Cited by Architecting a Transcriptional Repressor-based Genetic Inverter for Tryptophan Derived Pathway Regulation in Escherichia coli

Reprogramming Escherichia coli metabolism for butyrolactam production by the temperature-powered multi-genes control circuit DOI

Yang Li,

Changyang Yang,

Mingxiong Liu

et al.

Bioresource Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132438 - 132438

Published: March 1, 2025

Language: Английский

Citations

Engineering an Overflow‐Responsive Regulation System for Balancing Cellular Redox and Optimizing Microbial Production DOI

Jianli Zhang, Jian Wang, Tian Jiang

et al.

Biotechnology and Bioengineering, Journal Year: 2025, Volume and Issue: unknown

Published: March 21, 2025

ABSTRACT Escherichia coli accumulates acetate as a byproduct in fast growth aerobic conditions when using glucose carbon source. This phenomenon, known overflow metabolism, has negative impacts on cell and protein expression, also causes loss during biosynthesis most microbial production scenarios. In this study, we regarded the “waste” metabolite useful metabolism indicator, constructed an biosensor to monitor change of concentration converted signal into various regulation outputs. Phloroglucinol is phenolic compound with several derivatives that exhibit pharmacological activities. By applying bifunctional dynamic system phloroglucinol production, released cellular redox pressure real‐time reduced waste flux metabolism. Finally, was redirected more favorably towards desired product, resulting boosted titer 1.30 g/L, increased by 2.04‐fold. Overall, study explored use central byproduct‐responsive improving metabolic status, providing general approach for enhancing bioproduction.

Language: Английский

Citations

A rapid and efficient strategy for combinatorial repression of multiple genes in Escherichia coli DOI

Yi Zheng,

Yamada Mo,

Yingbo Yuan

et al.

Microbial Cell Factories, Journal Year: 2025, Volume and Issue: 24(1)

Published: March 28, 2025

Language: Английский

Citations

Investigating and Engineering an 1,2-Propanediol-Responsive Transcription Factor-Based Biosensor DOI

Yuxi Teng, Xinyu Gong,

Jianli Zhang

et al.

ACS Synthetic Biology, Journal Year: 2024, Volume and Issue: 13(7), P. 2177 - 2187

Published: July 5, 2024

Transcription factor (TF)-based biosensors have arisen as powerful tools in the advancement of metabolic engineering. However, with emergence numerous bioproduction targets, variety applicable TF-based remains severely limited. In this study, we investigated and engineered an 1,2-propanediol (1,2-PD)-responsive transcription activator, PocR, from Salmonella typhimurium to enrich current biosensor repertoire. Heterologous characterization PocR E. coli revealed a significantly limited operational range dynamic range, primarily attributed leaky binding between its corresponding promoters absence 1,2-PD inducer. Promiscuity uncovered minor responsiveness toward glycerol 1,2-butanediol (1,2-BD). Using AlphaFold-predicted structure protein mutagenesis, preliminarily explored underlying mechanism PocR. Based on mechanism, PcoR-F46R/G105D variant altered inducer specificity glycerol, well PocR-ARE (Q107A/S192R/A203E) nearly 4-fold higher (6.7-fold activation) 20-fold wider (0-20 mM 1,2-PD). Finally, successfully converted repressor through promoter Integrating activation repression functions established versatile 1,2-PD-induced bifunctional regulation system based PocR-ARE. Our work showcases exploration exploitation underexplored type transcriptional activator capable recruiting RNA polymerase. It also expands toolbox by providing 1,2-PD-responsive regulator glycerol-responsive activator.

Language: Английский

Citations

Architecting a Transcriptional Repressor-based Genetic Inverter for Tryptophan Derived Pathway Regulation in Escherichia coli DOI

Xinyu Gong, Yuxi Teng,

Jianli Zhang

et al.

Metabolic Engineering, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 1, 2024

Language: Английский

Citations