Nanomaterials-Integrated CRISPR/Cas Systems: Expanding the Toolbox for Optical Detection

ACS Sensors, Journal Year: 2025, Volume and Issue: unknown

Published: April 9, 2025

Nanomaterials-integrated CRISPR/Cas systems have rapidly emerged as powerful next-generation platforms for optical biosensing. These integrated harness the precision of CRISPR/Cas-mediated nucleic acid detection while leveraging unique properties nanomaterials to achieve enhanced sensitivity and expanded analytical capabilities, thereby broadening their diagnostic potential. By incorporating a diverse range nanomaterials, these effectively expand toolbox detection, offering adaptable solutions tailored various challenges. This review provides comprehensive overview successfully into CRISPR/Cas-based sensing systems. It examines multiple modalities, including fluorescence, electrochemiluminescence, colorimetry, surface-enhanced Raman spectroscopy, highlighting how facilitate signal amplification, enable multiplexing, support development point-of-care applications. Additionally, practical applications in critical fields such healthcare diagnostics environmental monitoring are showcased. While offer considerable advantages, several real-world challenges complexity assay workflows, impact cost, regulatory hurdles must be addressed before widespread implementation can achieved. identifying obstacles proposing strategic solutions, we aim pave way continued advancement adoption nanomaterial-integrated biosensing technologies.

Language: Английский

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CRISPR/Cas‐Based MicroRNA Biosensors

Chemistry - A European Journal, Journal Year: 2022, Volume and Issue: 29(16)

Published: Dec. 7, 2022

Abstract As important post‐transcriptional regulators, microRNAs (miRNAs) play irreplaceable roles in diverse cellular functions. Dysregulated miRNA expression is implicated various diseases including cancers, and thus miRNAs have become the valuable biomarkers for disease monitoring. Recently, clustered regularly interspaced short palindromic repeats/CRISPR‐associated (CRISPR/Cas) system has shown great promise development of next‐generation biosensors because its precise localization capability, good fidelity, high cleavage activity. Herein, we review recent advance CRISPR/Cas‐based detection. We summarize principles, features, performance these biosensors, further highlight remaining challenges future directions.

Language: Английский

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High-sensitive and rapid electrochemical detection of miRNA-31 in saliva using Cas12a-based 3D nano-harvester with improved trans-cleavage efficiency

Talanta, Journal Year: 2023, Volume and Issue: 266, P. 125066 - 125066

Published: Aug. 10, 2023

Language: Английский

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A sensitive and rapid electrochemical biosensor for sEV-miRNA detection based on domino-type localized catalytic hairpin assembly

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: Sept. 9, 2023

Abstract Small extracellular–vesicule-associated microRNA (sEV-miRNA) is an important biomarker for cancer diagnosis. However, rapid and sensitive detection of low-abundance sEV-miRNA in clinical samples challenging. Herein, a simple electrochemical biosensor that uses DNA nanowire to localize catalytic hairpin assembly (CHA), also called domino-type localized (DT-LCHA), has been proposed sEV-miRNA1246 detection. The DT-LCHA offers triple amplification, (i). CHA system was nanowire, which shorten the distance between substrate, inducing high collision efficiency H1 H2 domino effect. Then, larger numbers CHAs were triggered, capture probe bind by exposed c sites. (ii) can load large number electroactive substance RuHex as amplified signal tags. (iii) multiple carried only one trapped probe, greatly improve sensitivity, especially when target concentration extremely low. Owing amplification this strategy, at low 24.55 aM be detected 20 min with good specificity. accuracy measurements confirmed using reverse transcription quantitative polymerase chain reaction. Furthermore, platform showed performance discriminating healthy donors from patients early gastric (area under curve [AUC]: 0.96) equally able discriminate benign tumors cancers (AUC: 0.77). Thus, substantial potential biosensing Graphical

Language: Английский

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CRISPR/Cas13a-assisted amplification-free miRNA biosensor via dark-field imaging and magnetic gold nanoparticles

Sensors & Diagnostics, Journal Year: 2024, Volume and Issue: 3(8), P. 1310 - 1318

Published: Jan. 1, 2024

MicroRNAs (miRNAs) are short (about 18-24 nucleotides) non-coding RNAs and have emerged as potential biomarkers for various diseases, including cancers. Due to their lengths, the specificity often becomes an issue in conventional amplification-based methods. Next-generation sequencing techniques could be alternative, but long analysis time expensive costs make them less suitable routine clinical diagnosis. Therefore, it is essential develop a rapid, selective, accurate miRNA detection assay using simple, affordable system. In this work, we report CRISPR/Cas13a-based biosensing point-of-care dark-field (DF) imaging. We utilized magnetic-gold nanoparticle (MGNPs) complexes signal probes, which consist of 200 nm-sized magnetic beads 60 gold nanoparticles (AuNPs) linked by DNA hybridization. Once CRISPR/Cas13a system recognized target miRNAs (miR-21-5p), activated Cas13a cleaved bridge linker containing RNA sequences, releasing nm-AuNPs detected quantified portable DF imaging The combination CRISPR/Cas13a, MGNPs, demonstrated amplification-free miR-21-5p within 30 min at limit 500 attomoles (25 pM) with single-base specificity. CRISPR/Cas13a-assisted MGNP-DF achieved simple equipment, thus providing application cancer

Language: Английский

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