Cloud-enabled Scalable Analysis of Large Proteomics Cohorts

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 10, 2024

Abstract Rapid advances in depth and throughput of untargeted mass-spectrometry-based proteomic technologies are enabling large-scale cohort proteogenomic analyses. As such studies scale, the data infrastructure search engines required to process must also scale. This challenge is amplified that rely on library-free match between runs (MBR) search, which enable enhanced depth-per-sample completeness. However, to-date, no MBR-based could scale cohorts thousands or more individuals. Here, we present a strategy deploy distributed cloud environment without source code modification, thereby enhancing resource scalability throughput. Additionally, an algorithm, Scalable MBR, replicates MBR procedure popular DIA-NN software for samples. We demonstrate can MS raw files few hours compared days original results almost indistinguishable those native MBR. The method has been tested over 15,000 injections available use Proteograph™ Analysis Suite.

Language: Английский

Regional and longitudinal dynamics of human milk protein components assessed by proteome analysis on a fast and robust micro-flow LC–MS/MS system

Food Chemistry, Journal Year: 2024, Volume and Issue: 465, P. 141981 - 141981

Published: Nov. 9, 2024

Language: Английский

Autonomous Dissociation-type Selection for Glycoproteomics Using a Real-Time Library Search

Journal of Proteome Research, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 12, 2024

Tandem mass spectrometry (MS/MS) is the gold standard for intact glycopeptide identification, enabling peptide sequence elucidation and site-specific localization of glycan compositions. Beam-type collisional activation generally sufficient

Language: Английский