Evaluation of TRPM2 Channel‐Mediated Autophagic Signaling Pathway in Hippocampus and Cortex Tissues of Rat Offspring Following Prenatal Exposure to Elevated Alcohol Levels DOI Creative Commons
Abdülhadi Cihangir Uğuz, Aslı Okan, Züleyha Doğanyiğit

et al.

Environmental Toxicology, Journal Year: 2024, Volume and Issue: 40(2), P. 222 - 244

Published: Oct. 10, 2024

ABSTRACT Fetal alcohol syndrome (FAS) can occur because of high amount intake during pregnancy and is characterized by both physical neurological problems. Children diagnosed with FAS have difficulties in learning, memory, coordination. Hippocampus has a major role memory learning. We aimed to determine whether exposure had any effect on offspring evaluating learning ability as well oxidative stress autophagy the hippocampus cortex tissues litters. Attention was also paid sex differences. To do so, TRPM2, Beclin1, p62, LC3B, IBA1, parvalbumin, GAD65, mGluR5 expression levels were evaluated immunohistochemistry. Lactate dehydrogenase (LDH), malondialdehyde (MDA) levels, total oxidant (TOS) antioxidant (TAS) status determined ELISA. Learning experiments Morris water maze (MWM) test. Our findings demonstrated that higher female rats chronic (CAE) model. IHC results revealed TRPM2 significantly increased males females CAE group. Likewise, TAS lower, TOS animals. Moreover, MWM outcomes supported deficiency litters compared controls indicated outperformed experiments. Therefore, our detrimental effects signaling tissue litters, which could affect

Language: Английский

Parvalbumin interneurons in human ventromedial prefrontal cortex: a comprehensive post-mortem study of myelination and perineuronal nets in neurotypical individuals and depressed suicides with and without a history of child abuse DOI
Stéphanie Théberge, Claudia Belliveau,

Dongyue Xie

et al.

Cerebral Cortex, Journal Year: 2024, Volume and Issue: 34(5)

Published: May 1, 2024

Cortical parvalbumin interneurons (PV+) are major regulators of excitatory/inhibitory information processing, and their maturation is associated with the opening developmental critical periods (CP). Recent studies reveal that cortical PV+ axons myelinated, myelination along perineuronal net (PNN) around cells closures CP. Although susceptible to early-life stress, relationship between PNN coverage remains unexplored. This study compared fine features in well-characterized human post-mortem ventromedial prefrontal cortex samples (n = 31) from depressed suicides or without a history child abuse (CA) matched controls. In healthy controls, 81% all sampled displayed myelinated axon, while subset (66%) these also PNN, proposing both attributes. Intriguingly, 3-fold increase proportion unmyelinated was observed CA victims, greater PV-immunofluorescence intensity PNN. study, which first provide normative data on PNNs neocortex, sheds further light cellular molecular consequences adversity interneurons.

Language: Английский

Citations

1

Active stress resilience DOI

Maja Snippe-Strauss,

Adi Tenenhaus-Zamir,

Amir Benhos

et al.

Current Opinion in Behavioral Sciences, Journal Year: 2024, Volume and Issue: 58, P. 101403 - 101403

Published: May 23, 2024

Language: Английский

Citations

1

Acute stress causes sex-dependent changes to ventral subiculum synapses, circuitry, and anxiety-like behavior DOI Creative Commons
Carley N. Miller, Yuan Li, Kevin T. Beier

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 3, 2024

Abstract Experiencing a single severe stressor is sufficient to drive sexually dimorphic psychiatric disease development. The ventral subiculum (vSUB) emerges as site where stress may induce adaptations due its sex-specific organization and pivotal role in integration. Using 1-hr acute restraint model, we uncover that causes net decrease vSUB activity females potent, long-lasting, driven by adrenergic receptor signaling. By contrast, males exhibit increase transient corticosterone We further identified sex-dependent changes output the bed nucleus of stria terminalis anxiety-like behavior response stress. These findings reveal striking disease-relevant brain regions following with sex-, cell-type, synapse-specificity contribute our understanding shape stress-related risk. Highlights BS cells are uniquely sensitive Stress cell E/I balance aBNST vivo females, but not

Language: Английский

Citations

1

Long term effects of peripubertal stress on the thalamic reticular nucleus of female and male mice DOI Creative Commons
Julia Alcaide,

Yaiza Gramuntell,

Patrycja Klimczak

et al.

Neurobiology of Disease, Journal Year: 2024, Volume and Issue: 200, P. 106642 - 106642

Published: Aug. 22, 2024

Adverse experiences during infancy and adolescence have an important enduring effect on the brain are predisposing factors for mental disorders, particularly major depression. This impact is notable in regions with protracted development, such as prefrontal cortex. The inhibitory neurons of this cortical region altered by peripubertal stress (PPS), female mice. In study we explored whether circuits thalamus impacted PPS male diencephalic structure, cortex, also completes its development postnatal life affected adverse experiences. long-term changes induced were exclusively found adult We that increases depressive-like behavior induces parvalbumin-expressing (PV+) cells thalamic reticular nucleus (TRN). observed reductions volume TRN, together those parameters related to structures/molecules regulate plasticity connectivity PV+ cells: perineuronal nets, matricellular structures surrounding neurons, polysialylated form neural cell adhesion molecule (PSA-NCAM). expression GluN1, but not GluN2C, NMDA receptor subunit was augmented TRN after PPS. An increase fluorescence intensity puncta synaptic output lateral posterior nucleus. These results demonstrate thalamus, vulnerable effects aversive early life, females. vulnerability probably might contribute psychiatric disorders.

Language: Английский

Citations

1

Evaluation of TRPM2 Channel‐Mediated Autophagic Signaling Pathway in Hippocampus and Cortex Tissues of Rat Offspring Following Prenatal Exposure to Elevated Alcohol Levels DOI Creative Commons
Abdülhadi Cihangir Uğuz, Aslı Okan, Züleyha Doğanyiğit

et al.

Environmental Toxicology, Journal Year: 2024, Volume and Issue: 40(2), P. 222 - 244

Published: Oct. 10, 2024

ABSTRACT Fetal alcohol syndrome (FAS) can occur because of high amount intake during pregnancy and is characterized by both physical neurological problems. Children diagnosed with FAS have difficulties in learning, memory, coordination. Hippocampus has a major role memory learning. We aimed to determine whether exposure had any effect on offspring evaluating learning ability as well oxidative stress autophagy the hippocampus cortex tissues litters. Attention was also paid sex differences. To do so, TRPM2, Beclin1, p62, LC3B, IBA1, parvalbumin, GAD65, mGluR5 expression levels were evaluated immunohistochemistry. Lactate dehydrogenase (LDH), malondialdehyde (MDA) levels, total oxidant (TOS) antioxidant (TAS) status determined ELISA. Learning experiments Morris water maze (MWM) test. Our findings demonstrated that higher female rats chronic (CAE) model. IHC results revealed TRPM2 significantly increased males females CAE group. Likewise, TAS lower, TOS animals. Moreover, MWM outcomes supported deficiency litters compared controls indicated outperformed experiments. Therefore, our detrimental effects signaling tissue litters, which could affect

Language: Английский

Citations

1