Reply on CC1 DOI Creative Commons
Evgeny Nimerovsky

Published: Dec. 20, 2024

Abstract. Enhanced transverse relaxation near rotary-resonance conditions is a well-documented effect for anisotropic solid samples undergoing magic-angle spinning (MAS). It surprising behavior rotating liquids, in which first-order interactions are averaged at much faster timescale as compared with the frequency. Here we report measurements of 13C under spin lock both polybutadiene rubber and polyethylene glycol solution. Maxima rates observed when spin-lock frequency matches one or two times MAS rate. Through simulations, qualitatively describe appearance this effect, can be explained by time dependence caused sample rotation an inhomogeneous rf-field distribution. Consideration important experiments based on conditions, motivates design new coils improved homogeneity.

Language: Английский

Nanosecond Molecular Motion in pHP1α Liquid–Liquid Phase Separation Captured by Solid-State NMR DOI

Sze Yuet Chin,

Lei Zhao,

Yinglu Chen

et al.

The Journal of Physical Chemistry Letters, Journal Year: 2025, Volume and Issue: unknown, P. 1150 - 1156

Published: Jan. 23, 2025

The relationship among protein structure, function, and dynamics is fundamental to biological activity, particularly in more complex biomolecular systems. Solid-state solution-state NMR techniques offer powerful means probe these across various time scales. However, standard assumptions about molecular motion are often challenged phase-separated systems like phosphorylated heterochromatin 1 alpha (pHP1α), which exhibit both solid- solution-like characteristics. This study investigates the nanosecond motions pHP1α liquid-liquid phase separation (LLPS) using relaxation hetNOE-filtered HSQC signals. By systematically analyzing captured by conventional HSQC, we characterize global site-specifically LLPS. Our findings reveal ∼15 ns LLPS system, suggesting coexistence of different dynamic phases, support previous observations on its role chromatin organization. work contributes expanding literature behavior, with implications for understanding basis compaction genomic stability.

Language: Английский

Citations

0
Special Issue Title: New Methods in Biomolecular Nuclear Magnetic Resonance Spectroscopy II DOI
T. Michael Sabo

Methods, Journal Year: 2024, Volume and Issue: 231, P. 57 - 60

Published: Sept. 10, 2024

Language: Английский

Citations

0
Response to all reviewer comments DOI Creative Commons
Evgeny Nimerovsky

Published: Dec. 20, 2024

Abstract. Enhanced transverse relaxation near rotary-resonance conditions is a well-documented effect for anisotropic solid samples undergoing magic-angle spinning (MAS). It surprising behavior rotating liquids, in which first-order interactions are averaged at much faster timescale as compared with the frequency. Here we report measurements of 13C under spin lock both polybutadiene rubber and polyethylene glycol solution. Maxima rates observed when spin-lock frequency matches one or two times MAS rate. Through simulations, qualitatively describe appearance this effect, can be explained by time dependence caused sample rotation an inhomogeneous rf-field distribution. Consideration important experiments based on conditions, motivates design new coils improved homogeneity.

Language: Английский

Citations

0
Reply on CC1 DOI Creative Commons
Evgeny Nimerovsky

Published: Dec. 20, 2024

Abstract. Enhanced transverse relaxation near rotary-resonance conditions is a well-documented effect for anisotropic solid samples undergoing magic-angle spinning (MAS). It surprising behavior rotating liquids, in which first-order interactions are averaged at much faster timescale as compared with the frequency. Here we report measurements of 13C under spin lock both polybutadiene rubber and polyethylene glycol solution. Maxima rates observed when spin-lock frequency matches one or two times MAS rate. Through simulations, qualitatively describe appearance this effect, can be explained by time dependence caused sample rotation an inhomogeneous rf-field distribution. Consideration important experiments based on conditions, motivates design new coils improved homogeneity.

Language: Английский

Citations

0