Identification of Plant Compounds from Mass Spectrometry Imaging (MSI) DOI Open Access
Nancy Shyrley García‐Rojas, Carlos Daniel Sierra-Álvarez, Hilda Ramos-Aboites

et al.

Published: June 25, 2024

The presence and localization of plant metabolites are indicative physiological processes, e.g., under biotic abiotic stress conditions. Further, the chemical composition parts is related to their quality as food or for medicinal applications. Mass spectrometry imaging (MSI) has become a popular analytical technique exploring visualizing spatial distribution molecules within tissue. This review provides summary mass methods used mapping identifying in tissues. We present benefits disadvantages both vacuum ambient ionization methods, considering direct indirect approaches. Finally, we discuss current limitations annotating perspectives future investigations.

Language: Английский

Identification of Plant Compounds with Mass Spectrometry Imaging (MSI) DOI Creative Commons
Nancy Shyrley García‐Rojas, Carlos Daniel Sierra-Álvarez, Hilda Ramos-Aboites

et al.

Metabolites, Journal Year: 2024, Volume and Issue: 14(8), P. 419 - 419

Published: July 30, 2024

The presence and localization of plant metabolites are indicative physiological processes, e.g., under biotic abiotic stress conditions. Further, the chemical composition parts is related to their quality as food or for medicinal applications. Mass spectrometry imaging (MSI) has become a popular analytical technique exploring visualizing spatial distribution molecules within tissue. This review provides summary mass methods used mapping identifying in tissues. We present benefits disadvantages both vacuum ambient ionization methods, considering direct indirect approaches. Finally, we discuss current limitations annotating perspectives future investigations.

Language: Английский

Citations

2
RhizoMAP: a comprehensive, nondestructive, and sensitive platform for metabolic imaging of the rhizosphere DOI Creative Commons
Dušan Veličković,

Tanya Winkler,

Vimal Kumar Balasubramanian

et al.

Plant Methods, Journal Year: 2024, Volume and Issue: 20(1)

Published: Aug. 2, 2024

Elucidating the intricate structural organization and spatial gradients of biomolecular composition within rhizosphere is critical to understanding important biogeochemical processes, which include mechanisms root-microbe interactions for maintaining sustainable plant ecosystem services. While various analytical methods have been developed assess heterogeneity rhizosphere, a comprehensive view fine distribution metabolites root-soil interface has remained significant challenge. This primarily due difficulty original during sample preparation without compromising its molecular content. In this study, we present novel approach, RhizoMAP, in molecules are imprinted on selected polymer membranes then spatially profiled using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI). We enhanced performance RhizoMAP by combining use two thin (< 20 μm) (polyester polycarbonate) with distinct MALDI preparations. optimization allowed us gain insight into over 500 different poplar (Populus trichocarpa) grown rhizoboxes filled mycorrhizae soil. These membranes, coupled three conditions, enabled capture wide variety that included phytohormones, amino acids, sugars, sugar glycosides, polycarboxylic acids components Krebs cycle, fatty short aldehydes ketones, terpenes, volatile organic compounds, fertilizers from soil, others. Their varies greatly, some following root traces, others showing diffusion roots, associated soil particles, many having hot spots along or surrounding Moreover, showed how can be used localize origin transformation growth. Finally, demonstrated power distributions key throughout cm deep rhizosphere. method provides nondestructive, untargeted, broad, sensitive metabolite root-associated molecules, exudates, matter as lab-controlled native environment.

Language: Английский

Citations

2
Imaging the spatial distribution of structurally diverse plant hormones: A comprehensive review DOI
Lulu Chen, Yue Zhang,

Yufen Bu

et al.

Journal of Experimental Botany, Journal Year: 2024, Volume and Issue: 75(22), P. 6980 - 6997

Published: Sept. 12, 2024

Abstract Plant hormones are essential and structurally diverse molecules that regulate various aspects of plant growth, development, stress responses. However, the precise analysis in complex biological samples poses a challenge due to their low concentrations, dynamic levels, intricate spatial distribution. Moreover, complexity interconnectedness hormone signaling networks make it difficult simultaneously trace multiple distributions. In this review, we provide an overview currently recognized small-molecule hormones, signal peptide growth regulators, along with analytical methods employed for analysis. We delve into latest advancements mass spectrometry imaging situ fluorescence techniques, which enable examination distribution hormones. The advantages disadvantages these techniques further discussed. Finally, propose potential avenues enhance our understanding biology.

Language: Английский

Citations

2
Experimental and Computational Evaluation of Lipidomic In-Source Fragmentation as a Result of Postionization with Matrix-Assisted Laser Desorption/Ionization DOI
Gregory W. Vandergrift, William Kew, Amity Andersen

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 19, 2024

Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) can provide spatially resolved molecular information about a sample. Recently, postionization approach (MALDI-2) has been commercially integrated with MALDI-MSI, allowing for bettered sensitivity and consequent improved spatial resolution. While advantages of MALDI-2 have previously established, we demonstrate here statistically increased in-source fragmentation (ISF) results from commercial instrument. Via lipid standard analyses, known MALDI ISF pathways (e.g., loss trimethylamine) were in compared to MALDI-1 (65-172% increase fragmentation). Gas phase modeling density functional theory estimated that the most-weighted virtual orbitals excite within lipids involve ester phosphate bonds. Protonated excitation energies are furthermore red-shifted those other adduct types [e.g., 254 nm protonated PC(16:0/18:1)] energy (266 nm). Analysis rat brain homogenate detected more positive-ion mode peaks (1090) than (719), where Kernel estimations showed majority this enhancement occurs low

