Rapid and Visual Detection of Urinary Pathogens by Employing Bifunctional Deoxyribonucleic Acid Sensors and Aggregation of Gold Nanoparticles DOI

Hanren Chen,

Xiumei Hu, Jianbo Xiao

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 26, 2024

A simple, rapid, and visual approach is developed to perform diagnosis of urinary tract infection (UTI) antimicrobial susceptibility testing (AST) by employing smart bifunctional DNA (bfDNA) sensors, exonuclease III, concatermers CuO nanoparticles (CuONPs), gold NPs (AuNPs) aggregation [AuNPs agglutination (AA)], namely, the bfDEC-AA method. The bfDNA sensors serve as probes for identifying 16S rRNA genes bacterium or 18S fungus mediators connecting CuONPs. AA a signal source triggered Cu(I)-catalyzed azide–alkyne cycloaddition click chemistry. In this study, urine samples are analyzed directly, eliminating need overnight incubation bacterial isolation. crucial color change from red purple occurs at UTI diagnostic threshold 104 CFU mL–1; thus results can be qualitatively interpreted unaided eyes. Similarly, AST was performed visually under our optimal conditions. shift also quantified using point-of-care (POC) device. identification, bacteria strain were achieved within 55, 145 min 96 samples, respectively. Here, 128 suspected patients tested. Notably, sensitivity specificity method 99% 100% Gram-negative (G–) infection, Gram-positive (G+) 98% AST, Furthermore, low cost POC device (US$156) friendly underdeveloped regions.

Language: Английский

Research progress on preparation and fluorescence sensing performance of layered double hydroxides-based inorganic/organic luminescent nanocomposites DOI
Shufang Zheng, Yuyin Wang, Mengyao Li

et al.

Materials Today Communications, Journal Year: 2024, Volume and Issue: 38, P. 108466 - 108466

Published: Feb. 22, 2024

Language: Английский

Citations

4
Rapid Detection of Broad-Spectrum Pathogenic Bacteria Based on Highly Sensitive Proton Response of the Nucleic Acid Amplification SPQC Platform DOI

Yusheng Liao,

Yu Liu, Ye Feng

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(17), P. 6756 - 6763

Published: April 16, 2024

Pathogenic bacteria significantly contribute to elevated morbidity and mortality rates, highlighting the urgent need for early precise detection. Currently, there is a paucity of effective broad-spectrum methods detecting pathogenic bacteria. We have developed an innovative proton-responsive series piezoelectric quartz crystal (PR-SPQC) platform identification This was achieved by retrieving aligning sequences from NCBI GenBank database identify validate 16S rRNA oligonucleotide that are signatures but absent in humans or fungi. The hyperbranched rolling circle amplification, activated exclusively screened target, exponentially generates protons detected SPQC through 2D polyaniline (PANI) film. PR-SPQC demonstrates capabilities bacteria, with detection limit 2 CFU/mL within 90 min. Clinical testing blood samples yielded satisfactory results. With its advantages miniaturization, cost efficiency, suitability point-of-care testing, has potential be extensively used rapid diverse clinical practice public health sectors.

Language: Английский

Citations

4
Exonuclease III/Cas12a Cascade Amplification Strategy and Smartphone-Based Portable Fluorescence Detector to Repurpose the Commercial AFP Strip for the POCT of Multiple RNAs DOI
Xin Peng, Xuecui Mei, Xueyan Liu

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(32), P. 13252 - 13259

Published: July 31, 2024

Point of care testing (POCT) nucleic acid (NA) contributes to the timely disease diagnosis, like bacteria and virus screening in households or resource-constrained areas, but its development has always been stagnant. Herein, we proposed an exonuclease III cascaded with CRISPR/Cas12a (Exo-III/Cas12a) amplification strategy constructed a smartphone-based portable fluorescence detector (SPFD) repurpose commercial alpha-fetoprotein (AFP) strip for ultrasensitive hand-held detection NA samples. In detail, target-initiated-Exo-III/Cas12a realizes signal liberates AFP from magnetic beads through trans-cleavages activated Cas12a toward aptamer. After separation migration, signals test (FT) control (FC) lines on were digitally output by SPFD, FT/FC was employed quantitative analysis minimize external disturbances improve accuracy. We experimentally assessed universe applicability NA-POCT platform miRNA-155, 16S rRNA Staphylococcus aureus, ORF1a/b RNA Covid-19 pseudovirus, achieving favorable limits 42 aM, 18 CFU/mL, 87 copies/μL, respectively. Moreover, simplicity, universality, admirable performance demonstrate great potential aspect rapidly transforming existing POCT devices multiple new applications at time need.

Language: Английский

Citations

4
Metal and metal oxide nanoparticle-assisted molecular assays for the detection of Salmonella DOI Creative Commons
Linlin Zhuang, Jiansen Gong, Di Zhang

et al.

