Biotechnology for Biofuels and Bioproducts,
Journal Year:
2025,
Volume and Issue:
18(1)
Published: March 28, 2025
To
address
the
challenge
of
microbial
tolerance
in
industrial
biomanufacturing,
we
developed
an
adaptive
evolution
strategy
for
Escherichia
coli
W3110
to
enhance
its
salicylic
acid
(SA)
tolerance.
Utilizing
a
CmeR-PcmeO
biosensor-enabled
high-throughput
screening
system,
isolated
SA-tolerant
variant
(W3110K-4)
that
exhibited
2.3-fold
increase
(from
0.9
2.1
g/L)
and
2.1-fold
improvement
SA
production
283
588.1
mg/L).
Subsequently,
designed
sensors
were
combined
with
multi-pathway
sgRNA
arrays
dynamically
modulate
other
three
branched-chain
derivatives,
achieving
balance
between
biomass
growth
rapid
adaptively
evolved
strain,
resulting
maximum
yield
1477.8
mg/L,
which
represents
30%
over
non-evolved
control
strain
W3110K-W2
(1138.2
mg/L)
using
same
metabolic
strategy.
Whole-genome
sequencing
revealed
mutations
genes
such
as
ducA*
anti-drug
resistance
C2
mutation
(ymdA*,
ymdB*,
clsC*,
csgB*,
csgA*,
csgC*)
play
key
role
enhancing
productivity.
Notably,
W3110K-4
exhibits
significant
bacteriophages,
making
it
promising
candidate
large-scale
fermentation.
This
work
develops
expands
proposes
new
insights
into
improved
strains
through
biosensor
screening,
guided
metabolism,
evolution,
provides
paradigm
engineers
obtain
engineered
strains.
Metabolic Engineering,
Journal Year:
2024,
Volume and Issue:
85, P. 61 - 72
Published: July 20, 2024
Advances
in
synthetic
biology
and
artificial
intelligence
(AI)
have
provided
new
opportunities
for
modern
biotechnology.
High-performance
cell
factories,
the
backbone
of
industrial
biotechnology,
are
ultimately
responsible
determining
whether
a
bio-based
product
succeeds
or
fails
fierce
competition
with
petroleum-based
products.
To
date,
one
greatest
challenges
is
creation
high-performance
factories
consistent
efficient
manner.
As
so-called
white-box
models,
numerous
metabolic
network
models
been
developed
used
computational
strain
design.
Moreover,
great
progress
has
made
AI-powered
engineering
recent
years.
Both
approaches
advantages
disadvantages.
Therefore,
deep
integration
AI
crucial
construction
superior
higher
titres,
yields
production
rates.
The
detailed
applications
latest
advanced
design
summarized
this
review.
Additionally,
discussed.
It
anticipated
that
mechanistic
powered
by
will
pave
way
powerful
chassis
strains
coming
Biotechnology and Bioengineering,
Journal Year:
2024,
Volume and Issue:
121(7), P. 2147 - 2162
Published: April 26, 2024
P-coumaric
acid
(p-CA),
a
pant
metabolite
with
antioxidant
and
anti-inflammatory
activity,
is
extensively
utilized
in
biomedicine,
food,
cosmetics
industry.
In
this
study,
synthetic
pathway
(PAL)
for
p-CA
was
designed,
integrating
three
enzymes
(AtPAL2,
AtC4H,
AtATR2)
into
higher
l-phenylalanine-producing
strain
Escherichia
coli
PHE05.
However,
the
lower
soluble
expression
activity
of
AtC4H
PAL
bottleneck
increasing
titers.
To
overcome
limitation,
enhanced
through
N-terminal
modifications.
And
an
optimal
mutant,
Open Life Sciences,
Journal Year:
2024,
Volume and Issue:
19(1)
Published: Jan. 1, 2024
Abstract
Recent
advancements
in
protein/enzyme
engineering
have
enabled
the
production
of
a
diverse
array
high-value
compounds
microbial
systems
with
potential
for
industrial
applications.
The
goal
this
review
is
to
articulate
some
most
recent
protein
advances
bacteria,
yeast,
and
other
produce
valuable
substances.
These
substances
include
α-farnesene,
vitamin
B12,
fumaric
acid,
linalool,
glucaric
carminic
mycosporine-like
amino
acids,
patchoulol,
orcinol
glucoside,
d
-lactic
keratinase,
α-glucanotransferases,
β-glucosidase,
seleno-methylselenocysteine,
fatty
high-efficiency
β-glucosidase
enzymes,
cellulase,
β-carotene,
physcion,
glucoamylase.
Additionally,
enzyme
enhancing
thermostability
will
be
discussed.
findings
revolutionize
various
industries,
including
biotechnology,
food,
pharmaceuticals,
biofuels.
Journal of Agricultural and Food Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 12, 2025
Production
of
liquiritigenin,
a
plant-derived
significant
flavonoid
traditionally
extracted
from
licorice
plants,
is
constrained
by
ecological
and
operational
inefficiencies.
Despite
efforts
to
achieve
heterologous
reconstruction
liquiritigenin
synthesis
pathway
in
microorganisms,
the
titers
remain
low
process
still
at
proof-of-concept
stage,
insufficient
replace
plant
extraction.
Herein,
efficient
production
galactose
induction
system
Saccharomyces
cerevisiae
was
reengineered
for
better
decoupling
growth
stages,
making
it
more
suitable
pathways,
then
applied
naringenin-producing
strain
modified
redirect
production.
To
improve
ratio,
dual
NADPH
supply
developed
enhance
capabilities.
Subsequently,
concept
using
endogenous
metabolites
regulate
simplification
optimization
natural
product
biosynthetic
pathways
proposed,
general
metabolic
strategy
model
compounds,
aromatic
ester
model,
introduced.
The
final
engineered
achieved
867.67
mg/L
5
L
fermenter.
These
results
demonstrated
innovative
use
genetic
modifications
overcome
conventional
extraction
limitations,
providing
valuable
insights
synthesizing
flavonoids
other
products.
