Design and Characterization of a Gene-Encoding DNA Nanoparticle in a Cell-Free Transcription–Translation System

ACS Applied Nano Materials, Journal Year: 2024, Volume and Issue: 7(11), P. 12891 - 12902

Published: May 30, 2024

DNA nanotechnology has made initial progress toward developing gene-encoded origami nanoparticles (NPs) that display potential utility for future gene therapy applications. However, due to the challenges involved with delivery into cells including transport through membrane, intracellular targeting, and inherent expression of nucleases along interference from other active proteins, it can be difficult more directly study effect NP design on subsequent expression. In this work, we demonstrate an approach studying gene-encoding NPs without use cells. We utilize a pure E. coli-derived cell-free transcription-translation (TXTL) system, which is composed optimized components bacterial expression, benchtop studies assess how promoter sequence in conjunction structural template affects The Renilla luciferase was first amplified single-stranded (ss) scaffold strand then folded 12-helix bundle exogenous staple strands as test platform. Using luciferase-based bioluminescence assays characterize relative protein level, found still transcribed when folded, albeit at lower rate than double-stranded segment. On comparing three variants different configurations, results indicate designed remain ssDNA form reduced NP, replacing arbitrary significantly lowers This work demonstrates power TXTL systems aid capabilities development

Language: Английский

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Tuning assembled DNA nanoarchitecture into disassembled state to enhance accurate and ultrasensitive detection of KRAS G12D mutation

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 420, P. 136513 - 136513

Published: Aug. 23, 2024

Language: Английский

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Radiation and DNA Origami Nanotechnology: Probing Structural Integrity at the Nanoscale

ChemPhysChem, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 30, 2024

Abstract DNA nanotechnology has emerged as a groundbreaking field, using scaffold to create nanostructures with customizable properties. These hold potential across various domains, from biomedicine studying ionizing radiation‐matter interactions at the nanoscale. This review explores how types of radiation, covering spectrum electrons and photons sub‐excitation energies ion beams high‐linear energy transfer influence structural integrity origami nanostructures. We discuss both direct effects those mediated by secondary species like low‐energy (LEEs) reactive oxygen (ROS). Further we possibilities for applying radiation in modulating controlling changes. Based on experimental insights, identify current challenges characterizing responses outline further areas investigation. not only clarifies complex dynamics between but also suggests new strategies designing optimized applications exposed qualities their resulting byproducts.

Language: Английский

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Plugging synthetic DNA nanoparticles into the central dogma of life

Chemical Communications, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Recent advancements have engineered an approach to encode genetic sequences in DNA nanoparticles, making them a promising platform for gene therapy. This article highlights design considerations of these structures optimal protein expression.

Language: Английский

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