New entomopathogenic species in the Clavicipitaceae family (Hypocreales, Ascomycota) from the subtropical forests of Fujian, China DOI Creative Commons

Longbing Lin,

Yongsheng Lin,

Nemat O. Keyhani

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 3, 2025

Introduction Entomopathogenic fungi play a crucial role in the ecological regulation of insect populations and can be exploited as resource for pest control, sustainable agriculture, natural products discovery. These their infected hosts are sometimes highly coveted part traditional medicine practices. Here, we sought to examine biodiversity entomogenous subtropical forests China. Methods Fungal-infected specimens were collected from various sites Fujian Province, China, purified isolates obtained through laboratory cultivation isolation techniques. Molecular characterization specific target genomic loci was performed on fungal isolates, used phylogenetic analyses using Bayesian inference maximum likelihood methods elucidate taxonomic relationships. Microscopy describe morphological features isolates. Results Through comprehensive two-year survey Province via multilocus molecular analysis targeting nr SSU , LSU tef1-α rpb1, rpb2 specimens, identified three novel species within Clavicipitaceae herein described as: Albacillium fuzhouense sp. nov., Conoideocrella gongyashanensis nov. Neoaraneomyces wuyishanensis well recently recorded, Metarhizium cicadae . Each new also distinguished its closest relatives by unique characteristics. Discussion discoveries enrich our understanding family contribute development control strategies

Language: Английский

Molecular Phylogenetics and Estimation of Evolutionary Divergence and Biogeography of the Family Cordycipitaceae (Ascomycota, Hypocreales) DOI Creative Commons

Huili Pu,

Jiao Yang, Nemat O. Keyhani

et al.

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(1), P. 28 - 28

Published: Jan. 2, 2025

The Cordycipitaceae family of insecticidal fungi is widely distributed in nature, the most complex order Hypocreales (Ascomycota), with members displaying a diversity morphological characteristics and insect host ranges. Based on Bayesian evolutionary analysis five genomic loci(the small subunit ribosomal RNA (SSU) gene, large (LSU) translation elongation factor 1-α (tef1-α) largest polymerase II (rpb1), second (rpb2), we inferred divergence times for Cordycipitaceae, improving internal phylogeny this fungal family. Molecular clock analyses indicate that ancestor Akanthomyces sensu lato occurred Paleogene period (34.57 Mya, 95% HPD: 31.41–37.67 Mya), species appeared Neogene period. historical biogeography was reconstructed using reconstructing ancestral state phylogenies (RASP) analysis, indicating it likely originated Asia. Combined characterization phylogenetic were used to identify taxonomically place within Cordycipitaceae. These include following: (i) two new species, namely baishanensis sp. nov. Samsoniella sanmingense nov., (ii) record isolated from infected Lepidopteran host, Blackwellomyces lateris, (iii) genus Niveomyces, sporothrix-like asexual morphs, N. multisynnematus, dipteran insects (flies), (iv) known (hyper-) mycoparasite, Liangia sinensis, fungus Ophiocordyceps globiceps (Ophiocordycipitaceae) growing host. Our data provide significant addition diversity, ecology, aspects

Language: Английский

Citations

1
Novel genus and species of Diaporthostomataceae (Diaporthales) in China DOI Creative Commons
Ning Jiang, Han Xue, Yong Li

et al.

IMA Fungus, Journal Year: 2025, Volume and Issue: 16

Published: March 7, 2025

Diaporthales is a significant fungal order comprising species that predominantly inhabit plant tissues, being pathogens, endophytes, and saprobes. Recent studies have uncovered extensive diversity across various hosts, utilizing both morphological characteristics molecular phylogenetic analyses. In this study, samples of leaf spots branch cankers were collected from China, isolations established. Species identification was conducted using approach based on combined sequence data the internal transcribed spacer (ITS) region, large subunit ribosomal DNA (LSU), DNA-directed RNA polymerase II second largest (rpb2), translation elongation factor 1-alpha (tef1) genes, together with observations. As result, novel genus Tiania proposed, three newly described species: T.chinensis, T.lithocarpicola, T.quercicola. These are validated by pairwise homoplasy index (PHI) analysis, ensuring robust support for their distinction. This study explores rare family Diaporthostomataceae, providing first descriptions anamorphic forms. By offering detailed data, research lays foundation future taxonomic systematic Diaporthales.

Language: Английский

Citations

1
Morpho-phylogenetic evidence reveals four novel species of Coniella (Diaporthales, Schizoparmaceae) from southern China DOI Creative Commons

Duhua Li,

Zixu Dong,

Qiyun Liu

et al.

MycoKeys, Journal Year: 2025, Volume and Issue: 116, P. 1 - 23

Published: April 4, 2025

Coniella species are distributed worldwide and have been reported as plant pathogens, endophytes, or saprobes. In our ongoing survey of terrestrial fungi in southern China, we obtained isolates from diseased leaf tissues Fujian, Hainan, Yunnan provinces. Maximum likelihood Bayesian inference based on four loci (ITS, LSU, rpb2, tef1-α) were used to clarify the taxonomic placement species. We confirmed that they represent new species, namely Conielladiaoluoshanensis, C.dongshanlingensis, C.grossedentatae, C.veri both morphology phylogeny support. The compared with other comprehensive descriptions micrographs provided.

Language: Английский

Citations

0
New entomopathogenic species in the Clavicipitaceae family (Hypocreales, Ascomycota) from the subtropical forests of Fujian, China DOI Creative Commons

Longbing Lin,

Yongsheng Lin,

Nemat O. Keyhani

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 3, 2025

Introduction Entomopathogenic fungi play a crucial role in the ecological regulation of insect populations and can be exploited as resource for pest control, sustainable agriculture, natural products discovery. These their infected hosts are sometimes highly coveted part traditional medicine practices. Here, we sought to examine biodiversity entomogenous subtropical forests China. Methods Fungal-infected specimens were collected from various sites Fujian Province, China, purified isolates obtained through laboratory cultivation isolation techniques. Molecular characterization specific target genomic loci was performed on fungal isolates, used phylogenetic analyses using Bayesian inference maximum likelihood methods elucidate taxonomic relationships. Microscopy describe morphological features isolates. Results Through comprehensive two-year survey Province via multilocus molecular analysis targeting nr SSU , LSU tef1-α rpb1, rpb2 specimens, identified three novel species within Clavicipitaceae herein described as: Albacillium fuzhouense sp. nov., Conoideocrella gongyashanensis nov. Neoaraneomyces wuyishanensis well recently recorded, Metarhizium cicadae . Each new also distinguished its closest relatives by unique characteristics. Discussion discoveries enrich our understanding family contribute development control strategies

Language: Английский

Citations

0