Bifunctional MOF-Encapsulated Cobalt-Doped Carbon Dots Nanozyme-Powered Chemiluminescence/Fluorescence Dual-Mode Detection of Aflatoxin B1

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(13), P. 16494 - 16504

Published: March 20, 2024

A novel bifunctional MOF-encapsulated cobalt-doped carbon dots nanozyme (Co-CD/PMOF) with excellent peroxidase-mimic catalytic activity and fluorescence property was synthesized employed to fabricate a chemiluminescence/fluorescence (CL/FL) dual-mode immunosensor for AFB1 detection. Co-CD/PMOF could catalyze the luminol/H2O2 system generate robust long-lasting CL signals due slow diffusion effect continuous generation of •OH, O2•–, 1O2 species. Differing from traditional flash-type emissions, this glow-type emission is helpful sensitive accurate sensing platform. Then CL/FL detection developed using antibody-functionalized as signal-amplifying nanoprobe. The mode assay based on indirect competitive immune principle carried out chemiluminescence optical fiber platform, where AFB1-OVA-functionalized probe biorecognition, separation, signal conducting. range LOD were 0.63–69.36 ng/mL 0.217 ng/mL, respectively. Using immunomagnetic beads capturing FL established sandwich principle. linear 0.54–51.91 0.027 obtained. This work designed sensitive, rapid, reliable nanozyme-powered strategy provided technical support in field environmental monitoring food safety.

Language: Английский

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Electroless deposition of platinum on graphdiyne for electrochemical sensing

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 497, P. 154512 - 154512

Published: Aug. 3, 2024

Language: Английский

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Renewable Electrochemiluminescence Biosensor Based on Eu-MOGs as a Highly Efficient Emitter and a DNAzyme-Mediated Dual-drive DNA Walker as a Signal Amplifier for Ultrasensitive Detection of miRNA-222

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(11), P. 4589 - 4596

Published: March 5, 2024

Herein, novel europium metal–organic gels (Eu-MOGs) with excellent cathode electrochemiluminescence (ECL) emission are first used to construct biosensors for the ultrasensitive detection of miRNA-222. Impressively, N and O elements organic ligand 2,2′:6,2″-terpyridine 4,4′,4″-tricarboxylic acid (H3-tctpy) can perfectly coordinate Eu3+ form Eu-MOGs, which not only reduce nonradiative transition caused by intramolecular free rotation phenyl rings in other MOGs enhance ECL signal extraordinary efficiency as high 37.2% (vs [Ru(bpy)3]2+/S2O82– system) but also reinforce ligand-to-metal charge transfer (LMCT) strong affinity between greatly improve stability signals. Besides, an improved nucleic cascade amplification reaction is developed raise conversion from target miRNA-222 a DNAzyme-mediated dual-drive DNA walker output DNA, simultaneously shear specific recognition sites two directions. In that way, proposed biosensor further sensitivity linear range 10 aM–1 nM limit (LOD) 8.5 aM, achieve accurate response cancer cell lysates MHCC-97L HeLa. Additionally, be self-regenerated folding/unfolding related triplets pH changes simplify experimental operations cost. Hence, this work stable intense signals construction renewable biosensor, supplying reliable method biomarker analysis disease diagnosis.

Language: Английский

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Fe–Co-Based Metal–Organic Frameworks as Peroxidase Mimics for Sensitive Colorimetric Detection and Efficient Degradation of Aflatoxin B₁

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(9), P. 11809 - 11820

Published: Feb. 22, 2024

Building multifunctional platforms for integrating the detection and control of hazards has great significance in food safety environment protection. Herein, bimetallic Fe–Co-based metal–organic frameworks (Fe–Co-MOFs) peroxidase mimics are prepared applied to develop a bifunctional platform synergetic sensitive controllable degradation aflatoxin B1 (AFB1). On one hand, Fe–Co-MOFs with excellent peroxidase-like activity combined target-induced catalyzed hairpin assembly (CHA) construct colorimetric aptasensor AFB1. Specifically, binding aptamer AFB1 releases prelocked Trigger initiate CHA cycle between H2-modified H1-tethered magnetic nanoparticles form complexes. After separation, signal supernatant presence TMB H2O2 is inversely proportional target contents. Under optimal conditions, this biosensor enables analysis limit 6.44 pg/mL, high selectivity satisfactory recovery real samples obtained. other remarkable Fenton-like catalytic performance organic contaminants further used detoxification after detection. The almost completely removed within 120 min. Overall, introduction improves sensing sensitivity; efficient postcolorimetric-detection reduces secondary contamination risk experimental operators. This strategy expected provide ideas designing integrate various hazards.