Discover Nano, Journal Year: 2025, Volume and Issue: 20(1)

Published: April 2, 2025

This paper provides a comprehensive overview of the diverse applications and innovations nanoparticles in detection Salmonella. It encompasses range novel methods, including efficient enrichment, nucleic acid extraction, immunoassays, tests, biosensors, emerging strategies with potential for future applications. The surface modification specific antibodies or ligands enables to achieve highly selective capture Salmonella, while optimizing extraction process improving efficiency. employment immunological tests markedly enhances specificity sensitivity reaction, thereby determination results. Moreover, distinctive physicochemical properties enhance sensitivity, selectivity, stability facilitating rapid advancement bio-detection technologies. is particularly noteworthy that there has been significant application innovative research nanozymes molecular assays. progress not only resulted enhanced efficiency but also facilitated innovation improvement As nanotechnologies continue advance, use metal oxide Salmonella likely become more promising reliable strategy ensuring food safety public health.

Language: Английский

Citations

0
Dual-sensitized heterojunction Ag2S/ZnS/NiS composites with entire visible-light region absorption for ultrasensitive photoelectrochemical detection of tobramycin DOI

Yushuang Jin,

Wanqing Yu, Qiang Chen

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 260, P. 116459 - 116459

Published: June 1, 2024

Language: Английский

Citations

3
Biomedical Utility of Non‐Enzymatic DNA Amplification Reaction: From Material Design to Diagnosis and Treatment DOI
Ye Chen, Zhiqiang Liu, Bowen Zhang

et al.

Small, Journal Year: 2024, Volume and Issue: 20(47)

Published: Aug. 17, 2024

Nucleic acid nanotechnology has become a promising strategy for disease diagnosis and treatment, owing to remarkable programmability, precision, biocompatibility. However, current biosensing biotherapy approaches by nucleic acids exhibit limitations in sensitivity, specificity, versatility, real-time monitoring. DNA amplification reactions present an advantageous enhance the performance of platforms. Non-enzymatic reaction (NEDAR), such as hybridization chain catalytic hairpin assembly, operate via strand displacement. NEDAR presents distinct advantages over traditional enzymatic reactions, including simplified procedures, milder conditions, higher enhanced controllability, excellent versatility. Consequently, research focusing on NEDAR-based garnered significant attention. demonstrates high efficacy detecting multiple types biomarkers, acids, small molecules, proteins, with sensitivity enabling parallel detection targets. Besides, can strengthen drug therapy, cellular behavior control, cell encapsulation. Moreover, holds promise constructing assembled diagnosis-treatment nanoplatforms forms pure nanostructures hybrid nanomaterials, which offer utility monitoring precise treatment. Thus, this paper aims comprehensively elucidate mechanism review substantial advancements treatment past five years, encompassing design strategies, applications, prospects.

Language: Английский

Citations

3
Lateral flow strip assay of a gene segment in the COVID-19 virus with combined dual readout mode and preliminary multisite hybrid chain reaction amplification DOI
Shiyong Yu,

Daoguangyao Zhang,

Hao Jiang

et al.

Analytical Methods, Journal Year: 2024, Volume and Issue: 16(20), P. 3249 - 3255

Published: Jan. 1, 2024

The past and present scenario of COVID-19 has revealed the necessity simple point-of-care tests. When combined with great advantages amplification, lateral flow assay nucleic acid analysis represents a more sensitive molecular diagnostic technique compared to universal protein analysis. Room temperature operation, an enzyme-free nature,

Language: Английский

Citations

1
Enzyme-Assisted Solid-Phase Microextraction Coupled with a DNA Nanowalker for Dual-Amplified Detection of Chloramphenicol in Animal-Derived Food Products DOI

Yitong Chen,

Zhuoyue Shen,

Chunxu Tao

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 21, 2024

Chloramphenicol (CAP), an aminoalcohol antibiotic, exerts its action on bacterial ribosomes, thereby obstructing protein synthesis. However, the use of CAP in husbandry may lead to excessive accumulation animal-derived food products. This presents potential risks consumer health. study developed a novel dual-amplification fluorescence detection method by integrating enzyme-assisted solid-phase microextraction (SPME) with Fe3O4@Au NP-based DNA nanowalker for food. The combination quartz rod-based SPME biosensor and effectively eliminated matrix interference, enabling conversion enhancement signals through two cyclic amplification processes. strategy demonstrated high sensitivity limit 28.1 aM as well wide linear range from 0.1 fM 1 nM (with R2 > 0.99). also demonstrates robust stability accuracy detecting trace amounts both authentic prepared positive samples.

Language: Английский

Citations

1
Short-stranded DNA segment regulates alterable LAMP(H+) to switch pH-responsive intramolecular hairpins for amplified electrochemical biosensing DOI

Yifu Zhou,

Jiayi Fu,

Wu Xu

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 420, P. 136385 - 136385

Published: Aug. 3, 2024

Language: Английский

Citations

0
Barcoding of Life for Detection and Diagnosis of Diseases and Pests in Potato DOI
Sarika Yadav, Neelam Yadav, Satyendra Mohan Paul Khurana

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0