Resolving the Orientations of and Angular Separation Between a Pair of Dipole Emitters
Physical Review Letters,
Journal Year:
2025,
Volume and Issue:
134(9)
Published: March 7, 2025
We
prove
that
it
is
impossible
to
distinguish
two
spatially
coinciding
fluorescent
molecules
from
a
single
rotating
molecule
using
polarization-sensitive
imaging,
even
if
one
modulates
the
polarization
of
illumination
or
detection
dipole-spread
function
(DSF).
If
target
known
be
dipole
pair,
existing
imaging
methods
perform
poorly
for
measuring
their
angular
separation.
propose
simultaneously
modulating
excitation
and
DSF,
which
demonstrates
robust
discrimination
between
pairs
versus
molecules.
Our
method
improves
precision
centroid
orientation
by
50%
separation
two-
four-fold
over
techniques.
Language: Английский
Monitoring Dynamic Conformations of a Single Fluorescent Molecule Inside a Protein Cavity
Small Methods,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 2, 2025
Abstract
Fluorescence
nanoscopy
and
single‐molecule
methods
are
entering
the
realm
of
structural
biology,
breaking
new
ground
for
dynamic
measurements
at
room
temperature
liquid
environments.
Here,
localization
microscopy,
polarization‐dependent
excitation,
protein
engineering
combined
to
determine
orientation
a
fluorophore
forming
hydrogen
bonds
inside
cavity.
The
observed
conformations
in
good
agreement
with
molecular
dynamics
simulations,
enabling
new,
more
realistic
interplay
between
experiments
simulations
identify
stable
key
interactions
involved.
Furthermore,
jumps
can
be
monitored
precision
3°
time
resolution
few
seconds,
confirming
potential
this
methodology
retrieving
information
nanoscopic
biological
systems
under
physiologically
compatible
conditions.
Language: Английский
Painting rich six-dimensional pictures using polarized fluorescence microscopy
Proceedings of the National Academy of Sciences,
Journal Year:
2025,
Volume and Issue:
122(14)
Published: March 31, 2025
Emotions
coordinate
our
behavior
and
physiological
states
during
survival-salient
events
pleasurable
interactions.
Even
though
we
are
often
consciously
aware
of
current
emotional
state,
such
as
anger
or
happiness,
the
mechanisms
giving
...Emotions
felt
in
body,
somatosensory
feedback
has
been
proposed
to
trigger
conscious
experiences.
Here
reveal
maps
bodily
sensations
associated
with
different
emotions
using
a
unique
topographical
self-report
method.
In
...
Language: Английский
A Study on Exploring the Composition, Structure and Innovative Analytical Techniques Developed in Biomembrane Research
Biosciences Biotechnology Research Asia,
Journal Year:
2025,
Volume and Issue:
22(1), P. 63 - 71
Published: March 25, 2025
ABSTRACT:
Microbial
communities
called
biofilms
are
complex
structures
that
adhere
to
surfaces
and
encased
in
a
matrix
of
extracellular
polymeric
substances
(EPS).
These
formations
found
throughout
nature
play
crucial
role
the
survival
microorganisms
various
environments.
The
development
occurs
several
stages:
first,
there
is
initial
surface
contact;
next
permanent
attachment;
then
small
colonies
formed;
this
followed
by
full
development;
finally,
dispersal.
Various
factors
influence
biofilm
formation,
such
as
substratum
effects,
hydrodynamics,
environmental
conditions.
Biofilms
pose
significant
challenges
healthcare,
food
processing,
other
industries
due
their
increased
resistance
antimicrobial
agents
potential
for
contamination.
Recent
advances
imaging
techniques
have
revolutionized
study
biofilms,
providing
insights
into
structure,
composition,
physiology.
Light
microscopy,
transmission
electron
microscopy
(TEM),
scanning
(SEM),
confocal
laser
(CLSM),
atomic
force
(AFM)
among
most
commonly
used
characterization.
Other
advanced
techniques,
AFM-based
infrared
spectroscopy
(AFM-IR),
fluorescent
situ
hybridization
(FISH),
Raman
spectroscopy,
ATP
bioluminescence,
mass
spectrometry,
quantitative
real-time
PCR
analysis,
Bio
Finder,
offer
complementary
approaches
investigating
chemical
gene
expression.
Choosing
right
method
depends
on
specific
research
goals
required
spatial
temporal
accuracy.
Combining
different
can
provide
comprehensive
understanding
behavior
help
develop
effective
strategies
managing
eliminating
biofilms.
Language: Английский