From capture to detection: A critical review of passive sampling techniques for pathogen surveillance in water and wastewater

Water Research, Journal Year: 2024, Volume and Issue: 261, P. 122024 - 122024

Published: June 30, 2024

Water quality, critical for human survival and well-being, necessitates rigorous control to mitigate contamination risks, particularly from pathogens amid expanding urbanization. Consequently, the necessity maintain microbiological safety of water supplies demands effective surveillance strategies, reliant on collection representative samples precise measurement contaminants. This review critically examines advancements passive sampling techniques monitoring in various systems, including wastewater, freshwater, seawater. We explore evolution conventional materials innovative adsorbents pathogen capture shift culture-based molecular detection methods, underscoring adaptation this field global health challenges. The comparison highlights sampling's efficacy over like grab its potential overcome existing challenges through use such as granular activated carbon, thermoplastics, polymer membranes. By evaluating literature, work identifies standardization gaps proposes future research directions augment efficiency, specificity, utility environmental public surveillance.

Language: Английский

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Campus Source to Sink Wastewater Surveillance of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2).

Current Research in Microbial Sciences, Journal Year: 2024, Volume and Issue: 6, P. 100240 - 100240

Published: Jan. 1, 2024

Wastewater-based surveillance (WBS) offers an aggregate, and cost-effective approach for tracking infectious disease outbreak prevalence within communities, that provides data on community health complementary to individual clinical testing. This study reports a 16-month WBS initiative university campus in England, UK, assessing the presence of SARS-CoV-2 sewers from large buildings, downstream sewer locations, raw wastewater, partially treated effluents. Key findings include detection Alpha (B.1.1.7) variant with 70% confirmed cases correlating positive wastewater samples. Notably, ammonium nitrogen (NH4-N) levels showed correlation (ρ = 0.543, p <0.01) virus at building scale, relationship not observed or treatment works (WWTW) due dilution. The WWTW was compliant standards, but secondary processes were efficient removal as consistently detected discharges. Tools developed through can also be used enhance traditional environmental monitoring aquatic systems. detailed source-to-sink evaluation, emphasizing critical need widespread application improvement WBS. It showcases utility reinforces ongoing challenges posed by viruses receiving water quality.

Language: Английский

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Use of wastewater from passenger ships to assess the movement of COVID-19 and other pathogenic viruses across maritime international boundaries

Frontiers in Public Health, Journal Year: 2024, Volume and Issue: 12

Published: July 15, 2024

The worldwide spread of SARS-CoV-2 and the resulting COVID-19 pandemic has been driven by international travel. This led to desire develop surveillance approaches which can estimate rate import pathogenic organisms across borders. aim this study was investigate use wastewater-based for viral pathogens on commercial short-haul (3.5 h transit time) roll-on/roll-off passenger/freight ferries operating between UK Republic Ireland.

Language: Английский

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Maximizing viral nucleic acid yield from passive samplers: Evaluating elution and extraction protocols

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 957, P. 177834 - 177834

Published: Nov. 30, 2024

The COVID-19 pandemic has underscored the need for effective viral tracking in aqueous environments, particularly non-enteric viruses. Despite advances wastewater monitoring, surveillance of viruses freshwater remains limited due to traditional sampling challenges. This study refines GAC-based passive protocols by determining optimal extraction and elution methods enhancing recovery nucleic acids freshwater. Three commercially available total acid (TNA) kits four buffers were assessed their ability recover SARS-CoV-2 bacteriophage MS2 from samplers. Promega Wizard® Enviro Total Nucleic Acid Kit, paired with a Tween®20-based buffer, provided highest virus efficiency sampling. Field-scale applications demonstrated effectiveness samplers capturing SARS-CoV-2, INFA, RSV, MeV using optimized protocols. combination Tween®20 based buffer kit led increased detection frequencies grab samples, which remained lower than underscores importance selecting appropriate TNA maximize samples. By optimizing these protocols, we enhance sensitivity reliability ecosystems.

Language: Английский

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The anthropic fingerprinting of chemicals in wastewater: Wastewater based-epidemiology

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 691 - 718

Published: Nov. 1, 2024

Language: Английский

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