Cardiometabolic Index is associated with heart failure: a cross-sectional study based on NHANES DOI Creative Commons
Zhu Xiao-ming, Yan Xu, Jie Zhang

et al.

Frontiers in Medicine, Journal Year: 2024, Volume and Issue: 11

Published: Dec. 9, 2024

Heart failure is a complex syndrome characterized by impaired cardiac function. Despite improvements in treatment, the prevalence of heart continues to rise. The Cardiometabolic Index (CMI), novel measure combining abdominal obesity and lipid levels, has emerged as potential predictor metabolic risk.

Language: Английский

Molecular Regulation of Cardiomyocyte Maturation DOI
Bhavana Shewale,

Tasneem Ebrahim,

Arushi Samal

et al.

Current Cardiology Reports, Journal Year: 2025, Volume and Issue: 27(1)

Published: Jan. 21, 2025

Language: Английский

Citations

3
Molecular and cellular neurocardiology in heart disease DOI
Beth A. Habecker, Donald M. Bers, Susan J. Birren

et al.

The Journal of Physiology, Journal Year: 2024, Volume and Issue: unknown

Published: May 22, 2024

Abstract This paper updates and builds on a previous White Paper in this journal that some of us contributed to concerning the molecular cellular basis cardiac neurobiology heart disease. Here we focus recent findings underpin autonomic development, novel intracellular pathways neuroplasticity. Throughout highlight unanswered questions areas controversy. Whilst neurochemical are already demonstrating prognostic viability patients with failure, also discuss opportunity better understand sympathetic impairment by using patient specific stem cells provides pathophysiological contextualization study ‘disease dish’. Novel imaging techniques spatial transcriptomics facilitating road map for target discovery may form therapeutic treat dysautonomia. image

Language: Английский

Citations

9
Cardiac Regeneration in Adult Zebrafish: A Review of Signaling and Metabolic Coordination DOI
Arkadeep Mitra, Subhadeep Mandal,

Kalyan Banerjee

et al.

Current Cardiology Reports, Journal Year: 2025, Volume and Issue: 27(1)

Published: Jan. 10, 2025

Language: Английский

Citations

0
GDF10 promotes rodent cardiomyocyte maturation during the postnatal period DOI
Maria Uscategui Calderon,

Maria L Spaeth,

Marissa Granitto

et al.

Journal of Molecular and Cellular Cardiology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
A Platform Integrating Biophysical and Biochemical Stimuli to Enhance Differentiation and Maturation of Cardiomyocyte Subtypes Derived from Human Induced Pluripotent Stem Cells DOI Creative Commons
Zhonggang Feng,

K Sawada,

Iori Ando

et al.

Journal of Cardiovascular Development and Disease, Journal Year: 2025, Volume and Issue: 12(2), P. 56 - 56

Published: Feb. 4, 2025

To enhance the differentiation and maturation of cardiomyocytes derived from human induced pluripotent stem cells, we developed a bioreactor system that simultaneously imposes biophysical biochemical stimuli on these committed cardiomyocytes. The cells were cultured within biohydrogels composed extracellular matrix extracted goat ventricles purchased rat-origin collagen, which housed in elastic PDMS culture chambers bioreactor. Elastic flexible electrodes PEDOT/PSS, latex, graphene flakes embedded hydrogels chamber walls, allowing cyclic stretch electrical pulses to be coordinately applied cells. Furthermore, dynamic analysis method employing transverse forced oscillation theory cantilever was used analyze discriminate subtype-specific beating behavior It found myosin light chain 2v (MLC2v), ventricular cell marker, primarily upregulated aggregated (+) electrode side, while with faint MLC2v but strong cardiac troponin T (cTNT) expression at ground (GND) side. mRNA using rtPCR gel dynamics further suggested subtype deviation different sides. This study demonstrated potential our enhancing maturation, it showed an intriguing phenomenon cardiomyocyte aggregation electrodes, may into new separation subtypes.

