Neonatal SARS‐CoV‐2 mRNA Vaccination Efficacy Is Influenced by Maternal Antibodies DOI Creative Commons

Amy Schumer,

Elizabeth A. Bonney,

Ethan Harby

et al.

American Journal of Reproductive Immunology, Journal Year: 2024, Volume and Issue: 92(4)

Published: Oct. 1, 2024

ABSTRACT Problem Vaccination in pregnancy guards against infection. Maternal antibodies, however, can inhibit antibody production neonates. We sought to determine the effects of maternal vaccination on neonatal immune response a SARS‐CoV‐2 mRNA vaccine. Method Study hypothesized that mRNA‐lipid nanoparticles (LNP) allows for de novo even presence vertically transmitted antibodies. Female mice were vaccinated with spike receptor binding domain (RBD) mRNA‐LNPs. Mice then bred, and 21‐day‐old pups inoculated same Spike‐specific IgG ELISAs performed using mouse serum. A protein peptide library perform characterized high affinity domains within protein. Results analyzed one‐way ANOVAs Tukey's multiple comparisons tests. Compared unvaccinated dams, there levels spike‐specific detected dams at 3 weeks life ( p < 0.0001). After vaccination, had higher serum than 12 0.001). Antibody specificity moieties RBD similar when comparing dam her pup Week life, different affinities observed by 15 life. Conclusions Pre‐existing antibodies may partially blunt initial mRNA‐LNPs vaccination. This vaccine strategy, does not prohibit subsequent development broad range specificities be protective.

Language: Английский

Novel mRNA vaccines induce potent immunogenicity and afford protection against tuberculosis DOI Creative Commons
Christopher J. De Voss, Marcellus Korompis, Shuailin Li

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 13, 2025

Introduction Mycobacterium tuberculosis ( Mtb ) is the causative agent of (TB), a disease with severe global burden. The intractability has prevented identification clear correlates protection against TB and hindered development novel vaccines that are urgently required. Lipid nanoparticle (LNP)-formulated mRNA highly promising vaccine platform yet to be thoroughly applied TB. Methods We selected five antigens (PPE15, ESAT6, EspC, EsxI, MetE) evaluated their potential as LNP-formulated vaccines, both when each antigen was delivered individually, all were combined in mix regimen (m-Mix). Results Each construct demonstrated unique cellular humoral immunogenicity, m-Mix, well single conferred significant murine challenge model. Whilst potent immune responses maintained boost BCG, there no additional increase efficacy BCG. Combination m-Mix recombinant, replication-deficient chimpanzee adenovirus (ChAdOx1), heterologous prime-boost delivery (C-m-Mix), appeared result increased upon infection, than either alone. Discussion This work warrants further investigation for TB, whilst indicating C-m-Mix progress stages development.

Language: Английский

Citations

0
Translational research on pandemic virus infection using nonhuman primate models DOI
Hirohito Ishigaki, Yasushi Itoh

Virology, Journal Year: 2025, Volume and Issue: unknown, P. 110511 - 110511

Published: March 1, 2025

Language: Английский

Citations

0
Broad protection and respiratory immunity of dual mRNA vaccination against SARS-CoV-2 variants DOI Creative Commons
Renee L. Hajnik, Jessica A. Plante, Srinivasa Reddy Bonam

et al.

npj Vaccines, Journal Year: 2024, Volume and Issue: 9(1)

Published: Sept. 4, 2024

Language: Английский

Citations

2
The roles of CD4+ T cell help, sex, and dose in the induction of protective CD8+ T cells against a lethal poxvirus by mRNA-LNP vaccines DOI Creative Commons
Samita Kafle, Brian Montoya, Lingjuan Tang

et al.

Molecular Therapy — Nucleic Acids, Journal Year: 2024, Volume and Issue: 35(3), P. 102279 - 102279

Published: July 20, 2024

The role of CD4

Language: Английский

Citations

1
Neonatal SARS‐CoV‐2 mRNA Vaccination Efficacy Is Influenced by Maternal Antibodies DOI Creative Commons

Amy Schumer,

Elizabeth A. Bonney,

Ethan Harby

et al.

American Journal of Reproductive Immunology, Journal Year: 2024, Volume and Issue: 92(4)

Published: Oct. 1, 2024

ABSTRACT Problem Vaccination in pregnancy guards against infection. Maternal antibodies, however, can inhibit antibody production neonates. We sought to determine the effects of maternal vaccination on neonatal immune response a SARS‐CoV‐2 mRNA vaccine. Method Study hypothesized that mRNA‐lipid nanoparticles (LNP) allows for de novo even presence vertically transmitted antibodies. Female mice were vaccinated with spike receptor binding domain (RBD) mRNA‐LNPs. Mice then bred, and 21‐day‐old pups inoculated same Spike‐specific IgG ELISAs performed using mouse serum. A protein peptide library perform characterized high affinity domains within protein. Results analyzed one‐way ANOVAs Tukey's multiple comparisons tests. Compared unvaccinated dams, there levels spike‐specific detected dams at 3 weeks life ( p < 0.0001). After vaccination, had higher serum than 12 0.001). Antibody specificity moieties RBD similar when comparing dam her pup Week life, different affinities observed by 15 life. Conclusions Pre‐existing antibodies may partially blunt initial mRNA‐LNPs vaccination. This vaccine strategy, does not prohibit subsequent development broad range specificities be protective.

Language: Английский

Citations

1