Iridium Isotope Tag-Assisted LC-MS Method for Global Profiling and Quantification of Nonvolatile Serum Fatty Acids in Nonalcoholic Fatty Liver Mice

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Highly accurate and sensitive measurements of fatty acids (FAs) in biological samples are essential for advancing our understanding their diverse biofunctions. In this work, based on the characteristic isotope pattern iridium (191/193Ir), we employed an iridium-encoded amine (Ir-NH2) as derivatization reagent to establish a selective liquid chromatography–mass spectrometry (LC-MS) method rapid identification quantification FAs samples. Upon derivatization, nonvolatile were transformed into amide derivatives tagged with charged tag, exhibiting improved sensitivity selectivity electrospray ionization (ESI) positive ion mode. By leveraging unique 2.002 Da mass shift 3:5 peak intensity ratio from natural 191Ir 193Ir isotopes, can rapidly efficiently screen potential carboxyl-containing metabolites Compared other existing methods, technique offers higher sensitivity, better signal-to-noise ratio, lower detection limit (1.2–8.4 pg/mL), easier due clear iridium-tagged derivatives. With method, total 58 FAs, including both saturated unsaturated types, detected mice serum lipid extracts, carbon chain lengths varying C9 C24. More importantly, was successfully global profiling nonalcoholic liver disease (NAFLD), providing novel means detecting them offering new avenues exploring functional roles associations.

Language: Английский

Enhanced Breath Aldehyde Analysis by Dual-Membrane-Assisted Charge Tagging, Enrichment, and Onsite Elution NanoESI-MS

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Aldehydes, crucial volatile organic compounds present in exhaled breath, have been established as promising biomarkers for cancer diagnosis. However, their rapid and sensitive detection through widely employed spray-based ionization mass spectrometry is still challenging. To address this, we introduce a charged "iridium isotopic signature" probe tailored efficient capture unambiguous identification of ubiquitous aldehydes the gas phase. This 191/193Ir-tagged spectrometric probe, equipped with reactive amine moiety capable interacting aldehydes, immobilized on porous Nylon-6 membrane that facilitates transport enriches from complex breath matrix. Following solvent extraction, Ir-tagging aldehyde derivatives were successfully eluted removal excess probes by oxidized cellulose membrane, yielding purified sample ideally suited direct, rapid, ultrasensitive (with limit below 0.1 ppt) nanoelectrospray (nanoESI-MS) analysis. By utilizing an analogous iridium internal standard, our method precisely identified quantified 12 (EB), several exhibiting significant elevations esophageal patients compared healthy controls. highlights its efficacy accurate tool detecting biomarkers, offering avenues

Language: Английский

Bromine signature coded derivatization LC-MS for specific profiling of carboxyl or carbonyl-containing metabolites in Mycoplasma pneumoniae infection

Talanta, Journal Year: 2024, Volume and Issue: 285, P. 127345 - 127345

Published: Dec. 6, 2024

Language: Английский