International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(24), P. 13706 - 13706
Published: Dec. 22, 2024
Single-cell
sequencing
technology
provides
apparent
advantages
in
cell
population
heterogeneity,
allowing
individuals
to
better
comprehend
tissues
and
organs.
Sequencing
is
currently
moving
beyond
the
standard
transcriptome
single-cell
level,
which
likely
bring
new
insights
into
function
of
breast
cells.
In
this
study,
we
examine
primary
types
involved
development,
as
well
achievements
study
scRNA-seq
microenvironment,
stressing
finding
novel
subsets
using
approaches
analyzing
problems
solutions
scRNA-seq.
Furthermore,
are
excited
about
field’s
promising
future.
<p><strong>The
theca,
a
highly
specialised
stromal
cell
layer
that
surrounds
ovarian
follicles,
first
appears
at
the
secondary
stage
of
follicle
development.
While
role
theca
in
folliculogenesis
is
well
understood,
spatiotemporal
dynamics
governing
differentiation
are
yet
to
be
fully
elucidated.
Spatial
transcriptomics
offers
unique
insight
into
molecular
processes
occur
during
by
capturing
mRNA
expression
patterns
within
tissues,
maintaining
their
spatial
context.
The
overall
objective
this
thesis
was
optimise
protocol
perform
mouse
tissue.
This
contributes
wider
project
which
aims
track
progenitor
differentiated
transition
situ
using
model.
I
refined
protocols
for
collecting
ovaries,
and
various
histological
techniques
(including
freezing,
embedding
cryosectioning)
prioritised
integrity
morphology
tissue
imaging,
while
still
allowing
recovery
high-quality
RNA
from
same
sample.
As
result,
able
validate
preparing
optimised
sample
preparation
protocol,
tissue-specific
permeabilization
optimisation
STOmics
Stereo-seq
Transcriptomics
kit,
contribute
final
transcriptomic
experiment.
prepared
fresh-frozen
block
containing
four
ovaries
mice
(n=4),
sufficient
quality
(RNA
Integrity
Number
(RIN)
7.4
±
0.1)
proceed
with
consistently
passed
all
DNA
concentration
fragment
size
control
checks
throughout
experiment,
indicating
successful
generating
high
(both
terms
quality)
used
prepare
RNA-seq
library.
serves
as
valuable
resource
future
studies
investigate
cellular
ovary.</strong></p>
Parasites & Vectors,
Journal Year:
2024,
Volume and Issue:
17(1)
Published: Dec. 18, 2024
Abstract
Background
Toxoplasma
gondii
is
an
obligate
protozoan
parasite
capable
of
infecting
a
wide
range
warm-blooded
animals
and
humans.
Current
treatment
options,
primarily
pyrimethamine
sulfadiazine,
have
limitations,
such
as
high
recurrence
rates,
long
durations,
limited
effectiveness
against
T.
.
There
unmet
need
for
novel,
safe,
low-toxicity,
highly
effective
treatments.
This
study
aimed
to
evaluate
the
anti-
effects
glabridin,
natural
compound
derived
from
roots
widely
used
medicinal
plant
Methods
The
cytotoxicity
glabridin
in
Vero
cells
was
assessed
using
CCK-8
cell
viability
assay.
Quantitative
polymerase
chain
reaction
(qPCR)
targeting
Tg-529
gene
developed
quantify
assess
inhibitory
on
proliferation.
Ultrastructural
changes
after
were
examined
electron
microscopy.
levels
reactive
oxygen
species
(ROS)
mitochondrial
membrane
potential
(ΔΨm)
ROS
ΔΨm
tachyzoites.
Additionally,
metabolomics
transcriptomics
analyses
conducted
investigate
mechanisms
underlying
glabridin’s
effects.
Results
Glabridin
exhibited
low
toxicity
host
effectively
inhibited
invasion
proliferation
vitro
time-dependent
manner.
Glabridin-treated
tachyzoites
significant
structural
alterations,
along
with
increased
production
reduction
ΔΨm.
Metabolomic
analysis
indicated
that
significantly
affected
amino
acid
metabolism
pathways
In
vivo,
improved
survival
rates
-infected
BALB/c
mice
at
dosage
100
mg/kg.
Conclusions
demonstrates
has
potent
likely
through
disruption
parasite.
These
findings
highlight
promising
therapeutic
agent
toxoplasmosis.
Graphical
abstract
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(24), P. 13706 - 13706
Published: Dec. 22, 2024
Single-cell
sequencing
technology
provides
apparent
advantages
in
cell
population
heterogeneity,
allowing
individuals
to
better
comprehend
tissues
and
organs.
Sequencing
is
currently
moving
beyond
the
standard
transcriptome
single-cell
level,
which
likely
bring
new
insights
into
function
of
breast
cells.
In
this
study,
we
examine
primary
types
involved
development,
as
well
achievements
study
scRNA-seq
microenvironment,
stressing
finding
novel
subsets
using
approaches
analyzing
problems
solutions
scRNA-seq.
Furthermore,
are
excited
about
field’s
promising
future.