Language: Английский

Citations

0
Clinical and Genetic Heterogeneity of HCM: The Possible Role of a Deletion Involving MYH6 and MYH7 DOI Open Access

Giancarlo Mancuso,

Marina Marsan,

Paola Neroni

et al.

Genes, Journal Year: 2025, Volume and Issue: 16(2), P. 212 - 212

Published: Feb. 10, 2025

Background/Objectives: Pediatric hypertrophic cardiomyopathy (HCM) is the most common genetic myocardial disorder in children and a leading cause of sudden cardiac death (SCD) among young. Its phenotypic variability, driven by incomplete penetrance variable expressivity, presents significant challenges diagnosis clinical management. Methods: In this study, we report unique case 16-month-old female diagnosed with HCM caused rare deletion. Molecular analysis was performed using multigene panel chromosomal microarray (CMA). Results: tests identified 30 kb deletion encompassing MYH6 MYH7 genes. These genes are critical components sarcomeric architecture, known associations to other cardiomyopathies. Conclusions: This underscores heterogeneity HCM, highlighting importance considering genomic deletions involving key diagnostic evaluation.

Language: Английский

Citations

0
Charting Postnatal Heart Development Using In Vivo Single-Cell Functional Genomics DOI Open Access
Haofei Wang, Yanhan Dong,

Y.H. Song

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

The transition at birth, marked by increased circulatory demands and rapid growth, necessitates extensive remodeling of the heart's structure, function, metabolism. This transformation requires precise spatial temporal coordination among diverse cardiac cell types; central to this process is cardiomyocyte maturation, yet regulatory mechanisms driving these changes remain poorly understood. Here, we present a atlas postnatal hearts integrating single-nucleus transcriptomics with image-based transcriptomics, which uncovers dynamic networks maturation. To functionally interrogate candidate regulators in vivo , developed Probe-based Indel-detectable Perturb-seq (PIP-seq), high-throughput platform that uses probe-based chemistry directly capture sgRNA expression, perturbation status, transcriptomic profiles resolution. Applying PIP-seq development identified 21 novel highlighting critical nodal points process. Our study establishes high-resolution framework for dissecting heart development, underscoring integrative highly ordered roles microenvironment intercellular communication Importantly, enables systematic, exploration gene function underlying complex biological processes their native context.

Language: Английский

Citations

0
Comprehensive promotion of iPSC-CM maturation by integrating metabolic medium with nanopatterning and electrostimulation DOI Creative Commons
Wener Li, Xiaojing Luo,

Anna Strano

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 21, 2025

Abstract The immaturity of human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) is a major limitation for their use in drug screening to identify pro-arrhythmogenic or cardiotoxic molecules. Here, we demonstrate an approach that combines lipid-enriched maturation medium with high concentration calcium, nanopatterning culture surfaces and electrostimulation generate iPSC-CMs advanced electrophysiological, structural metabolic phenotypes. Systematic testing reveals the key driver enhanced mitochondrial development improved electrophysiological properties iPSC-CMs. Increased calcium strongly promotes maturation, while primarily facilitates sarcomere organisation minor effect on properties. Transcriptome analysis activation HMCES TFAM targets contributes development, whereas downregulation MAPK/PI3K SRF associated iPSC-CM polyploidy. These findings provide mechanistic insights into paving way pharmacological responses more closely resemble those adult CMs.

Language: Английский

Citations

0
Current and future diagnostics of congenital heart disease (CHD) DOI
Marc‐Phillip Hitz, Gregor Dombrowsky,

Н А Мельник

et al.

Medizinische Genetik, Journal Year: 2025, Volume and Issue: 37(2), P. 95 - 102

Published: April 4, 2025

Language: Английский

Citations

0
Pathobiology of myocardial and cardiomyocyte injury in ischemic heart disease: Perspective from seventy years of cell injury research DOI Creative Commons
L. Maximilian Buja

Experimental and Molecular Pathology, Journal Year: 2024, Volume and Issue: 140, P. 104944 - 104944

Published: Nov. 21, 2024

Language: Английский

Citations

3