Research on the Flow Characteristics and Reaction Mechanisms of Lateral Flow Immunoassay under Non-Uniform Flow DOI Creative Commons

Xuyan Zhao,

Yuan Zhang, Qunfeng Niu

et al.

Sensors, Journal Year: 2024, Volume and Issue: 24(6), P. 1989 - 1989

Published: March 20, 2024

Lateral flow immunoassay (LFIA) is extensively utilized for point-of-care testing due to its ease of operation, cost-effectiveness, and swift results. This study investigates the dynamics reaction mechanisms in LFIA by developing a three-dimensional model using Richards equation porous media transport, employing numerical simulations through finite element method. The delves into transport diffusion behaviors each component both sandwich competitive under non-uniform conditions. Additionally, impact various parameters (such as reporter particle concentration, initial capture probe concentrations T-line C-line, rate constants) on performance analyzed. findings reveal that, LFIA, optimizing like increasing concentration T-line, well adjusting constants, can effectively enhance detection sensitivity broaden working range. Conversely, effects are inverse. offers valuable insights design enhancement assays.

Language: Английский

Fortified Dual-Spectral Overlap with Enhanced Colorimetric/Fluorescence Dual-Response Immunochromatography for On-Site Bimodal-Type Gentamicin Monitoring DOI
Sijie Liu, Cong Zhao,

Rui Shu

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: April 16, 2024

Immunochromatography (ICA) remains untapped toward enhanced sensitivity and applicability for fulfilling the nuts bolts of on-site food safety surveillance. Herein, we report a fortified dual-spectral overlap with colorimetric/fluorescence dual-response ICA bimodal-type gentamicin (Gen) monitoring by employing polydopamine (PDA)-coated AuNPs (APDA) simultaneously serving as colorimetric reporter fluorescence quencher. Availing response that originated from PDA layer, resultant APDA exhibits less required antibody immunoprobes in single immunoassay, which facilitates improved utilization efficiency immuno-recognition APDA-ICA. Further integrated advantageous features excitation emission Arg/ATT-AuNCs, this APDA-ICA "turn on/off" pattern achieves visual limits detection 1.0 0.5 ng mL–1 patterns (25- 50-fold lower than standard AuNPs-ICA). Moreover, excellent self-calibration satisfactory recovery 79.03–118.04% were shown colorimetric–fluorescence analysis Gen real environmental media (including river water, an urban aquaculture water body, aquatic product, animal byproduct). This work provides feasibility exploiting dual safeguarding public health.

Language: Английский

Citations

10
Enhancing the stability and sensitivity of a lateral flow immunoassay using stabilized AuNPs for the simultaneous detection of sulfonamides and antibacterial synergists in chicken meat DOI
Chen Chen,

Baowei Huang,

Wenqing Xu

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 412, P. 135812 - 135812

Published: April 23, 2024

Language: Английский

Citations

10
Ferrous Tungstate Nanomaterials with Excellent Enzyme-Mimicking Activity to Enhance Lateral Flow Immunoassay Sensitivity DOI

Xianqing Tang,

Hua Zhang, Jinghuang Chen

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: 97(5), P. 2714 - 2723

Published: Jan. 30, 2025

Lateral flow immunochromatography (LFIA) with gold nanoparticles (AuNPs) is widely used in the biomedical field as a rapid and simple vitro detection technique. However, conventional AuNP-LFIA has limitations sensitivity range. In this study, nonprecious metal iron-based bimetallic FeWO4 nanomaterials convenient excellent enzyme-mimetic catalytic activities were synthesized by one-pot hydrothermal method. Here, combined C-reactive protein (CRP) antibodies to form novel signal-enhancing probe for analysis of CRP. The further achieves significant signal amplification catalyzing oxidation reaction 3-amino-9-ethylcarbazole LFIA assay, thereby improving accuracy assay. application FeWO4-based improved limit CRP 19.38 ng/mL after amplification, which approximately 30-fold lower than that addition, method demonstrated good stability reproducibility, providing promising prospective strategy early diagnosis inflammatory diseases.

Language: Английский

Citations

1
A handheld fluorescent lateral flow immunoassay platform for highly sensitive point-of-care detection of methamphetamine and tramadol DOI
Weijia Li, Xingsheng Yang, Dongfeng Wang

et al.

Talanta, Journal Year: 2024, Volume and Issue: 277, P. 126438 - 126438

Published: June 18, 2024

Language: Английский

Citations

8
PEI-Mediated Assembly of Fe3O4 onto SiO2-Encapsulated CsPbBr3 for Highly Sensitive Fluorescent Lateral Flow Immunoassay DOI
Yanxue Shang, Jinling Wang,

Hongkun Xia

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(15), P. 6065 - 6071

Published: April 3, 2024

The conventional lateral flow immunoassay (LFIA) method using colloidal gold nanoparticles (Au NPs) as labeling agents faces two inherent limitations, including restricted sensitivity and poor quantitative capability, which impede early viral infection detection. Herein, we designed synthesized CsPbBr3 perovskite quantum dot-based composite nanoparticles, CsPbBr3@SiO2@Fe3O4 (CSF), integrated fluorescence detection magnetic enrichment properties into LFIA technology achieved rapid, sensitive, convenient of the SARS-CoV-2 virus N protein. In this study, served a high-quantum-yield fluorescent signaling probe, while SiO2 significantly enhanced stability biomodifiability CsPbBr3. Importantly, shell shows relatively low absorption or scattering toward fluorescence, maintaining yield up to 74.4% in CsPbBr3@SiO2. Assembly Fe3O4 mediated by PEI further method's reduced matrix interference through enrichment. Consequently, range 1 × 102 5 106 pg·mL–1 after enrichment, with limit (LOD) 58.8 pg·mL–1, representing 13.3-fold improvement compared nonenriched samples (7.58 pg·mL–1) 2-orders-of-magnitude over commercial kits. Furthermore, exhibited 80% positive 100% negative rates clinical samples. This approach holds promise for on-site diagnosis, home-based tests, disease procession evaluation.

