Journal of Translational Medicine,
Journal Year:
2024,
Volume and Issue:
22(1)
Published: Dec. 20, 2024
Stem
cells
derived
from
the
apical
papilla
(SCAPs)
play
a
crucial
role
in
tooth
root
development
and
dental
pulp
regeneration.
They
are
important
seed
for
bone/tooth
tissue
engineering.
However,
replicative
senescence
remains
an
unavoidable
issue
as
vitro
amplification
increases.
This
study
investigated
effect
of
three-dimensional
(3D)
culture
environment
constructed
with
methylcellulose
on
SCAPs
senescence.
It
was
observed
that
3D
conditions
can
delay
cellular
senescence,
potentially
due
to
changes
mitochondrial
function
oxidative
phosphorylation.
Transcriptome
high-throughput
sequencing
technology
revealed
different
states
may
be
related
UQCRC2.
Knocking
down
UQCRC2
expression
group
resulted
increased
production
reactive
oxygen
species,
decreased
membrane
potential,
decline
consumption
rate
phosphorylation,
accelerating
cell
The
results
this
indicated
mitigate
aging
by
maintaining
UQCRC2-mediated
homeostasis.
These
findings
provide
new
solution
during
promote
applications
SCAPs-based
clinical
translation.
Journal of the American Chemical Society,
Journal Year:
2024,
Volume and Issue:
146(19), P. 12941 - 12949
Published: April 30, 2024
Nucleic
acids
are
mainly
found
in
the
mitochondria
and
nuclei
of
cells.
Detecting
nucleic
mitochondrion
nucleus
cascade
mode
is
crucial
for
understanding
diverse
biological
processes.
This
study
introduces
a
novel
acid-based
fluorescent
styrene
dye
(SPP)
that
exhibits
light-driven
migration
from
to
nucleus.
By
introducing
N-arylpyridine
on
one
side
skeleton
bis(2-ethylsulfanyl-ethy)-amino
unit
other
side,
we
SPP
excellent
DNA
specificity
(16-fold,
FDNA/Ffree)
stronger
binding
force
nuclear
(−5.09
kcal/mol)
than
mitochondrial
(−2.59
kcal/mol).
initially
accumulates
then
migrates
within
10
s
under
light
irradiation.
tracking
damage
apoptotic
cells,
allows
successful
visualization
differences
between
apoptosis
ferroptosis.
Finally,
triphenylamine
segment
with
photodynamic
effects
was
incorporated
into
form
photosensitizer
(MTPA-SPP),
which
targets
photosensitization
irradiation
enhanced
cancer
cell
treatment.
innovative
molecule
light-triggered
mitochondrion-to-nucleus
ability
provides
feasible
approach
situ
identification
acids,
monitoring
subcellular
physiological
events,
efficient
therapy.
Advanced Materials,
Journal Year:
2023,
Volume and Issue:
unknown
Published: Sept. 4, 2023
Neoadjuvant
and
adjuvant
therapies
have
made
significant
progress
in
cancer
treatment.
However,
tumor
therapy
still
faces
challenges
due
to
the
intrinsic
heterogeneity
of
cancer,
genomic
instability,
formation
an
immunosuppressive
microenvironment.
Functional
materials
possess
unique
biological
properties
such
as
long
circulation
times,
tumor-specific
targeting,
immunomodulation.
The
combination
functional
with
natural
substances
nanotechnology
has
led
development
smart
biomaterials
multiple
functions,
high
biocompatibilities,
negligible
immunogenicities,
which
can
be
used
for
precise
Recently,
subcellular
structure-targeting
received
particular
attention
various
biomedical
applications
including
diagnosis,
sensing,
imaging
tumors
drug
delivery.
Subcellular
organelle-targeting
precisely
accumulate
therapeutic
agents
organelles,
considerably
reduce
threshold
dosages
agents,
minimize
drug-related
side
effects.
This
review
provides
a
systematic
comprehensive
overview
research
organelle-targeted
based
on
nanomaterials.
Moreover,
it
explains
prospects
precision
oncology.
will
serve
excellent
cutting-edge
guide
researchers
field
therapy.
Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
97(1), P. 1003 - 1009
Published: Jan. 1, 2025
At
present,
some
progress
has
been
made
in
developing
NIR
light-responsive
free
radical
generators.
However,
the
efficacy
of
theranostics
continues
to
be
hindered
by
tumor-associated
inflammatory
reactions.
Hence,
fulfilling
situ
release
radicals
upon
light
excitation
specifically
activated
inflammation
microenvironment
would
an
ideal
strategy
for
efficient
eradication
and
tumor
suppression
but
remains
a
challenge.
Herein,
SO2
(overexpressed
reactive
sulfur
species
inflamed
site)-stimulated
phototheranostic
agent
(CVS)
is
successfully
developed.
Through
specific
response
both
endogenous
exogenous
with
low
LOD
(31.7
nM),
CVS
demonstrates
"switch
on"
two-photon
activity
as
well
OH·
generation.
Remarkably,
CVS-treated
H22-tumor-bearing
mice,
light-activated
accurate
are
accomplished.
This
platform
not
only
facilitates
quantification
during
also
renders
it
potent
antihypoxic
agent.
The Analyst,
Journal Year:
2023,
Volume and Issue:
148(17), P. 4174 - 4179
Published: Jan. 1, 2023
A
dual-response
fluorescent
probe
(MBI)
was
developed
for
the
detection
of
SO
2
and
viscosity.
Probe
MBI
successfully
applied
to
image
viscosity
during
mitochondrial
dysfunction
cell
apoptosis
with
good
performance.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(16), P. 6493 - 6500
Published: April 10, 2024
Mitochondria
play
a
crucial
role
in
maintaining
cellular
homeostasis,
and
the
depolarization
of
mitochondrial
membrane
potential
(MMP)
is
an
important
signal
apoptosis.
Additionally,
protein
misfolding
aggregation
are
closely
related
to
diseases
including
neurodegenerative
diseases,
diabetes,
cancers.
However,
interaction
between
MMP
changes
disease-related
was
rarely
studied.
Herein,
we
report
novel
"turn-on"
fluorescent
probe
MitoRhB
that
specifically
targets
mitochondria
for
Cu2+
detection
situ.
The
fluorescence
lifetime
(τ)
exhibits
positive
correlation
with
changes,
allowing
us
quantitatively
determine
relative
during
SOD1
(A4
V)
aggregation.
Finally,
found
(1)
increasing
concentrations
copper
will
accelerate
reduce
MMP;
(2)
can
intensify
degree
aggregation,
suggesting
new
routine
copper-induced
cell
death
mediated
through
abnormal
