Angewandte Chemie International Edition,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Oct. 10, 2024
Photodynamic
therapy
(PDT)
is
a
clinically
approved
therapeutic
modality
that
has
shown
great
potential
for
cancer
treatment.
However,
there
exist
two
major
problems
hindering
PDT
applications:
the
nonspecific
phototoxicity
requiring
patients
to
stay
in
dark
post-PDT,
and
limited
photodynamic
efficiency.
Herein,
we
report
photo-triggered
porphyrin
polyelectrolyte
nanoassembling
(photo-triggered
PPN)
strategy,
which
photosensitizer
photoswitchable
energy
accepter
are
assembled
into
micelles
by
combined
force
of
charge
interaction
metal-ligand
coordination.
The
polyelectrolyte-based
PPN
exhibits
good
biocompatibility,
bestows
unique
"confining
isolated"
inner
microenvironment
fully
overcoming
π-π
stacking
porphyrins
with
significant
efficiency
(123-fold
enhancement).
Due
high
Förster
resonance
transfer
(FRET)
(91.5
%)
between
photoswitch
closed-form,
could
use
light
as
specific
trigger
modulate
closed-
open-form,
manipulate
ACS Nano,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 7, 2025
Only
a
minority
of
patients
benefit
from
current
T-cell-focused
adaptive
immunotherapies,
underscoring
the
need
to
engage
innate
immune
cells,
particularly
macrophages,
for
multilayered
tumor
control.
However,
high-efficacy
therapeutics
capable
orchestrating
multiple
cells
remain
scarce.
Herein,
dual
stimuli-responsive
nanoimmunomodulator
(6EPP@si)
that
caters
specifically
microenvironment
(TME)
is
presented
antitumor
synergy
macrophages
and
T
cells.
Using
functional
polymer-based
carrier,
we
co-deliver
endoplasmic
reticulum
(ER)-localized
photosensitizer
6E
small
interfering
RNA
targeting
CD47
(siCD47)
into
breast
tumors.
Within
acidic
high-glutathione
TME,
6EPP@si
undergoes
self-lysosome
escape
nanocleavage
precise,
on-demand
drug
release.
Consequently,
siCD47
released
cytoplasm
enables
potent
silencing,
while
ER-targeted
induces
immunogenic
cell
death
through
reactive
oxygen
species-based
ER
stress,
triggering
release
damage-associated
molecular
patterns,
including
calreticulin
surface
translocation.
enhances
macrophage
phagocytosis
by
modulating
both
antiphagocytic
prophagocytic
signals
also
promotes
antigen
presentation
activate
In
orthotopic
spontaneous
lung
metastatic
models,
this
combined
approach
demonstrates
robust
effects
effective
antimetastatic
immunity,
offering
meaningful
strategy
simultaneously
enhancing
cancer
immunotherapy.
Small,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 21, 2025
Abstract
Allergic
rhinitis
(AR)
is
the
most
prevalent
global
health
issue,
affecting
approximately
3
billion
people,
with
its
incidence
increasing
annually.
The
current
first‐line
pharmacotherapy
for
symptom
relief
has
limited
efficacy
and
often
results
in
notable
side
effects.
Here,
aza‐BODIPY‐based
nanoparticles
(RH@NPs)
are
developed
that
exhibit
mild
photothermal
therapy
(PTT)
type
I
photodynamic
(PDT)
capabilities.
Enhanced
intramolecular
charge
transfer
induces
NIR‐II
absorption
of
photosensitizer
(RH),
facilitating
deeper
tissue
penetration
augmented
AR
therapy.
Additionally,
use
an
asymmetric
donor–acceptor–acceptor′
configuration
promotes
self‐assembly
RH,
enhancing
intersystem
crossing
ability
enabling
efficient
photophysical
activity.
synergistic
effects
PTT
(enhancing
HSF1
DNA‐binding
activity
to
inhibit
epithelial‐mesenchymal
transition
by
epigenetically
regulating
expression
transition‐associated
genes)
PDT
(activating
NRF2
transcriptional
stimulate
antioxidant
defense
system)
enable
RH@NPs
provide
a
superior
therapeutic
effect
mouse
model
AR.
This
achieved
mechanically
reducing
allergic
response
rather
than
merely
alleviating
symptoms.
Notably,
photosensitizer‐based
physical
demonstrates
enhanced
safety.
study
first
successfully
investigate
application
phototherapy
elucidate
mechanism
action,
offering
novel,
straightforward,
treatment
strategy
ACS Sensors,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 10, 2025
High-fidelity
tracking
of
glycogen
dynamics
in
living
biosystems
is
critical
for
exploring
the
biological
role
metabolism
diseases.
However,
situ
information
on
state
mainly
relies
a
glucose
uptake
fluorescence
probe
2-NBDG,
which
has
proven
to
be
extremely
limited
owing
"always-on"
fluorescence,
short
emission
wavelength,
and
low
signal-to-noise
(S/N)
ratio.
Herein,
we
first
time
demonstrate
metabolic-activated
off-on
through
covalently
attaching
molecular
rotor
with
hydrophilic
at
C-2
position
guarantee
good
miscibility
complete
fluorescence-off
before
metabolic
incorporation
into
particles.
The
Glycogen-Red
achieves
negligible
background
(1/30
times
than
2-NBDG)
an
ultrahigh
S/N
ratio
(61-fold
lighting-up
near-infrared
(NIR)
biosynthesis.
