Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(29), P. 12012 - 12021
Published: July 8, 2024
The
development
of
liquid
biopsy
methods
for
the
accurate
and
reliable
detection
miRNAs
in
whole
blood
is
critical
early
diagnosis
monitoring
diseases.
However,
quantification
miRNA
expression
levels
remains
challenging
due
to
complex
matrix
low
abundance
samples.
Herein,
we
report
a
contactless
signal
output
strategy
with
background
interference
that
ensures
"zero-contact"
between
reaction
system
colorimetry
system.
designed
target-induced
magnetic
ZnS/ZIF-90/ZnS
network
can
serve
as
unique
amplifier
transducer.
It
releases
hydrogen
sulfide
(H
Small,
Journal Year:
2024,
Volume and Issue:
20(47)
Published: Aug. 17, 2024
Nucleic
acid
nanotechnology
has
become
a
promising
strategy
for
disease
diagnosis
and
treatment,
owing
to
remarkable
programmability,
precision,
biocompatibility.
However,
current
biosensing
biotherapy
approaches
by
nucleic
acids
exhibit
limitations
in
sensitivity,
specificity,
versatility,
real-time
monitoring.
DNA
amplification
reactions
present
an
advantageous
enhance
the
performance
of
platforms.
Non-enzymatic
reaction
(NEDAR),
such
as
hybridization
chain
catalytic
hairpin
assembly,
operate
via
strand
displacement.
NEDAR
presents
distinct
advantages
over
traditional
enzymatic
reactions,
including
simplified
procedures,
milder
conditions,
higher
enhanced
controllability,
excellent
versatility.
Consequently,
research
focusing
on
NEDAR-based
garnered
significant
attention.
demonstrates
high
efficacy
detecting
multiple
types
biomarkers,
acids,
small
molecules,
proteins,
with
sensitivity
enabling
parallel
detection
targets.
Besides,
can
strengthen
drug
therapy,
cellular
behavior
control,
cell
encapsulation.
Moreover,
holds
promise
constructing
assembled
diagnosis-treatment
nanoplatforms
forms
pure
nanostructures
hybrid
nanomaterials,
which
offer
utility
monitoring
precise
treatment.
Thus,
this
paper
aims
comprehensively
elucidate
mechanism
review
substantial
advancements
treatment
past
five
years,
encompassing
design
strategies,
applications,
prospects.
Journal of Materials Chemistry B,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Jan. 1, 2024
Nanostructures
integrating
pH-sensitive
DNA
motifs
have
emerged
as
versatile
platforms
for
active
tumor
targeting,
owing
to
their
ability
undergo
conformation
changes
in
response
the
common
acidic
environment
of
extracellular
matrix
and
endocytosis
pathway.
This
review
summarizes
latest
advances
design
application
various
pH-responsive
nanostructures
tumor-targeted
strategies,
including
recognition,
cell
imaging,
dynamic
nanocarrier
construction,
controlled
drug
release.
A
comprehensive
framework
pH-controlled
multi-stage
targeting
is
introduced,
addressing
divergences
strategies
intracellular
environments.
The
unique
attributes,
practical
performance
challenges
are
also
critically
discussed
provide
guidance
future
development
this
field.
Precision Chemistry,
Journal Year:
2025,
Volume and Issue:
3(4), P. 187 - 205
Published: March 3, 2025
Nonenzymatic
nucleic
acid
amplification
reactions,
especially
nonenzymatic
DNA
reactions
(NDARs),
are
thermodynamically
driven
processes
that
operate
without
enzymes,
relying
on
toehold-mediated
strand
displacement
(TMSD)
and
branch
migration.
With
their
sensitive
efficient
signal
capabilities,
NDARs
have
become
essential
tools
for
biomarker
detection
intracellular
imaging.
They
encompass
four
primary
methods:
catalytic
hairpin
assembly
(CHA),
hybridization
chain
reaction
(HCR),
DNAzyme-based
amplification,
entropy-driven
circuits
(EDC).
Based
mechanisms,
can
be
categorized
into
three
types:
stimuli-responsive
NDARs,
which
employ
single
strategies
triggered
by
specific
stimuli
like
pH,
light,
or
biomolecules;
cascade
integrate
two
more
stepwise
enhancement;
autocatalytic
achieve
exponential
through
self-sustained
cycling.
These
advanced
designs
progressively
improve
efficiency,
enhance
sensitivity,
minimize
background
noise,
enabling
precise
of
proteins,
viruses,
acids
as
well
applications
in
cancer
cell
imaging
therapy.
Compared
with
classical
these
approaches
significantly
reduce
leakage,
offering
broader
applicability
diagnostics,
imaging,
therapeutic
contexts.
This
review
summarizes
recent
advancements,
addresses
existing
challenges,
explores
future
directions,
providing
insights
the
development
NDARs.
