Automatic case cluster detection using hospital electronic health record data DOI Creative Commons
Michael DeWitt, Thomas F. Wierzba

Biology Methods and Protocols, Journal Year: 2023, Volume and Issue: 8(1)

Published: Jan. 1, 2023

Case detection through contact tracing is a key intervention during an infectious disease outbreak. However, intensive process where given tracer must locate not only confirmed cases but also identify and interview known contacts. Often these data are manually recorded. During emerging outbreaks, the number of contacts could expand rapidly beyond this, when focused on individual transmission chains, larger patterns may be identified. Understanding if particular can clustered linked to common source help prioritize effects understand underlying risk factors for large spreading events. Electronic health records systems used by vast majority private healthcare across USA, providing potential way automatically detect outbreaks connect already collected data. In this analysis, we propose algorithm case clusters within community outbreak using Bayesian probabilistic linking explore how approach supplement responses; especially human resources limited.

Language: Английский

Wastewater pathogen surveillance based on One Health approach DOI Creative Commons
Kangpeng Xiao, Longxian Zhang

The Lancet Microbe, Journal Year: 2023, Volume and Issue: 4(5), P. e297 - e297

Published: Feb. 24, 2023

Language: Английский

Citations

28
Seasonality of respiratory, enteric, and urinary viruses revealed by wastewater genomic surveillance DOI Creative Commons

Matthew F. Smith,

R Maqsood,

Regan A. Sullins

et al.

mSphere, Journal Year: 2024, Volume and Issue: 9(5)

Published: May 7, 2024

ABSTRACT Wastewater surveillance can reveal population-level infectious disease burden and emergent public health threats be reliably assessed through wastewater surveillance. While molecular methods for monitoring of microorganisms have traditionally relied on PCR-based approaches, next-generation sequencing (NGS) provide deeper insights via genomic analyses multiple diverse pathogens. We conducted a year-long 1,408 composite samples collected from 12 neighborhood-level access points in the greater Tempe area, Arizona, USA, show that variation viruses is driven by seasonal time location. The temporal dynamics were influenced cyclically, with most dissimilarity between 23 weeks apart (i.e., winter vs summer, spring fall). identified urinary enteric including polyomaviruses, astroviruses, noroviruses, showed their genotypes/subtypes shifted across seasons. while data certain respiratory like severe acute syndrome coronavirus 2 (SARS-CoV-2) strongly correlate clinical case rates, laboratory-reported incidences discordant surges high viral load other human 229E. These results demonstrate utility informing decision-making health. IMPORTANCE into spread pathogens communities. Advances methodologies allow more precise detection wastewater. Long-term an important tool preparedness. This system act as observatory gives real-time early warning outbreaks improved response times.

Language: Английский

Citations

9
Wastewater-based epidemiology to assess environmentally influenced disease DOI Creative Commons
Devin A. Bowes, Erin M. Driver, Phil M. Choi

et al.

Journal of Exposure Science & Environmental Epidemiology, Journal Year: 2024, Volume and Issue: 34(3), P. 387 - 388

Published: May 1, 2024

Wastewater-based epidemiology (WBE), also known as wastewater-based surveillance, is a rapidly evolving scientific discipline that leverages community wastewater to assess population health in near real-time [1].This accomplished by measuring captured human excreted biomarkers composited are indicative of various aspects health, behavior, exposure, and activity.Since the early 2000s, WBE has been used beneficial tool for monitoring population-level substance use, most recently gained widespread public exposure during COVID-19 global pandemic, where SARS-CoV-2 reaffirmed early-warning capabilities potential reveal hotspots infection not otherwise detected individualized clinical surveillance [2].These applications demonstrated inclusive, minimally invasive, cost-effective, benefits WBE.The articles included Journal Exposure Science Environmental Epidemiology Special Topic: "Wastewater-based Assess Environmentally Influenced Disease" cover range topics explore interconnectedness between humans environment using analysis, including: (i) establishing new targets assessments from wastewater, particularly infectious diseases, (ii) conducting simultaneous measurements chemical biological indicators exposures associated impact, (iii) evaluating utility wastewater-informed data prompt contextually-relevant interventions vulnerable populations it relates environmental connectivity rural, lowresource, non-sewered settings.

Language: Английский

Citations

4
Evaluation of Three Viral Capsid Integrity qPCR Methods for Wastewater-Based Viral Surveillance DOI Creative Commons
Jessica L. Kevill, Kata Farkas,

Kate Herridge

et al.

