Metabolic Engineering, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 1, 2024
Language: Английский
Current Opinion in Biotechnology, Journal Year: 2025, Volume and Issue: 93, P. 103308 - 103308
Published: May 5, 2025
Language: Английский
Citations
0
ACS Synthetic Biology, Journal Year: 2024, Volume and Issue: 13(7), P. 2177 - 2187
Published: July 5, 2024
Transcription factor (TF)-based biosensors have arisen as powerful tools in the advancement of metabolic engineering. However, with emergence numerous bioproduction targets, variety applicable TF-based remains severely limited. In this study, we investigated and engineered an 1,2-propanediol (1,2-PD)-responsive transcription activator, PocR, from Salmonella typhimurium to enrich current biosensor repertoire. Heterologous characterization PocR E. coli revealed a significantly limited operational range dynamic range, primarily attributed leaky binding between its corresponding promoters absence 1,2-PD inducer. Promiscuity uncovered minor responsiveness toward glycerol 1,2-butanediol (1,2-BD). Using AlphaFold-predicted structure protein mutagenesis, preliminarily explored underlying mechanism PocR. Based on mechanism, PcoR-F46R/G105D variant altered inducer specificity glycerol, well PocR-ARE (Q107A/S192R/A203E) nearly 4-fold higher (6.7-fold activation) 20-fold wider (0-20 mM 1,2-PD). Finally, successfully converted repressor through promoter Integrating activation repression functions established versatile 1,2-PD-induced bifunctional regulation system based PocR-ARE. Our work showcases exploration exploitation underexplored type transcriptional activator capable recruiting RNA polymerase. It also expands toolbox by providing 1,2-PD-responsive regulator glycerol-responsive activator.
Language: Английский
Citations
3
Biosensors, Journal Year: 2023, Volume and Issue: 13(6), P. 633 - 633
Published: June 7, 2023
Plant natural products (PNPs) have shown various pharmaceutical activities, possessing great potential in global markets. Microbial cell factories (MCFs) provide an economical and sustainable alternative for the synthesis of valuable PNPs compared with traditional approaches. However, heterologous synthetic pathways always lack native regulatory systems, bringing extra burden to production. To overcome challenges, biosensors been exploited engineered as powerful tools establishing artificial networks control enzyme expression response environments. Here, we reviewed recent progress involved application that are responsive their precursors. Specifically, key roles these played PNP pathways, including isoprenoids, flavonoids, stilbenoids alkaloids, were discussed detail.
Language: Английский
Citations
7
Synthetic biology and engineering, Journal Year: 2023, Volume and Issue: 1(2), P. 1 - 14
Published: Jan. 1, 2023
Establishing microbial cell factories has become a sustainable and increasingly promising approach for the synthesis of valuable chemicals. However, introducing heterologous pathways into these can disrupt endogenous cellular metabolism, leading to suboptimal production performance. To address this challenge, dynamic pathway regulation been developed proven effective in improving biosynthesis. In review, we summarized typical strategies based on their control logic. The applicable scenarios each logic were highlighted perspectives future research direction area discussed.
Language: Английский
Citations
4
ACS Synthetic Biology, Journal Year: 2023, Volume and Issue: 12(12), P. 3730 - 3742
Published: Nov. 30, 2023
Genetic logic gates can be employed in metabolic engineering and synthetic biology to regulate gene expression based on diverse inputs. Design of tunable genetic with versatile dynamic performance is essential for expanding the usability these toolsets. Here, using p-coumaric acid biosensor system as a proof-of-concept, we initially investigated parameters influencing buffer (BUF) gates. Subsequently, integrating binding sequences from tetR or lacI regulation systems into constitutive promoter yielded AND Additionally, characterized antisense RNAs (asRNAs) single guide (sgRNAs) various repression efficiencies were combined BUF construct suite acid-triggered NOT Finally, designed bifunctional circuits that subjected orthogonality evaluation. The established this study serve valuable tools future applications biology.
Language: Английский
Citations
4
ACS Synthetic Biology, Journal Year: 2023, Volume and Issue: 12(8), P. 2382 - 2392
Published: July 27, 2023
Transcriptional factors-based biosensors are commonly used in metabolic engineering for inducible control of gene expression and related applications such as high-throughput screening dynamic pathway regulations. Mining novel transcriptional factors is essential expanding the usability these toolsets. Here, we report identification, characterization, a phenolic acid responsive regulator PadR from Bacillus amyloliquefaciens (BaPadR). This BaPadR-based biosensor system showed unique ligand preference exhibited high output strength comparable to that systems. Through DNA binding region BaPadR, further enhanced range system. The sequences responsible BaPadR recognition were located by promoter truncation hybrid building. To explore tunability sensor system, base substitutions performed on decarboxylase (PpadC) promoter. can serve valuable tool future synthetic biology applications.
Language: Английский
Citations
3
Metabolic Engineering, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 1, 2024
Language: Английский
Citations
0
Metabolic Engineering, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 1, 2024
Language: Английский
Citations
0