Lignin valorization to bioplastics with an aromatic hub metabolite-based autoregulation system DOI Creative Commons

Yiquan Zhao,

Le Xue, Zhiyi Huang

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Oct. 28, 2024

Exploring microorganisms with downstream synthetic advantages in lignin valorization is an effective strategy to increase target product diversity and yield. This study ingeniously engineers the non-lignin-degrading bacterium Ralstonia eutropha H16 (also known as Cupriavidus necator H16) convert lignin, a typically underutilized by-product of biorefinery, into valuable bioplastic polyhydroxybutyrate (PHB). The aromatic metabolism capacities R. for different lignin-derived aromatics (LDAs) are systematically characterized complemented by integrating robust functional modules including O-demethylation, aldehyde mitigation inhibition. A pivotal discovery regulatory element PcaQ, which highly responsive hub metabolite protocatechuic acid during degradation. Based on computer-aided design we develop metabolite-based autoregulation (HMA) system. system can control genes expression response heterologous LDAs enhance efficiency. Multi-module genome integration directed evolution further fortify strain's stability conversion capacities, leading PHB production titer 2.38 g/L using sole carbon source. work not only marks leap from components but also provides redesign non-LDAs-degrading microbes efficient valorization. One challenge that possessing metabolic unable lignin. Here, authors engineer efficiently self-enhanced

Language: Английский

Deciphering the mobility and bacterial hosts of antibiotic resistance genes under the coexistence of antibiotics and microplastics by metagenomic analysis and binning method DOI

Ming Xu,

Yuan Gao,

Yun-xiang Zhu

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 160029 - 160029

Published: Jan. 1, 2025

Language: Английский

Citations

0
Recent advances in enzymes active on lignin-derived aromatic compounds DOI
Megan E. Wolf, Lindsay D. Eltis

Trends in Biochemical Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
Co‐production of galactonic acid and lactobionic acid from cheese whey using recyclable engineered Pseudomonas putida DOI
Peng Liu,

Luo Hong,

Peng‐Cheng Fu

et al.

International Journal of Dairy Technology, Journal Year: 2025, Volume and Issue: 78(1)

Published: Feb. 1, 2025

Whey is a main by‐product generated during cheese manufacture. It contains abundant lactose and proteins valued raw material in many countries. Pseudomonas putida KT2440 promising microbial strain for biobased chemical production. In our previous studies, this was confirmed owning high ability to oxidise galactose. Besides, we have constructed recombinant P. (pBB‐GDH1) harbouring novel glucose dehydrogenase that capable of oxidising lactose. Based on these, the potential engineered whey utilisation deserves further exploration. This research aimed establish bioprocess convert into galactonic acid lactobionic using . Lactose powder hydrolysed by Bacillus coagulans β‐galactosidase inoculated hydrolysate galactose converted acid. Then, bacterial cells were recovered employed production from fresh solution. Three independent experiments performed, mean value taken as results. exhibited higher lactose‐oxidising activity compared with wild‐type strain. could utilise growth 25.45 g/L obtained productivity 1.06 g/L/h. The maintained excellent oxidation ability. After optimisation biocatalytic reactions, 301.58 containing 300 3 L bioreactor. reached 2.28 g/L/h yield 96.67%. study established sustainable economic aldonic acids co‐production strategy powder.

Language: Английский

Citations

0
Lignin valorization to bioplastics with an aromatic hub metabolite-based autoregulation system DOI Creative Commons

Yiquan Zhao,

Le Xue, Zhiyi Huang

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Oct. 28, 2024

Exploring microorganisms with downstream synthetic advantages in lignin valorization is an effective strategy to increase target product diversity and yield. This study ingeniously engineers the non-lignin-degrading bacterium Ralstonia eutropha H16 (also known as Cupriavidus necator H16) convert lignin, a typically underutilized by-product of biorefinery, into valuable bioplastic polyhydroxybutyrate (PHB). The aromatic metabolism capacities R. for different lignin-derived aromatics (LDAs) are systematically characterized complemented by integrating robust functional modules including O-demethylation, aldehyde mitigation inhibition. A pivotal discovery regulatory element PcaQ, which highly responsive hub metabolite protocatechuic acid during degradation. Based on computer-aided design we develop metabolite-based autoregulation (HMA) system. system can control genes expression response heterologous LDAs enhance efficiency. Multi-module genome integration directed evolution further fortify strain's stability conversion capacities, leading PHB production titer 2.38 g/L using sole carbon source. work not only marks leap from components but also provides redesign non-LDAs-degrading microbes efficient valorization. One challenge that possessing metabolic unable lignin. Here, authors engineer efficiently self-enhanced

Language: Английский

Citations

2