 - 105677

Published: Jan. 20, 2025

Within dominance hierarchies, individuals must interact in a rank-appropriate manner, thus behavior and its underlying neural mechanisms change with social status. One such potential mechanism is arginine vasotocin (AVT), nonapeptide which has been implicated the regulation of aggression across vertebrate taxa. We investigated relationship between status, dominance-related behaviors, neuron counts daffodil cichlids (Neolamprologus pulcher). Daffodil live stable, mixed-sex, cooperatively breeding groups that are organised into linear hierarchies. Group members both sexes exhibit complex behavioral repertoires differ depending on their current recorded agonistic behaviors within correlated these number AVT cells three distinct neuronal populations preoptic area brain, comparing status sex. found parvocellular neurons were more abundant dominant than subordinates. also numbers magnocellular positively associated individuals. unrelated to submissive subordinate fish. Our data emphasise role modulating vertebrates.

Language: Английский

Citations

0
Tracking spatial patterns and daily modulation of behavior in a natural population of the pulse-type weakly electric fish, Gymnotus omarorum DOI Creative Commons
Adriana Migliaro, Federico Pedraja, Stefan Mucha

et al.

iScience, Journal Year: 2025, Volume and Issue: 28(3), P. 112018 - 112018

Published: Feb. 13, 2025

Tracking individual spatial and activity-rest patterns in natural populations is challenging because it seldom possible to monitor individual-specific traits continuously. The continuous emission of electric signals (EODs) by weakly fish provides a unique opportunity do this. We present cutting-edge technique, arrays electrodes connected low-cost amplifiers tracking algorithm, provide the identification pulse-type wild. Based only on EOD recordings individuals Gymnotus omarorum, we show that (1) there are more core than edge zones; (2) transitions into out recording sites were frequent at night, (3) resident robust nocturnal increases rate likely associated with daily variations water temperature. This experimental approach can be extended other species improve our understanding behavior, ecology, well-being environments.

Language: Английский

Citations

0
Single-nuclei transcriptomes in the hypothalamus and POA of a highly social cichlid DOI Open Access

Isaac Miller-Crews,

Hans A. Hofmann

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 7, 2024

ABSTRACT How does complex behavior arise from the genome across biological levels? Specific cell types have evolutionarily conserved functional roles in regulating and maintaining various aspects of social behavior. There is a long history research demonstrating how specific cells hypothalamus POA are critical status reproductive This function dependent not just on one gene, but entire gene expression network working within each cell. The brain heterogenous tissue made up broad diversity cells. Targeting cellular-level transcriptomes socially relevant regions enables identification mediators. Single-nuclei RNA-sequencing (snRNA-seq) provides resolution to identify cell-specific transcriptomes, with more practical benefits compared single-cell approaches. Single-nucleus sequencing neuronal has remained fairly limited model mammalian organisms. Here we performed snRNA-seq Astatotilapia burtoni , fish system ideal for neuroscience. Male A. characterized by plastic phenotypes associated status, dominant subordinate, well-established differences neural patterns neuron morphology. novel opportunity apply non-model using hypothesis driven We show changes linked distinct transcriptomic profiles at cellular level.

Language: Английский

Citations

0