Highly Efficient In Vivo Production of Sialyllacto-N-tetraose C via Screening of Beneficial β1,4-galactosyltransferase and α2,6-sialyltransferase

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: 73(9), P. 5376 - 5384

Published: Feb. 18, 2025

Biological production of human milk oligosaccharides (HMOs) using metabolically engineered strains is a research hotspot in food biotechnology, but less effort has been made on the biological sialylated complex HMOs. Sialyllacto-N-tetraose c only monosialylated HMO top 15 In this study, metabolic pathway LST was constructed Escherichia coli BL21(DE3) by introducing three sequential glycosyltransferases: β1,3-N-acetylglucosaminyltransferase, β1,4-galactosyltransferase, and α2,6-sialyltransferase. The cytidine 5′-monophospho (CMP)-N-acetylneuraminic acid (Neu5Ac) enhanced to improve production. β1,4-galactosyltransferase from Helicobacter pylori J99 (HpGalT) α2,6-sialyltransferase Vespertiliibacter pulmonis (ED6ST) were screened as pair key glycosyltransferases for enhancing final strain could produce 1.718 9.745 g/L shake-flask fed-batch cultivation, respectively, indicating feasibility efficient biosynthesis

Language: Английский

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Design of a diblock-based membraneless organelle system for metabolic process control

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 160239 - 160239

Published: Feb. 1, 2025

Language: Английский

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Iterative multimodule reprogramming and protein engineering for efficient lactodifucotetraose bioproduction

Food Bioscience, Journal Year: 2024, Volume and Issue: unknown, P. 105364 - 105364

Published: Oct. 1, 2024

Language: Английский

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Engineering microbial cell factories by multiplexed spatiotemporal control of cellular metabolism: Advances, challenges, and future perspectives

Biotechnology Advances, Journal Year: 2024, Volume and Issue: 79, P. 108497 - 108497

Published: Dec. 5, 2024

Language: Английский

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