Language: Английский

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Nanocellulose and multi-walled carbon nanotubes reinforced polyacrylamide/sodium alginate conductive hydrogel as flexible sensor

Journal of Colloid and Interface Science, Journal Year: 2024, Volume and Issue: 677, P. 692 - 703

Published: Aug. 13, 2024

Language: Английский

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Enhancing 3D DNA Walker-Induced CRISPR/Cas12a Technology for Highly Sensitive Detection of ExomicroRNA Associated with Osteoporosis

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(3), P. 1438 - 1446

Published: March 7, 2024

Exosomal microRNAs (exomiRNAs) have emerged as promising biomarkers for the early clinical diagnosis of osteoporosis. However, their limited abundance and short length in peripheral blood present significant challenges accurate detection exomiRNAs. Herein, we designed implemented an efficacious fluorescence-based biosensor highly sensitive exomiRNA associated with osteoporosis, leveraging enhancing 3D DNA walker-induced CRISPR/Cas12a technology. The engineered walker is capable efficiently transforming target into amplifying strands, thereby sensitivity developed biosensor. Concurrently, liberated strands serve activators to trigger Cas12a trans-cleavage activity, culminating a significantly amplified fluorescent signal exomiRNA-214. Under optimal conditions, devised technology demonstrated capacity detect exomiRNA-214 at concentrations low 20.42 fM, encompassing wide linear range extending from 50.0 fM 10.0 nM. Moreover, could accurately differentiate between healthy individuals osteoporosis patients via exomiRNA-214, which was agreement RT-qPCR results. As such, this biosensing offers promise valuable tool

Language: Английский

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Dual-responsive 3D DNA nanomachines cascaded hybridization chain reactions for novel self-powered flexible microRNA-detecting platform

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 252, P. 116149 - 116149

Published: Feb. 20, 2024

Language: Английский

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A hierarchical nano-MoS₂ flake/micro-MXene lamellar complex structure within a carbon coating for rapid sodium-ion storage

Journal of Materials Chemistry A, Journal Year: 2024, Volume and Issue: 12(11), P. 6329 - 6340

Published: Jan. 1, 2024

A hierarchical nano-MoS 2 /micron-MXene composite is designed by a pre-intercalation–sulphuration concept and polypyrrole-derived carbon “protective framework”. The MSVC@C anode exhibits high capacity prominent rate performance for SIBs.

Language: Английский

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Novel Self-Powered Biosensor Based on Au Nanoparticles @ Pd Nanorings and Catalytic Hairpin Assembly for Ultrasensitive miRNA-21 Assay

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(36), P. 14508 - 14515

Published: Aug. 23, 2024

An ultrasensitive self-powered biosensor is constructed for miRNA-21 detection based on Au nanoparticles @ Pd nanorings (Au NPs@Pd NRs) and catalytic hairpin assembly (CHA). The NRs possess excellent electrical conductivity to improve the electron transfer rate show good elimination of byproduct H2O2 assist glucose oxidase (GOD) catalyze glucose; CHA used as an amplification strategy effectively enhance sensitivity biosensor. To further amplify output signal, a capacitor integrated into With multiple signal strategies, possesses linear range 0.1–10–4 fM lower limit (LOD) 0.032 (S/N = 3). In addition, as-prepared displays potential applicability in assay toward human serum samples.

Language: Английский

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A self-powered biosensor with cascade amplification capability facilitates ultra-sensitive detection of microRNA biomarkers

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 275, P. 117233 - 117233

Published: Feb. 8, 2025

Language: Английский

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