Biomaterials, Journal Year: 2024, Volume and Issue: 314, P. 122853 - 122853
Published: Sept. 27, 2024
Language: Английский
Applied Materials Today, Journal Year: 2025, Volume and Issue: 44, P. 102786 - 102786
Published: May 19, 2025
Citations
0
Diseases, Journal Year: 2024, Volume and Issue: 12(10), P. 231 - 231
Published: Oct. 1, 2024
Acute adverse reactions to COVID-19 mRNA vaccines are a major concern, as autopsy reports indicate that deaths most commonly occur on the same day of or one following vaccination. These acute may be due cytokine storms triggered by lipid nanoparticles (LNPs) and anaphylaxis induced polyethene glycol (PEG), both which vital constituents mRNA-LNP vaccines. Kounis syndrome, in triggers coronary syndrome (ACS), also responsible for these cardiovascular events. Furthermore, encompass adjuvants, such LNPs, trigger inflammatory cytokines, including interleukin (IL)-1β IL-6. produce spike proteins facilitate release cytokines. Apart from this, histamine released mast cells during allergic plays critical role IL-6 secretion, intensifies responses. In light events, early reduction IL-1β is imperative managing post-vaccine storms, ACS, myocarditis. Corticosteroids can restrict cytokines mitigate responses, while colchicine, known its IL-1β-reducing capabilities, could prove effective. The anti-IL-6 antibody tocilizumab displays promising treatment syndrome. Aside significance treating anaphylaxis, epinephrine induce artery spasms myocardial ischemia making accurate diagnosis essential. upcoming self-amplifying contain LNPs. Given cause storm post vaccination, it crucial consider corticosteroids measure levels effective management.
Language: Английский
Citations
3
Vaccines, Journal Year: 2025, Volume and Issue: 13(1), P. 65 - 65
Published: Jan. 13, 2025
Background: Although mRNA vaccines encapsulated in lipid nanoparticles (LNPs) have demonstrated a safety profile with minimal serious adverse events clinical trials, there is opportunity to further reduce reactogenicity. The development of naked could improve vaccine tolerability. Naked nucleic acid delivery using the jet injection method may be solution. Methods: In first part study, optimal conditions providing low traumatization and high expression model mRNA-GFP molecule tissues laboratory animals were determined. Then, we used selected protocol immunize BALB/c mice mRNA-RBD encoding SARS-CoV-2 receptor-binding domain (RBD). It was that vaccinated developed level specific antibodies virus-neutralizing activity. also induced strong RBD-specific T-cell response reduced viral load lungs after infection virus. immune immunized spring-loaded injector comparable LNPs. Results: this efficacy an inexpensive, simple, safe evaluated validated. Conclusions: Our findings suggest possible alternative LNPs for delivering against infection.
Language: Английский
Citations
0
The Cancer Journal, Journal Year: 2025, Volume and Issue: 31(2)
Published: March 1, 2025
mRNA vaccines consist of antigen-encoding mRNA, which produces the antigenic protein upon translation. Coupling antigen production with innate immune activation can generate a potent, antigen-specific T-cell response. Clinical reports have demonstrated ability to elicit an anticancer response against various tumor types. Here, we discuss strategies enhance potency vaccines. We provide overview existing knowledge regarding and trafficking mechanisms share optimization boost mRNA-mediated production. In addition, address methods target dendritic cells lymph nodes, key initiators Finally, review for enhancing using adjuvants compatible offer unique advantages that be utilized oncology applications. However, significant work is needed understand their underlying develop technologies improve effectiveness.
Language: Английский
Citations
0
Vaccines, Journal Year: 2024, Volume and Issue: 12(5), P. 538 - 538
Published: May 14, 2024
The development of a safe and effective vaccine against avian influenza A virus (AIV) H5N8 is relevant due to the widespread distribution this in bird population existing potential risk human infection, which can lead significant public health concerns. Here, we developed an experimental pVAX-H5 DNA encoding modified trimer AIV hemagglutinin. Immunization BALB/c mice with using jet injection elicited high titer antibody response (the average ELISA was 1 × 105), generated level neutralizing antibodies T-cell response, as determined by ELISpot analysis. Both liquid lyophilized forms provided 100% protection immunized lethal challenge A/turkey/Stavropol/320-01/2020 (H5N8). results obtained indicate that has good opportunities candidate
Language: Английский
Citations
2
Vaccines, Journal Year: 2024, Volume and Issue: 12(7), P. 757 - 757
Published: July 9, 2024
mRNA vaccines were successfully developed and approved for emergency use to fight coronavirus disease 2019. However, the effect of DNA against SARS-CoV-2 is considerably lower than that vaccines. A pyro-drive jet injector (PJI) efficiently delivers plasmid intradermally into animal models. Here, we compared immunogenic potential in mice using same platform. PJI was used deliver naked pDNA their efficacy inducing antigen expression immune responses assessed. Our results showed delivered skin, a smaller effective dose injection required achieve similar levels expression. The PJI-delivered CpG-free vaccine induced antigen-specific antibody production cell-mediated IFN-γ response vaccine, as well upregulation inflammatory cytokines (IL-6, IFN-γ, IL-1β) skin lymph nodes. intradermal significantly less standard intramuscular mRNA-lipid nanoparticle despite equivalent dosages. Improvements lipid technology have revolutionized vaccines, can be similarly modified higher clinical efficacy.
Language: Английский
Citations
1
Biomaterials, Journal Year: 2024, Volume and Issue: 314, P. 122853 - 122853
Published: Sept. 27, 2024
Language: Английский
Citations
0