Food and Environmental Virology, Journal Year: 2025, Volume and Issue: 17(1)

Published: Jan. 6, 2025

Abstract Capsid Integrity qPCR (CI-qPCR) assays offer a promising alternative to cell culture-based infectivity for assessing pathogenic human virus viability in wastewater. This study compared three CI-qPCR methods: two novel (Crosslinker, TruTiter) and one established (PMAxx dye). These methods were evaluated on heat-inactivated non-heat-inactivated ‘live’ viruses spiked into phosphate-buffered saline ( PBS) wastewater, as well naturally present wastewater samples. The viral panel included Human adenovirus 5 (HAdV), enterovirus A71 (EV), hepatitis-A (HAV), influenza-A H3N2 (IAV), respiratory syncytial A2 (RSV), norovirus GI, GII, SARS-CoV-2. All successfully differentiated between degraded, heat-inactivated, live PBS. While all comparable HAdV PMAxx detected significantly lower gene copies EV IAV. In yielded (HAdV, EV, HAV, IAV, RSV) the Crosslinker TruTiter methods. For (un-spiked), no significant difference was observed Intact, potentially infectious using both untreated treated A comparative analysis of data TEM images revealed that flocculation IAV may interfere with capsid integrity intercalating dyes. summary, our findings not only advance development more effective but also highlight potential techniques enhance early warning systems emerging pathogens, thereby strengthening public health preparedness response strategies.

Language: Английский

Citations

0
Wastewater-borne markers of neurodegenerative disease: β-methylamino-L-alanine and aminomethylphosphonic acid DOI
Melanie Engstrom Newell,

Ayesha Babbrah,

Anumitha Aravindan

et al.

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 970, P. 179032 - 179032

Published: March 1, 2025

Language: Английский

Citations

0
Integrated multiomic wastewater-based epidemiology can elucidate population-level dietary behaviour and inform public health nutrition assessments DOI Open Access
Devin A. Bowes, Erin M. Driver,

Sonja Savic

et al.

Nature Food, Journal Year: 2023, Volume and Issue: 4(3), P. 257 - 266

Published: March 13, 2023

Language: Английский

Citations

9
Encrypted data-sharing for preserving privacy in wastewater-based epidemiology DOI
Erin M. Driver,

Manazir Ahsan,

Lucas Piske

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 940, P. 173315 - 173315

Published: May 17, 2024

Language: Английский

Citations

3
Longitudinal wastewater surveillance of four key pathogens during an unprecedented large-scale COVID-19 outbreak in China facilitated a novel strategy for addressing public health priorities–A proof of concept study DOI
Songzhe Fu, Yixiang Zhang, Rui Wang

et al.

Water Research, Journal Year: 2023, Volume and Issue: 247, P. 120751 - 120751

Published: Oct. 19, 2023

Language: Английский

Citations

8
Analyzing community wastewater in sub-sewersheds for the small-scale detection of SARS-CoV-2 variants in a German metropolitan area DOI Creative Commons
Dennis Schmiege, Ivana Kraiselburd, Timo Haselhoff

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 898, P. 165458 - 165458

Published: July 14, 2023

Wastewater surveillance of SARS-CoV-2 proved useful, including for identifying the local appearance newly identified virus variants. Previous studies focused on wastewater treatment plants (WWTP) with sewersheds several hundred thousand people or at single building level, representing only a small number people. Both approaches may prove inadequate small-scale intra-urban inferences early detection emerging novel Our study aims (i) to analyze nucleotide variants (SNVs) in sub-sewersheds and WWTP using whole genome sequencing order (ii) investigate potential known concern (VOC) within metropolitan system. We selected three sub-sewershed sampling sites, based estimated population- built environment-related indicators, inlet receiving Ruhr region, Germany. Untreated was sampled weekly between October December 2021, total 22 samples collected. RNA analyzed by RT-qPCR sequencing. For all samples, sequences were obtained, while 13 positive RT-qPCR. multiple specific SNVs WWTP. Identified reflected dominance VOC Delta time sampling. Interestingly, we could identify an Omicron-specific SNV one sub-sewershed. A concurrent same detected Omicron week later. observations suggest that approach particularly useful description spatially confined existing circulating populations. Future applying strategies taking into account features system will be temporal spatial variance more detail.

Language: Английский

Citations

7
Enhanced detection of mpox virus in wastewater using a pre-amplification approach: A pilot study informing population-level monitoring of low-titer pathogens DOI Creative Commons
Devin A. Bowes, Katherine B. Henke, Erin M. Driver

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 903, P. 166230 - 166230

Published: Aug. 11, 2023

Language: Английский

Citations

7