Tris(2-carboxyethyl)phosphine-mediated immobilization of thiolated DNA on gold nanoclusters and its application in multiplex microRNA detection DOI
Weidong Qin, Junhua Wang, Zhanqiu Tang

et al.

Sensors and Actuators B Chemical, Journal Year: 2023, Volume and Issue: 401, P. 135028 - 135028

Published: Nov. 23, 2023

Language: Английский

Advances in DNA walking nanomachine‐based biosensors DOI Creative Commons
Bo Shen, Li Li, Minghui Guo

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 2(1)

Published: Jan. 1, 2024

Abstract The field of DNA nanotechnology has evolved beyond the realm controllable movements and randomly shaped nanostructures, now encompassing a diverse array nanomachines, each with unique nanostructures biofunctional attributes. These boast exceptional characteristics such as programmability, integrability, biocompatibility, universality. Among this variety, walking nanomachines have emerged one most prominent nanomotors, distinguished by their ingenious design comprehensive functionality. In recent times, these walkers witnessed remarkable advancements in areas ranging from nanostructural designs to biological applications, including creation sophisticated biosensors capable efficiently detecting tumor‐related biomarkers bioactive substances. This review delves into operational mechanisms which are driven processes protease DNAzyme action well strand displacement photoactivated reactions. It further provides overview different dimensional (1D, 2D, 3D) tracks. A subsequent section introduces biosensing applications electrochemical, optical, other biosensors. concludes forward‐looking perspective on novel challenges developing nanomachine‐based

Language: Английский

Citations

14
Split activator of CRISPR/Cas12a for direct and sensitive detection of microRNA DOI

Wen He,

Xinyu Li, Xinmin Li

et al.

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1303, P. 342477 - 342477

Published: March 22, 2024

Language: Английский

Citations

13
A novel electrochemiluminescent cytosensor using 3D multivalent aptamer recognition and covalent organic frameworks-associated DNA walker for highly efficient capture and detection of rare CTCs DOI
Minghui Guo, Bo Shen, Wen He

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 481, P. 148606 - 148606

Published: Jan. 6, 2024

Language: Английский

Citations

10
Enhancing 3D DNA Walker-Induced CRISPR/Cas12a Technology for Highly Sensitive Detection of ExomicroRNA Associated with Osteoporosis DOI
Lijuan Luo, Fang Dong, Dandan Li

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(3), P. 1438 - 1446

Published: March 7, 2024

Exosomal microRNAs (exomiRNAs) have emerged as promising biomarkers for the early clinical diagnosis of osteoporosis. However, their limited abundance and short length in peripheral blood present significant challenges accurate detection exomiRNAs. Herein, we designed implemented an efficacious fluorescence-based biosensor highly sensitive exomiRNA associated with osteoporosis, leveraging enhancing 3D DNA walker-induced CRISPR/Cas12a technology. The engineered walker is capable efficiently transforming target into amplifying strands, thereby sensitivity developed biosensor. Concurrently, liberated strands serve activators to trigger Cas12a trans-cleavage activity, culminating a significantly amplified fluorescent signal exomiRNA-214. Under optimal conditions, devised technology demonstrated capacity detect exomiRNA-214 at concentrations low 20.42 fM, encompassing wide linear range extending from 50.0 fM 10.0 nM. Moreover, could accurately differentiate between healthy individuals osteoporosis patients via exomiRNA-214, which was agreement RT-qPCR results. As such, this biosensing offers promise valuable tool

Language: Английский

Citations

9
Comprehensive insights into pathogenicity and emerging CRISPR-integrated biosensors for the early detection of breast cancer biomarkers DOI

Ashok Saini,

Neeraj Dilbaghi, Neelam Yadav

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 112908 - 112908

Published: Jan. 1, 2025

Language: Английский

Citations

1
Tetrahedral DNA frameworks for biosensing and imaging analysis in living cells DOI
Zhijie Qi,

Chen Wei,

Fei Zhang

et al.

Nano Today, Journal Year: 2023, Volume and Issue: 54, P. 102127 - 102127

Published: Dec. 30, 2023

Language: Английский

Citations

21
Antibody-Protein-Aptamer Electrochemical Biosensor based on Highly Efficient Proximity-Induced DNA Hybridization on Tetrahedral DNA Nanostructure for Sensitive Detection of Insulin-like Growth Factor-1 DOI

Lin-Lin Long,

Hu W, Xin Wang

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(9), P. 3837 - 3843

Published: Feb. 22, 2024

Herein, an antibody-protein-aptamer electrochemical biosensor was designed by highly efficient proximity-induced DNA hybridization on a tetrahedral nanostructure (TDN) for ultrasensitive detection of human insulin-like growth factor-1 (IGF-1). Impressively, the IGF-1 antibody immobilized top vertex TDN could effectively capture target protein with less steric effect, and ferrocene-labeled signal probe (SP) bound bottom close to electrode surface generating strong initial signal. In presence aptamer strand, sandwich be formed TDN, which would trigger release SP TDN; therefore, response decrease dramatically, enhancing sensitivity biosensor. As result, linear range proposed 1 fM nM limit down 0.47 fM, much lower than that traditional designs biosensors. Surprisingly, use this approach offered innovative sensitive biomarkers illness diagnosis.

Language: Английский

Citations

6
Nanozyme-based cancer theranostics: A scientometric analysis and comprehensive review DOI
Xing Yang, Feroza Yasinjan,

Shuhao Sun

et al.

Nano Today, Journal Year: 2024, Volume and Issue: 57, P. 102386 - 102386

Published: July 13, 2024

Language: Английский

Citations

6
A Comprehensive Review on Electrochemical Nano Biosensors for Precise Detection of Blood-Based Oncomarkers in Breast Cancer DOI Creative Commons
Mahdi Sadeghi, Somayeh Sadeghi, Seyed Morteza Naghib

et al.

Biosensors, Journal Year: 2023, Volume and Issue: 13(4), P. 481 - 481

Published: April 16, 2023

Breast cancer (BC), one of the most common and life-threatening cancers, has highest incidence rate among women. Early diagnosis BC oncomarkers is considered effective strategy for detecting treating BC. Finding type stage in women as soon possible greatest ways to stop its negative effects on medical treatment. The development biosensors early, sensitive, selective detection recently attracted much attention. An electrochemical nano biosensor (EN) a very suitable option powerful tool diagnosis. This comprehensive review provides information about prevalence pathobiology BC, recent advances clinically available oncomarkers, point-of-care (POC) various using nanomaterial-based signal amplification techniques.

Language: Английский

Citations

15
Next-generation CRISPR-based diagnostic tools for human diseases DOI
Ting Wang, Ziwei Wang,

Linlin Bai

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 168, P. 117328 - 117328

Published: Sept. 25, 2023

Language: Английский

Citations

13