Single-Electrode Flow Cell for Electrochemiluminescent Flow Analysis DOI
Zhiyong Dong,

Yequan Chen,

Shiyu Xia

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 20, 2024

Flow injection analysis and liquid chromatography are frequently combined with electrochemiluminescence (ECL) for flow analysis. Almost all electrochemistry analyses employ traditional three-electrode electrochemical cells which have working electrode, counter reference electrode; however, it is expensive difficult to fabricate a cell inconvenient renew the electrode. In this study, we developed single-electrode using commercially available conductive polyethylene film as only electrode through potential differences induced by resistance first time. The features simple structure, easy renewal of low cost compared cells. Taking typical Ru(bpy)

Language: Английский

Programmable DNA Templates for Silver Nanoclusters Synthesis To Develop On-Demand FRET Aptasensor DOI
Yue Liu, Mustafa Hussain, Chengquan Wang

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(25), P. 10391 - 10398

Published: June 6, 2024

DNA-templated silver nanoclusters (AgNCs-DNA) can be synthesized via a one-pot method bypassing the tedious process of biomolecular labeling. Appending an aptamer to DNA templates results in dual-functionalized strands that utilized for synthesizing aptamer-modified AgNCs, thereby enabling development label-free fluorescence aptasensors. However, major challenge lies necessity redesign strand each specific target, thus increasing complexity and hindering widespread application these To overcome this challenge, we designed six (DNA1-DNA6) incorporate AgNCs synthesis A4-linker further coupling. Among all AgNCs-DNA samples, it was found both AgNCs-DNA1 AgNCs-DNA2 stood out their excellent long-term stability. After capturing T4-linker connected with aptamer1 aflatoxin B1 (AFB1), however, only AgNCs-DNA1/aptamer1 maintained This finding highlighted potential as versatile probe on-demand emphasize its benefits aptasensing applications, MoS

Language: Английский

Citations

9
A visual biosensor chip based on the enhanced electrochemiluminescence with low triggered potential for ochratoxin A detection DOI
Liqi Liu, Yu Qiu, Yi Zou

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 420, P. 136461 - 136461

Published: Aug. 13, 2024

Language: Английский

Citations

5
Lectin-Mediated Labeling of Alkaline Phosphatase for Enzymatic Silver Deposition-Based Electrochemical Detection of Glycoprotein Tumor Markers DOI

Junpeng Lv,

Songmin Chen, Wenhui Xu

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

The screening of glycoprotein markers has become an integral part the in vitro diagnosis malignant tumors. Herein, electrochemical method based on alkaline phosphatase (ALP)-mediated enzymatic silver deposition is reported for highly sensitive detection tumor markers, which ALP enzymes are decorated to glycan moieties targets via lectin-carbohydrate interactions. As glycoproteins conjugated with multiple chains, lectin-mediated labeling can result decoration each target enzymes. Moreover, hydrolysis ascorbic acid 2-phosphate into a high density particles, then be sensitively assayed robust Ag/AgCl solid-state voltammetric process. result, deposition-based exhibits sensitivity. Using aptamer-based breast cancer-associated CA15-3 as proof concept, limit 0.32 mU/mL been demonstrated. Results show that synergism capture and glycoform-specific discrimination capability interactions endow this selectivity, its practical use assay levels serum samples illustrated. With benefits from efficiency, mild reaction conditions, user-friendly operation, applicable low-abundance markers.

Language: Английский

Citations

0
The development of electrochemiluminescent probes: Mechanism and application DOI

Tongtong Li,

Yingying Su, Lichun Zhang

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113216 - 113216

Published: March 1, 2025

Language: Английский

Citations

0
Advanced Electrochemiluminescence Biosensing Enabled by High-Performance Lipophilic [AuAg24(SPhMe2)18]– Nanoclusters Coupled with Bipolar Electrochemistry DOI

Hongfei Ji,

Mingyue Chen, Yuzhu Sun

et al.

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137840 - 137840

Published: April 1, 2025

Language: Английский

Citations

0
Single-Electrode Flow Cell for Electrochemiluminescent Flow Analysis DOI
Zhiyong Dong,

Yequan Chen,

Shiyu Xia

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 20, 2024

Flow injection analysis and liquid chromatography are frequently combined with electrochemiluminescence (ECL) for flow analysis. Almost all electrochemistry analyses employ traditional three-electrode electrochemical cells which have working electrode, counter reference electrode; however, it is expensive difficult to fabricate a cell inconvenient renew the electrode. In this study, we developed single-electrode using commercially available conductive polyethylene film as only electrode through potential differences induced by resistance first time. The features simple structure, easy renewal of low cost compared cells. Taking typical Ru(bpy)

Language: Английский

Citations

0