Biotechnology Journal,
Journal Year:
2019,
Volume and Issue:
14(9)
Published: March 30, 2019
The
chemical
industry
has
made
a
contribution
to
modern
society
by
providing
cost‐competitive
products
for
our
daily
use.
However,
it
now
faces
serious
challenge
regarding
environmental
pollutions
and
greenhouse
gas
emission.
With
the
rapid
development
of
molecular
biology,
biochemistry,
synthetic
industrial
biotechnology
evolved
become
more
efficient
production
chemicals
materials.
in
contrast
industries,
current
(CIB)
is
still
not
competitive
chemicals,
materials,
biofuels
due
their
low
efficiency
complicated
sterilization
processes
as
well
high‐energy
consumption.
It
must
be
further
developed
into
“next‐generation
biotechnology”
(NGIB),
which
low‐cost
mixed
substrates
based
on
less
freshwater
consumption,
energy‐saving,
long‐lasting
open
continuous
intelligent
processing,
overcoming
shortcomings
CIB
transforming
processes.
Contamination‐resistant
microorganism
chassis
key
successful
NGIB,
requires
resistance
microbial
or
phage
contaminations,
available
tools
methods
metabolic
biology
engineering.
This
review
proposes
list
contamination‐resistant
bacteria
takes
Halomonas
spp.
an
example
variety
products,
including
polyhydroxyalkanoates
under
open‐
continuous‐processing
conditions
proposed
NGIB.
Metabolic Engineering Communications,
Journal Year:
2020,
Volume and Issue:
11, P. e00143 - e00143
Published: Aug. 29, 2020
KT2440
is
a
promising
bacterial
chassis
for
the
conversion
of
lignin-derived
aromatic
compound
mixtures
to
biofuels
and
bioproducts.
Despite
inherent
robustness
this
strain,
further
improvements
catabolism
toxicity
tolerance
Nature Communications,
Journal Year:
2020,
Volume and Issue:
11(1)
Published: Oct. 23, 2020
Abstract
High
titer,
rate,
yield
(TRY),
and
scalability
are
challenging
metrics
to
achieve
due
trade-offs
between
carbon
use
for
growth
production.
To
these
metrics,
we
take
the
minimal
cut
set
(MCS)
approach
that
predicts
metabolic
reactions
elimination
couple
metabolite
production
strongly
with
growth.
We
compute
MCS
solution-sets
a
non-native
product
indigoidine,
sustainable
pigment,
in
Pseudomonas
putida
KT2440,
an
emerging
industrial
microbe.
From
63
solution-sets,
our
omics
guided
process
identifies
one
experimentally
feasible
solution
requiring
14
simultaneous
reaction
interventions.
implement
total
of
genes
knockdowns
using
multiplex-CRISPRi.
MCS-based
shifts
from
stationary
exponential
phase.
25.6
g/L,
0.22
g/l/h,
~50%
maximum
theoretical
(0.33
g
indigoidine/g
glucose).
These
phenotypes
maintained
batch
fed-batch
mode,
across
scales
(100-ml
shake
flasks,
250-ml
ambr®,
2-L
bioreactors).
Frontiers in Bioengineering and Biotechnology,
Journal Year:
2020,
Volume and Issue:
7
Published: Jan. 17, 2020
Pseudomonas
putida
KT2440
is
a
well-established
chassis
in
industrial
biotechnology.
To
increase
the
substrate
spectrum,
we
implemented
three
alternative
xylose
utilization
pathways,
namely
Isomerase,
Weimberg,
and
Dahms
pathways.
The
synthetic
operons
contain
genes
from
Escherichia
coli
taiwanensis.
For
isolating
pathway
P.
two
(PP_2836
PP_4283),
encoding
an
endogenous
enzyme
of
Weimberg
regulator
for
glycolaldehyde
degradation,
were
deleted.
Before
after
adaptive
laboratory
evolution,
these
strains
characterized
terms
growth
synthesis
mono-rhamnolipids
pyocyanin.
engineered
strain
using
reached
highest
maximal
rate
0.30
h-1.
After
evolution
lag
phase
was
reduced
significantly.
titers
720
mg
L-1
30
pyocyanin
by
evolved
or
Isomerase
pathway,
respectively.
different
stoichiometries
pathways
may
allow
engineering
tailored
valuable
bioproduct
synthesis.
Biotechnology Journal,
Journal Year:
2019,
Volume and Issue:
14(9)
Published: March 30, 2019
The
chemical
industry
has
made
a
contribution
to
modern
society
by
providing
cost‐competitive
products
for
our
daily
use.
However,
it
now
faces
serious
challenge
regarding
environmental
pollutions
and
greenhouse
gas
emission.
With
the
rapid
development
of
molecular
biology,
biochemistry,
synthetic
industrial
biotechnology
evolved
become
more
efficient
production
chemicals
materials.
in
contrast
industries,
current
(CIB)
is
still
not
competitive
chemicals,
materials,
biofuels
due
their
low
efficiency
complicated
sterilization
processes
as
well
high‐energy
consumption.
It
must
be
further
developed
into
“next‐generation
biotechnology”
(NGIB),
which
low‐cost
mixed
substrates
based
on
less
freshwater
consumption,
energy‐saving,
long‐lasting
open
continuous
intelligent
processing,
overcoming
shortcomings
CIB
transforming
processes.
Contamination‐resistant
microorganism
chassis
key
successful
NGIB,
requires
resistance
microbial
or
phage
contaminations,
available
tools
methods
metabolic
biology
engineering.
This
review
proposes
list
contamination‐resistant
bacteria
takes
Halomonas
spp.
an
example
variety
products,
including
polyhydroxyalkanoates
under
open‐
continuous‐processing
conditions
proposed
NGIB.