Language: Английский

Citations

2
Mass spectrometry imaging of natural carbonyl products directly from agar-based microbial interactions using 4-APEBA derivatization DOI Creative Commons
Dušan Veličković, Kevin Zemaitis, Arunima Bhattacharjee

et al.

mSystems, Journal Year: 2023, Volume and Issue: 9(1)

Published: Dec. 8, 2023

Aliphatic carboxylic acids, aldehydes, and ketones play diverse roles in microbial adaptation to their microenvironment, from excretion as toxins adaptive metabolites for membrane fluidity. However, the spatial distribution of these molecules throughout biofilms how microbes environments exchange remain elusive many bioactive species due inefficient molecular imaging strategies. Herein, we apply on-tissue chemical derivatization (OTCD) using 4-(2-((4-bromophenethyl)dimethylammonio)ethoxy)benzenaminium dibromide (4-APEBA) on a co-culture soil bacterium (

Language: Английский

Citations

5
A selective process for application of EDC and 4-APEBA for carbonyl containing metabolites by MALDI-MSI v2 DOI Open Access
Kevin Zemaitis, Christopher Anderton, Dušan Veličković

et al.

Published: June 10, 2024

Herein, we outline the protocol for application of 4-(2-((4-bromophenethyl)dimethylammonium)ethoxy)benzenaminium dibromide (4-APEBA) which have previously demonstrated to be an effect reagent in tandem with 1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide (EDC) target carbonyl containing metabolites in-situ matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). Either or without selective EDC, MALDI-MSI is able differentiate ketones and aldehydes from carboxylic acids lending more confidence annotations. This has enabled metabolic pathway analyses high-levels coverage routinely enabling detect over a hundred carbonyls. was previous introduced LC-MS/MS purposes (see references), but generally through our on-tissue/on-target chemical derivatization (OTCD) find that (1) increased sensitivity primary metabolites, (2) within annotations due bromine isotopic patterns, (3) do not delocalize process OTCD, (4) multiplexed detection non-derivatized lipids OTCD products, (5) this applicable broad variety mammalian plant tissues, microbial cultures.

Language: Английский

Citations

1
Spatiotemporal Metabolic Responses to Water Deficit Stress in Distinct Leaf Cell-types of Poplar DOI Creative Commons

Vimal Kumar Balasubramanian,

Dušan Veličković, María del Mar Rubio Wilhelmi

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 1, 2023

Abstract The impact of water-deficit (WD) stress on plant metabolism has been predominantly studied at the whole tissue level. However, tissues are made several distinct cell types with unique and differentiated functions, which limits ‘omics’-based studies to determine only an averaged molecular signature arising from multiple types. Advancements in spatial omics technologies provide opportunity understand mechanisms underlying responses WD cell-type levels. Here, we spatiotemporal metabolic two poplar leaf types-palisade vascular cells-to using matrix-assisted laser desorption Ionization-mass spectrometry imaging (MALDI-MSI). We identified stress-mediated shifts each type when exposed early prolonged recovery stress. During stress, flavonoids phenolic metabolites were exclusively accumulated palisade cells. cells mainly sugars during fatty acids conditions, highlighting a possibility interconversion between under conditions By comparing our MALDI-MSI data gas chromatography-mass (GC-MS)-based profile, few that showed similar accumulation trend both Overall, this work highlights potential MSI approach complement tissue-based metabolomics techniques provides novel understanding This will help engineer specific pathways cellular level strategic perennial trees like poplars withstand future aberrations environmental increase bioenergy sustainability.

Language: Английский

Citations

2
A selective process for application of EDC and 4-APEBA for cabonyl containing metabolites by MALDI-MSI v1 DOI Open Access
Kevin Zemaitis, Christopher Anderton, Dušan Veličković

et al.

Published: June 10, 2024

Herein, we outline the protocol for application of 4-(2-((4-bromophenethyl)dimethylammonium)ethoxy)benzenaminium dibromide (4-APEBA) which have previously demonstrated to be an effect reagent in tandem with 1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide (EDC) target carbonyl containing metabolites in-situ matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). Either or without selective EDC, MALDI-MSI is able differentiate ketones and aldehydes from carboxylic acids lending more confidence annotations. This has enabled metabolic pathway analyses high-levels coverage routinely enabling detect over a hundred carbonyls. was previous introduced LC-MS/MS purposes (see references), but generally through our on-tissue/on-target chemical derivatization (OTCD) find that (1) increased sensitivity primary metabolites, (2) within annotations due bromine isotopic patterns, (3) do not delocalize process OTCD, (4) multiplexed detection non-derivatized lipids OTCD products, (5) this applicable broad variety mammalian plant tissues, microbial cultures.

Language: Английский

Citations

0
Identification of Plant Compounds from Mass Spectrometry Imaging (MSI) DOI Open Access
Nancy Shyrley García‐Rojas, Carlos Daniel Sierra-Álvarez, Hilda Ramos-Aboites

et al.

Published: June 25, 2024

The presence and localization of plant metabolites are indicative physiological processes, e.g., under biotic abiotic stress conditions. Further, the chemical composition parts is related to their quality as food or for medicinal applications. Mass spectrometry imaging (MSI) has become a popular analytical technique exploring visualizing spatial distribution molecules within tissue. This review provides summary mass methods used mapping identifying in tissues. We present benefits disadvantages both vacuum ambient ionization methods, considering direct indirect approaches. Finally, we discuss current limitations annotating perspectives future investigations.

Language: Английский

Citations

0