Biotechnological Approaches on Two High CBD and CBG Cannabis sativa L. (Cannabaceae) Varieties: In Vitro Regeneration and Phytochemical Consistency Evaluation of Micropropagated Plants Using Quantitative 1H-NMR DOI Creative Commons
Kostas Ioannidis, Evangelos Dadiotis,

Vangelis Mitsis

et al.

Molecules, Journal Year: 2020, Volume and Issue: 25(24), P. 5928 - 5928

Published: Dec. 15, 2020

High cannabidiol (CBD) and cannabigerol (CBG) varieties of Cannabis sativa L., a species with medicinal properties, were regenerated in vitro. Explants nodal segments including healthy axillary bud, after sterilization, placed Murashige-Skoog (MS) culture medium. The shoots formed 30 days subcultured full- or half-strength MS medium supplemented several concentrations 6-benzyl-amino-purine (BA) thidiazuron (TDZ). highest average number length was achieved when both full media 4.0 μM BA. presence TDZ showed also comparable results. BA at 4.0, 8.0 2.0, respectively, displayed the maximum shooting frequency. new transferred on same either self-rooted being enhanced different indole-3-butyric acid (IBA) α-naphthalene acetic (NAA). Presence 2.0 IBA NAA resulted to optimum rooting rates. roots per shoot observed NAA. Approximately 92% plantlets successfully established acclimatized field. consistency chemical profile vitro propagated clones assessed using quantitative 1H-NMR high throughput screening. In each variety, analysis micropropagated plant comparison mother no statistically significant differences (p ≤ 0.05) CBD+ cannabidiolic (CBDA) CBG+ cannabigerolic (CBGA) content thus indicating stability their profile.

Language: Английский

Potential of NIRS for determining multiple cannabinoid concentrations in Cannabis sativa L. DOI Creative Commons
Tina Sullivan, Matt A. Yost, J. Earl Creech

et al.

Agrosystems Geosciences & Environment, Journal Year: 2025, Volume and Issue: 8(2)

Published: March 26, 2025

Abstract Following the 2018 Farm Bill, many US states allowed for hundreds of industrial hemp licenses, and resulting production flooded markets. Floral ( Cannabis sativa L.) offered prospects in essential oils medicinal, flavoring, fragrance additives. One major limitation growers is expense Δ9‐tetrahydrocannabinol (Δ9THC) monitoring to meet legal compliance while maximizing cannabidiol (CBD) or cannabigerol (CBG) concentration financial return. Cannabinoid analysis not widely available, shipping samples legally challenging with state‐by‐state legislation. The study objectives were evaluate whether near‐infrared spectroscopy (NIRS) could be used rapidly inexpensively quantify commonly produced cannabinoid concentrations compared industry standard high‐performance liquid chromatography (HPLC). A total 448 tissue from 3 years outdoor cultivar, irrigation, fertility trials evaluated. Samples dried ground, Δ8‐tetrahydrocannabinol (Δ8THC), Δ9THCD, tetrahydrocannabinolic acid (THCA), CBD, CBG, cannabichromene (CBC), cannabinol (CBN), cannabidivarin (CBDV), (CBDA), (CBGA) determined by HPLC a diode array detector as reference measurements, prior NIRS. Calibration equations developed assess NIRS can estimate concentrations. three cannabinoids most importance floral (THCA, CBDA, CBD) had widest range best correlation R 2 = 0.71–0.87) between HPLC, other limited ranges poorer correlation. THC CBD results are highly encouraging indicate that robust tool rapid inexpensive quantification.

Language: Английский

Citations

0
Evaluation of the potential use of cannabidiol in the treatment of cocaine use disorder: A systematic review DOI Creative Commons
Larissa Alencar Rodrigues, Mariana Emanuele Silva Caroba,

Fernando Kengy Taba

et al.

Pharmacology Biochemistry and Behavior, Journal Year: 2020, Volume and Issue: 196, P. 172982 - 172982

