Talanta, Journal Year: 2024, Volume and Issue: 286, P. 127457 - 127457
Published: Dec. 24, 2024
Language: Английский
ACS Synthetic Biology, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 29, 2025
Advancements in molecular diagnostics, such as polymerase chain reaction and next-generation sequencing, have revolutionized disease management prognosis. Despite these advancements the field faces challenges due to high operational costs need for sophisticated equipment highly trained personnel besides having several technical limitations. The emergent of CRISPR/Cas sensing technology is showing promise a new paradigm clinical although widespread adoption remains limited. This perspective paper discusses specific cases where can surmount existing diagnostic methods by stressing significant role that play revolutionizing diagnostics. It underscores urgency importance addressing technological regulatory hurdles must be overcome harness this effectively laboratories.
Language: Английский
Citations
1
Talanta, Journal Year: 2025, Volume and Issue: 291, P. 127815 - 127815
Published: Feb. 27, 2025
Language: Английский
Citations
1
Critical Reviews in Food Science and Nutrition, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 16
Published: April 24, 2024
Global food safety stands out as a prominent public concern, affecting populations worldwide. The recurrent challenge of incidents reveals the need for robust inspection framework. In recent years, integration isothermal nucleic acid amplification with CRISPR-Cas12a techniques has emerged promising tool molecular detection hazards, presenting next generation biosensing detection. This paper provides comprehensive review current state research on synergistic application and technology in field safety. innovative combination not only enriches analytical tools, but also improving assay performance such sensitivity specificity, addressing limitations traditional methods. summarized various methodologies by diverse concerns, including pathogenic bacterium, viruses, mycotoxins, adulteration, genetically modified foods. Each section elucidates specific strategies employed highlights advantages conferred. Furthermore, discussed challenges faced this context safety, offering insightful discussions potential solutions future prospects.
Language: Английский
Citations
6
Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 51(1)
Published: Aug. 8, 2024
Language: Английский
Citations
4
Micromachines, Journal Year: 2024, Volume and Issue: 15(11), P. 1329 - 1329
Published: Oct. 30, 2024
Foodborne pathogens pose significant risks to food safety. Conventional biochemical detection techniques are facing a series of challenges. In recent years, with the gradual development CRISPR (clustered regularly interspaced short palindromic repeats) technology, CRISPR/Cas system-based biosensors, newly emerging have received much attention from researchers because their supreme flexibility, sensitivity, and specificity. While numerous CRISPR-based biosensors broad application in field environmental monitoring, safety, point-of-care diagnosis, they remain high demand summarize advances for foodborne pathogen detection. this paper, we briefly classify discuss working principles systems trans-cleavage activity applications pathogenic microorganisms. We highlight current status, unique feature each system biosensing platforms, integration CRISPR-Cas other techniques, concluding discussion advantages, disadvantages, future directions.
Language: Английский
Citations
4
Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113055 - 113055
Published: Feb. 1, 2025
Language: Английский
Citations
0
Drug Discovery Today, Journal Year: 2025, Volume and Issue: unknown, P. 104362 - 104362
Published: April 1, 2025
Artificial intelligence (AI) and machine learning (ML) have revolutionized pharmaceutical research, particularly in protein nucleic acid studies. This review summarizes the current status of AI ML applications sector, focusing on innovative tools, web servers, databases. paper highlights how these technologies address key challenges drug development including high costs, lengthy timelines, complexity biological systems. Furthermore, potential personalized medicine, cancer response prediction, biomarker identification is discussed. The integration research promises to accelerate discovery, reduce ultimately lead more effective therapeutic strategies.
Language: Английский
Citations
0
Methods, Journal Year: 2025, Volume and Issue: unknown
Published: April 1, 2025
Language: Английский
Citations
0
Journal of Microbiological Methods, Journal Year: 2025, Volume and Issue: 235, P. 107148 - 107148
Published: May 13, 2025
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(12), P. 6336 - 6336
Published: June 7, 2024
Cell monitoring is essential for understanding the physiological conditions and cell abnormalities induced by various stimuli, such as stress factors, microbial invasion, diseases. Currently, techniques detecting metabolites originating from specific cells are employed to obtain information on in terms of human health. Although states have traditionally been accessed using instrument-based analysis, this has replaced sensor systems equipped with new materials technologies. Various developed recognizing biological markers proteins surfaces, components plasma membranes, secreted metabolites, DNA sequences. Sensor classified into subclasses, chemical sensors biosensors, based used recognize targets. In review, we aim outline fundamental principles cells, encompassing both biosensors sensors. Specifically, focus biosensing types sensing signal-transducing elements introduce recent advancements applications biosensors. Finally, address present challenges biosensor prospects that should be considered enhance performance. review covers application believe it can provide valuable insights researchers general readers interested its further biomedical fields.
Language: Английский
Citations
2