Language: Английский

Citations

6
Tailored Ultrastable Core–Shell Au@Ag Nanoparticles for Enhanced Colorimetric Detection in Lateral Flow Assays DOI
Bryan Gosselin, Gilles Bruylants, Ivan Jabin

et al.

ACS Applied Nano Materials, Journal Year: 2024, Volume and Issue: 7(6), P. 6169 - 6177

Published: March 5, 2024

In the quest for more effective colorimetric reporters compared with traditional gold nanoparticles (AuNPs), a family of Au@Ag core–shell was designed and synthesized using seed growth-mediated approach starting from commercial 37 nm AuNPs. This method enabled precise control over thickness silver shell by employing hydroquinone reduction citrate stabilization resulting particles. Core–shell NPs an Ag 7 (Au@Ag5NPs) 18 (Au@Ag10NPs) were synthesized, in orange milky yellow suspension, respectively. Additionally, impact external layer on Au@Ag10NPs (Au@Ag10@AuNPs), which significantly altered their optical properties to gray, investigated. The Au@AgNPs exhibited substantially higher molar extinction coefficients than parent AuNPs: 3.5-fold Au@Ag5NPs 9-fold Au@Ag10@AuNPs. Subsequently, all functionalized calix[4]arene layer, imparting superior stability against stresses, such as dispersion PBS, when ligands. calixarene coating covalent bioconjugation antibodies NP types without inducing noticeable aggregation. Their performance evaluated lateral flow assay troponin I detection, demonstrating positive signals down 1 ng/mL, surpassing detection limit (2.5 ng/mL). Notably, gray color Au@Ag10@AuNPs provided strong contrast white NC membrane, facilitating T-line visualization even at low signal intensity. Despite lack optimization our LFA, it competes quantification LFAs offering potential development highly sensitive assay. diverse employed this study form library distinct properties, paving way multiplexed systems targeting multiple proteins simultaneously enhancing diagnostic reliability. Furthermore, choice allows tailoring range based pertinent desired analyte, dictated reporter's light properties.

Language: Английский

Citations

5
Highly Efficient Fabrication of Fluorescent “Turn-On” Lateral Flow Strips for Highly Sensitive Detection of Small Molecules Based on Self-Assembly of AuAg Nanoclusters DOI

Mengjia Chao,

Qiuli Pan,

Guowen Li

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(19), P. 7714 - 7722

Published: April 30, 2024

Currently, fluorescent "turn-on" lateral flow assay (FONLFA) has shown enhanced "naked eye" detection sensitivity for small molecules, while it is urgent to adopt biocompatible nanomaterials and needs new strategies simplify the preparation process. In this study, a highly effective method was proposed produce FONLFA strips of molecules. The gold–silver nanoclusters (AuAgNCs) were immobilized onto nitrocellulose membrane by self-assembly poly(sodium 4-styrenesulfonate), antigen, AuAgNCs. immobilization process entails straightforward mixing three components, taking merely 1 min, thereby bypassing necessity chemical modification nanomaterials. strategy offers significantly simplified process, which substantially enhances efficiency strip fabrication. Utilizing method, developed carbendazim with visual limit (vLOD) reduced 40-fold compared conventional colorimetric (LFA). Furthermore, approach demonstrates versatility enabling AuAgNCs streptavidin, facilitates development aptamer-based FONLFAs. designed kanamycin exhibited 50-fold reduction in vLOD LFAs. Therefore, holds promising potential widespread applications analysis

Language: Английский

Citations

5
Plasmonic-Enhanced Colorimetric Lateral Flow Immunoassays Using Bimetallic Silver-Coated Gold Nanostars DOI
Supriya Atta, Yuanhao Zhao,

Sebastian Sanchez

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 29, 2024

The colorimetric lateral flow immunoassay (cLFIA) has gained widespread attention as a point-of-care testing (POCT) technique due to its low cost, short analysis time, portability, and capability of being performed by unskilled operators with minimal requirement reagents. However, the analytical sensitivity conventional LFIA based on colloidal gold nanospheres limits their applications for sensitive detection trace amounts target analytes. In this study, we introduced novel plasmonic-enhanced (PE-cLFIA) platform featuring bimetallic silver-coated nanostars (BGNS) exceptional optical properties, leading ultrahigh visual color brightness. BGNS-based PE-cLFIA was successfully applied detect model analyte, low-calcium response V (LcrV), virulence protein factor found in

Language: Английский

Citations

5
Recent advances in lateral flow immunoassay based on sandwich format for whole-cell pathogen detection DOI
Min Yang, Xinxin Xu, Meng Zhang

et al.

Coordination Chemistry Reviews, Journal Year: 2025, Volume and Issue: 533, P. 216538 - 216538

Published: Feb. 21, 2025

Language: Английский

Citations

0
Advances in Paper-based Colorimetric Sensors Based on Nanozymes for Point-of-Care Testing DOI

Shuang Zhou,

Xiang Mei, Shaowei Wang

et al.

Acta Chimica Sinica, Journal Year: 2025, Volume and Issue: 83(2), P. 170 - 170

Published: Jan. 1, 2025

Language: Английский

Citations

0