The Dual Role of Exogenous Hydrogen Sulfide (H2S) in Intestinal Barrier Mitochondrial Function: Insights into Cytoprotection and Cytotoxicity Under Non-Stressed Conditions DOI Creative Commons
Domenica Mallardi, Guglielmina Chimienti, Fatima Maqoud

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(4), P. 384 - 384

Published: March 25, 2025

Hydrogen sulfide (H2S) is a critical gasotransmitter that plays dual role in physiological and pathological processes, particularly the gastrointestinal tract. While levels of H2S exert cytoprotective effects, excessive concentrations can lead to toxicity, oxidative stress, inflammation. The aim this study was investigate dose-dependent effects exogenous on mitochondrial functions biogenesis intestinal epithelial cells under non-stressed conditions. Using Caco-2 monolayer model, we evaluated impact sodium hydrosulfide (NaHS) at ranging from 1 × 10−7 M 5 10−3 metabolism, redox balance, antioxidant defense, inflammatory responses, autophagy/mitophagy, apoptosis. Our results demonstrated biphasic response: low-to-moderate (1 M–1.5 M) enhance through PGC-1α activation, upregulating TFAM COX-4 expression, increasing mtDNA copy number. In contrast, higher (2 10−3–5 impair function, induce promote These are associated with elevated reactive oxygen species (ROS) production, dysregulation enzymes, COX-2-mediated H2S-induced autophagy/mitophagy protective mechanism intermediate but fails mitigate damage toxic levels. This underscores delicate balance between cytotoxic cells, helping develop new therapeutic approaches for disorders.

Language: Английский

The Dual Role of Exogenous Hydrogen Sulfide (H2S) in Intestinal Barrier Mitochondrial Function: Insights into Cytoprotection and Cytotoxicity Under Non-Stressed Conditions DOI Creative Commons
Domenica Mallardi, Guglielmina Chimienti, Fatima Maqoud

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(4), P. 384 - 384

Published: March 25, 2025

Hydrogen sulfide (H2S) is a critical gasotransmitter that plays dual role in physiological and pathological processes, particularly the gastrointestinal tract. While levels of H2S exert cytoprotective effects, excessive concentrations can lead to toxicity, oxidative stress, inflammation. The aim this study was investigate dose-dependent effects exogenous on mitochondrial functions biogenesis intestinal epithelial cells under non-stressed conditions. Using Caco-2 monolayer model, we evaluated impact sodium hydrosulfide (NaHS) at ranging from 1 × 10−7 M 5 10−3 metabolism, redox balance, antioxidant defense, inflammatory responses, autophagy/mitophagy, apoptosis. Our results demonstrated biphasic response: low-to-moderate (1 M–1.5 M) enhance through PGC-1α activation, upregulating TFAM COX-4 expression, increasing mtDNA copy number. In contrast, higher (2 10−3–5 impair function, induce promote These are associated with elevated reactive oxygen species (ROS) production, dysregulation enzymes, COX-2-mediated H2S-induced autophagy/mitophagy protective mechanism intermediate but fails mitigate damage toxic levels. This underscores delicate balance between cytotoxic cells, helping develop new therapeutic approaches for disorders.

Language: Английский

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