Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
97(1), P. 584 - 593
Published: Dec. 31, 2024
Mitochondrial
DNA
(mtDNA)
damage
is
a
prevalent
phenomenon
that
has
been
proven
to
be
implicated
in
wide
spectrum
of
diseases.
However,
the
progressive
attenuation
probe
signals
response
mtDNA
within
living
cells
inherently
limits
sensitivity
and
precision
current
probes
for
detecting
damage.
Herein,
we
employ
an
innovative
organelle
signal
ratio
imaging
approach,
utilizing
mitochondria–nucleus
migration
MCQ,
achieve
unparalleled
cells.
MCQ
exhibited
initial
preferential
binding
mtDNA,
facilitated
by
its
cationic
quinolinium
moiety,
but
migrated
nucleus
upon
This
unique
behavior
not
only
enhanced
spatial
identifiability
also
amplified
detection
significantly
harnessing
intensified
against
attenuated
mitochondrial
signal.
approach
established
positive
correlation
between
damage,
enabling
even
subtle
at
early
stage
apoptosis
with
remarkable
23-fold
enhancement
following
just
5
min
H2O2
induction
cells,
whereas
conventional
methods
relying
solely
on
fading
proved
insufficient.
Furthermore,
MCQ's
ability
monitor
occurrence
achieved
intricate
differentiation
ferroptosis.
By
monitoring
drug-induced
cancer
was
further
conducted
using
evaluate
therapeutic
efficacy
four
anticancer
drugs
very
low
concentrations.
strategy
paves
way
ultrasensitive
holds
immense
promise
damage-associated
Bulletin of the Korean Chemical Society,
Journal Year:
2024,
Volume and Issue:
45(8), P. 699 - 705
Published: Aug. 1, 2024
Abstract
Hydrogen
sulfide
(H
2
S)
and
nitroreductases
(NTR)
are
key
biological
reductants,
with
H
S
acting
as
a
signaling
molecule
NTR
reducing
nitro
groups
to
amines
in
cellular
metabolism.
Visualizing
these
components,
often
overexpressed
cancer
cells
or
bacterial
environments,
is
crucial
for
metabolic
understanding.
Existing
fluorescent
probes
detect
individually,
but
simultaneous
detection
methods
unexplored.
We
designed
probe
1
,
which
shows
high
fluorescence
only
when
both
overexpressed.
The
transformed
into
benzothiazole‐fused
coumarin
by
NTR,
exhibiting
fluorescence,
stronger
signal
the
presence
of
S,
making
it
potential
tool
detecting
their
overexpression.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Aug. 30, 2024
Mitochondrial
polarity
is
a
critical
indicator
of
numerous
pathological
and
biological
processes;
thus,
the
development
fluorescent
probes
capable
targeting
mitochondria
visually
monitoring
its
great
significance.
In
this
study,
were
designed
with
N,
N-dialkylamino
rhodol
scaffold
as
fluorophore
sensitive
to
environments,
in
which
alkyl
chain
length
was
adjusted
rationally
obtain
distinct
recognition
modes.
By
integrating
groups,
three
fluorogenic
chemical
