All-In-One Entropy-Driven DNA Nanomachine for Tumor Cell-Selective Fluorescence/SERS Dual-Mode Imaging of MicroRNA

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

An entropy-driven catalysis (EDC) strategy is appealing for amplified bioimaging of microRNAs in living cells; yet, complex operation procedures, lacking cell selectivity, and insufficient accuracy hamper its further applications. Here, we introduce an ingenious all-in-one DNA nanomachine (termed as AIO-EDN), which can be triggered by endogenous apurinic/apyrimidinic endonuclease 1 (APE1) to achieve tumor cell-selective dual-mode imaging microRNA. Compared with the traditional EDC strategy, integrated design AIO-EDN achieves autocatalytic signal amplification without extra fuel strands. Moreover, leverages APE1 overexpressed cancer cells activate reaction, which, however, exerts no target sensing activity normal cells. Combining fluorescence- surface-enhanced Raman scattering (FL/SERS) techniques, this exhibits significantly improved selectivity microRNA This study provides a new paradigm develop EDC-based platform shows great potential in-depth diagnosis high precision.

Language: Английский

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A self-propelled DNAzyme-mediated cascading entropy-driven catalysis DNA nanodevice for high-efficiency imaging in living cells and mouse bodies under near-infrared light initiation

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 496, P. 154268 - 154268

Published: July 24, 2024

Language: Английский

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Near-infrared light-responsive DNA nanosensor with hyperbranched self-stacking exponential amplification for sensitive and precise live-cell and in vivo imaging

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: 428, P. 137259 - 137259

Published: Jan. 16, 2025

Language: Английский

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Construction of a smart dual-responsive targeted drug nanocarrier for imaging and treatment of breast cancer cells

Bioorganic Chemistry, Journal Year: 2025, Volume and Issue: 157, P. 108284 - 108284

Published: Feb. 15, 2025

Language: Английский

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A dual-switch fluorescence biosensor with entropy-driven and DNA walker cascade amplification circuit for sensitive microRNA detection

Nanoscale, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

To achieve precise diagnosis of tumor cells, designing nucleic acid amplification circuits with intelligent multi-switch responsiveness for the specific and sensitive detection miRNA within cells is an important strategy. Here, we developed a dual-switch fluorescence biosensor that integrates two-step cascade signal circuit entropy-driven (EDC) DNA walkers onto gold nanoparticles highly quantitative target in cells. The dual switches trigger are APE1 enzyme, both which upregulated be specific, miRNA21 triggers upstream EDC, releasing strands serve as downstream circuit. Under cleavage-driven action walker walk along track on surface AuNPs, strong presenting good linearity concentration range 40 pM-100 nM. This presents specificity, anti-interference ability against multiple RNAs enzymes can effectively distinguish cancer from normal cell lysates. Overall, this provides recognition efficient strategy tumors, indicating its potential clinical applications disease diagnosis.

Language: Английский

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Dual-palindrome-incorporated hand-in-hand self-linking bidirectional DNA amplifier within exogenous near-infrared light stimulation for high-performance imaging in living biosystems

Talanta, Journal Year: 2025, Volume and Issue: 292, P. 128003 - 128003

Published: March 26, 2025

Language: Английский

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An Enzyme-Free and Label-Free Multiplex Detection of miRNAs by Entropy-Driven Circuit Coupled with Capillary Electrophoresis

Talanta, Journal Year: 2024, Volume and Issue: 281, P. 126850 - 126850

Published: Sept. 12, 2024

Language: Английский

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Early Diagnosis of Triple-Negative Breast Cancer Based on Dual microRNA Detection Using a Well-Defined DNA Crown-Carbon Dots Structure as an Electrochemiluminescence Sensing Platform

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(45), P. 17984 - 17992

Published: Oct. 31, 2024

Triple-negative breast cancer (TNBC) is the most aggressive subtype of (BC). Thus, early detection and accurate diagnosis this are crucial for improving survival rate patients. Specific microRNAs (miRNAs) have been implicated in occurrence, proliferation, metastasis TNBC. Addressing need, our study developed a biosensor platform TNBC by integrating electrochemiluminescence (ECL) technology with DNA sensing strategy. Specifically, synthesized positively charged carbon dots (CDs) were used to neutralize electrostatic repulsion between strands facilitate assembly triangular prisms (DNA TP-CDs). Hairpins then incorporated into TP-CDs form final crown structure. The biomarker, microRNA-93–3p (miR-93–3p), allowed binding Crown track on electrode initiated ECL signal. Subsequently, microRNA-210 (miR-210) unlocked tripedal walker, its movement eventually quenched signal, enabling tumor stage assessment. Our proposed had satisfactory efficiency due ordered rapid-moving walker. data revealed good linear relationship signals logarithm miRNA concentrations, miR-93–3p having limit 31.04 aM miR-210 7.69 aM. also showed performance serum samples cells. Taken together, hopes provide ideas applications clinical as well personalized treatment

Language: Английский

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Entropy driven-based catalytic biosensors for bioanalysis: From construction to application-A review

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1338, P. 343549 - 343549

Published: Dec. 20, 2024

Language: Английский

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