Published: July 7, 2020

Cannabinoids may have an important therapeutic potential for the treatment of dependence on crack cocaine. Cannabidiol (CBD), in particular, has anxiolytic, antipsychotic and anticonvulsant properties plays a role regulating motivation circuitry controlling sleep disorders. Several studies were performed evaluating CBD experimental models This systematic review aims evaluate use cocaine disorder.Five databases (Scielo; Medline/PubMed; PsycINFO; Cochrane Library; Virtual Health Library-VHL) searched up to January 2020. Full-text reports published English included if they that administered human and/or adult animals or with history crack/cocaine administration. The risk bias each study selected was appraised by two independent reviewers following Systematic Review Centre Laboratory Animal Experimentation (SYRCLE) protocol.Fifty-one analyzed, 14 selected. No conducted humans found; only one clinical trial ongoing. results grouped into categories: self-administration, brain-stimulation reward, conditioned place preference, neuronal proliferation, anxiety, hepatic protection, effect locomotor sensitization response Only four had low bias. promotes reduction self-administration. Also, it interferes induce brain reward stimulation dopamine release. alteration contextual memory associated neuroadaptations, hepatotoxicity seizures induced cocaine.The evidence indicates is promising adjunct therapy due its on: effects, consumption, behavioral responses, protection safety. Moreover, trials are strongly required determine whether findings animal occur diagnosed disorder.

Language: Английский

Citations

26
Two-weeks treatment with cannabidiol improves biophysical and behavioral deficits associated with experimental type-1 diabetes DOI

Yane Costa Chaves,

Karina Genaro, Cristina Aparecida Jark Stern

et al.

Neuroscience Letters, Journal Year: 2020, Volume and Issue: 729, P. 135020 - 135020

Published: April 30, 2020

Language: Английский

Citations

25
Subacute cannabidiol alters genome‐wide DNA methylation in adult mouse hippocampus DOI

Nicole M. Wanner,

Mathia Colwell,

Chelsea Drown

et al.

Environmental and Molecular Mutagenesis, Journal Year: 2020, Volume and Issue: 61(9), P. 890 - 900

Published: June 24, 2020

Abstract Use of cannabidiol (CBD), the most abundant non‐psychoactive compound found in cannabis ( Cannabis sativa ), has recently increased as a result widespread availability CBD‐containing products. CBD is FDA‐approved for treatment epilepsy and exhibits anxiolytic, antipsychotic, prosocial, other behavioral effects animal studies clinical trials, however, underlying mechanisms governing these phenotypes are still being elucidated. The epigenome, particularly DNA methylation, responsive to environmental input can govern persistent patterns gene regulation affecting phenotype across life course. In order understand epigenomic activity exposure adult brain, 12‐week‐old male wild‐type a/a Agouti viable yellow (A vy ) mice were exposed either 20 mg/kg or vehicle daily by oral administration 14 days. Hippocampal tissue was collected reduced‐representation bisulfite sequencing (RRBS) performed. Analyses revealed 3,323 differentially methylated loci (DMLs) CBD‐exposed animals with small skew toward global hypomethylation. Genes cell adhesion migration, dendritic spine development, excitatory postsynaptic potential be enriched ontology term analysis DML‐containing genes, disease enrichment an overrepresentation DMLs sets associated autism spectrum disorder, schizophrenia, phenotypes. These results suggest that epigenome may key substrate CBD's provides wealth regulatory information further study.

Language: Английский

Citations

25
Biotechnological Approaches on Two High CBD and CBG Cannabis sativa L. (Cannabaceae) Varieties: In Vitro Regeneration and Phytochemical Consistency Evaluation of Micropropagated Plants Using Quantitative 1H-NMR DOI Creative Commons
Kostas Ioannidis, Evangelos Dadiotis,

Vangelis Mitsis

et al.

Molecules, Journal Year: 2020, Volume and Issue: 25(24), P. 5928 - 5928

Published: Dec. 15, 2020

High cannabidiol (CBD) and cannabigerol (CBG) varieties of Cannabis sativa L., a species with medicinal properties, were regenerated in vitro. Explants nodal segments including healthy axillary bud, after sterilization, placed Murashige-Skoog (MS) culture medium. The shoots formed 30 days subcultured full- or half-strength MS medium supplemented several concentrations 6-benzyl-amino-purine (BA) thidiazuron (TDZ). highest average number length was achieved when both full media 4.0 μM BA. presence TDZ showed also comparable results. BA at 4.0, 8.0 2.0, respectively, displayed the maximum shooting frequency. new transferred on same either self-rooted being enhanced different indole-3-butyric acid (IBA) α-naphthalene acetic (NAA). Presence 2.0 IBA NAA resulted to optimum rooting rates. roots per shoot observed NAA. Approximately 92% plantlets successfully established acclimatized field. consistency chemical profile vitro propagated clones assessed using quantitative 1H-NMR high throughput screening. In each variety, analysis micropropagated plant comparison mother no statistically significant differences (p ≤ 0.05) CBD+ cannabidiolic (CBDA) CBG+ cannabigerolic (CBGA) content thus indicating stability their profile.

Language: Английский

Citations

24