Notably,
our
unique
characteristic
bypassing
washing
steps,
offering
powerful
toolbox
real-time
biosynthesis
super-resolution
mapping
structures
cells.
Advanced Functional Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 6, 2025
Abstract
In
photodynamic
therapy,
traditional
photosensitizers
exhibit
limited
tumor
retention
and
targeting
efficiency,
posing
significant
challenges
to
achieving
precision
efficacy.
this
study,
a
photosensitizer
is
developed
based
on
hemicyanine
dye,
known
as
Icy‐P,
which
features
cationic
structure
that
naturally
targets
mitochondria.
Icy‐P
contains
CYP2J2
recognition
receptors
at
its
active
sites,
enabling
precise
of
cells.
Positioning
ethyl
carbamate
adjacent
the
group
initiates
cascade
reaction
upon
recognition,
resulting
in
formation
quinone
intermediate,
undergoes
1,4‐addition
reaction.
This
facilitates
binding
an
enzyme,
leading
prolonged
time.
The
vivo
residence
time
within
cells
ranges
between
48
h
7
d.
Furthermore,
generates
reactive
oxygen
species
light
exposure,
mitochondrial
damage,
caspase‐1
activation,
gasdermin
D
cleavage.
These
events
ultimately
induce
pyroptosis,
thereby
enhancing
efficacy
cancer
therapy.
Journal of Medicinal Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 13, 2025
Phototherapy,
renowned
for
its
noninvasiveness,
is
widely
employed
in
tumor
treatment.
However,
the
microenvironment
usually
hypoxic,
with
insufficient
reactive
oxygen
species
(ROS)
production,
severely
limiting
application.
Herein,
we
introduce
a
regio-isomerization
optimization
strategy
and
have
synthesized
four
regio-isomeric
photosensitizers
featuring
donor–acceptor
(D–A)
configuration
by
tactically
varying
linkage
sites
between
D
A.
Among
them,
TAF-3
excellent
photostability
has
an
ultrahigh
type
I
ROS
production
efficiency
(4.79
times
that
of
methylene
blue)
photothermal
conversion
41.7%.
improves
conjugation
degree;
produces
appropriate
intramolecular
charge
transfer
effect,
which
enhances
optical
properties
phototherapeutic
efficiency;
promotes
stronger
immune
cell
death
reducing
postoperative
melanoma
recurrence
60%.
Overall,
attributes
D–A
can
be
tailored
through
precision
modulation
regio-isomerization,
offering
promising
avenue
advancement
clinical
suitable
phototherapy.
Journal of Medicinal Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 1, 2025
NIR-II-triggered
phototherapy
presents
a
noninvasive,
resistance-free
alternative
therapeutic
approach
with
deeper
tissue
penetration
and
improved
imaging
of
deep
tumors.
However,
many
NIR-II
phototherapeutic
agents
suffer
from
low
fluorescence
quantum
yields,
poor
photothermal
conversion
efficiency
(PCE),
reduced
efficacy
due
to
the
upregulation
heat
shock
protein
HSP70.
This
study
develops
small-molecule
agent
(IRF)
high
yield
(17.4%),
excellent
PCE
(96.8%)
for
therapy
(PTT),
photodynamic
(PDT)
activity.
To
decrease
thermal
resistance
during
phototherapy,
IRF
evodiamine
(EVO)
were
loaded
onto
hyaluronic
acid
(HA)-modified
nanoparticles,
creating
multifunctional
nanoplatform
termed
EVO/IRF@HA
NPs.
NPs
can
actively
target
tumors
via
HA
moiety.
Upon
980
nm
laser
irradiation,
increases
temperature
content
reactive
oxygen
species
synergistic
PTT/PDT.
Importantly,
EVO
effectively
inhibits
overexpression
HSP70,
enabling
combined
PTT/PDT/chemotherapy
effective
colorectal
cancer
(CRC)
treatment.
The
overproduction
of
peroxynitrite
(ONOO-)
in
mitochondria
has
been
associated
with
various
pathophysiological
conditions
and
disorders.
However,
the
use
fluorescent
probes
to
visualize
mitochondrial
ONOO-
biological
systems
is
limited
due
their
low
emission
wavelengths
small
Stokes
shifts,
which
present
significant
challenges.
In
this
study,
we
designed
synthesized
julolidine-based
near-infrared
(NIR)
probes,
named
JQMe
JCN,
specifically
monitor
ONOO-.
Comparative
photophysical
studies
revealed
that
exhibits
superior
properties
for
sensing
compared
JCN.
Initially,
emitted
fluorescence
at
706
nm
via
an
intramolecular
charge
transfer
(ICT)
mechanism.
Upon
addition
ONOO-,
NIR
was
suppressed,
resulting
a
rapid
on-off
response
within
5
minutes.
exhibited
high
specific
selectivity
towards
over
other
competing
interferents,
accompanied
by
colorimetric
change
from
deep
blue
colorless.
Additionally,
shift
106
detection
limit
6.5
nM.
proposed
mechanism
validated
through
ESI
mass
spectrometry
DFT
studies.
Furthermore,
successfully
employed
both
endogenous
exogenous
living
cells
using
inducer
inhibitor
tests.
Remarkably,
time-dependent
colocalization
experiments
effectively
targets
reacts
