Rapid point-of-care pathogen sensing in the post-pandemic era DOI

Younju Joung,

Kihyun Kim, Jing An

et al.

Trends in biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Language: Английский

Simultaneous Detection of Five Infectious Diseases in a Single Strip: Oligo dT-Utilized Lateral Flow Immunoassay DOI
Jinwoo Kim,

Jungmin Nam,

Jeong‐Eun Park

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: 97(1), P. 775 - 782

Published: Jan. 2, 2025

The need for accurate and simultaneous diagnosis of multiple respiratory infectious diseases has become increasingly critical due to ongoing viral mutations the similarity symptoms among various viruses. Here, we have advanced our detection capabilities by developing a multiplex lateral flow immunoassay (LFA) platform that integrates oligonucleotides antibodies, enabling five viruses: Severe acute syndrome coronavirus 2 (SARS-CoV-2), Influenza A (FluA), B (FluB), Respiratory syncytial virus (RSV), Adenovirus (ADV), on single membrane. By applying oligonucleotide antibody-conjugated AuNPs, enables highly sensitive specific detection. In addition, signal amplification using RPA70A-conjugated gold nanoparticles developed in previous study can further be applied optionally low-concentration biomarkers. Our interferences cross-reactivity tests confirmed these complexes do not produce false positives, substantiating assay's utility clinical settings. This platform, therefore, provides robust solution precise rapid complex infections, positioning it as suitable application pandemic response scenarios.

Language: Английский

Citations

2
Programmable DNA Templates for Silver Nanoclusters Synthesis To Develop On-Demand FRET Aptasensor DOI
Yue Liu, Mustafa Hussain, Chengquan Wang

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(25), P. 10391 - 10398

Published: June 6, 2024

DNA-templated silver nanoclusters (AgNCs-DNA) can be synthesized via a one-pot method bypassing the tedious process of biomolecular labeling. Appending an aptamer to DNA templates results in dual-functionalized strands that utilized for synthesizing aptamer-modified AgNCs, thereby enabling development label-free fluorescence aptasensors. However, major challenge lies necessity redesign strand each specific target, thus increasing complexity and hindering widespread application these To overcome this challenge, we designed six (DNA1-DNA6) incorporate AgNCs synthesis A4-linker further coupling. Among all AgNCs-DNA samples, it was found both AgNCs-DNA1 AgNCs-DNA2 stood out their excellent long-term stability. After capturing T4-linker connected with aptamer1 aflatoxin B1 (AFB1), however, only AgNCs-DNA1/aptamer1 maintained This finding highlighted potential as versatile probe on-demand emphasize its benefits aptasensing applications, MoS

Language: Английский

Citations

9
Exploring the Diagnostic Landscape: Portable Aptasensors in Point-of-care Testing DOI
A. Mohan, Ipsita Roy

Analytical Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 115788 - 115788

Published: Jan. 1, 2025

Language: Английский

Citations

0
Nanobody-based strategy for rapid and accurate pathogen detection: A case of COVID-19 testing DOI

Wenjin Hu,

Yichen Liu, Xi Li

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 263, P. 116598 - 116598

Published: July 22, 2024

Language: Английский

Citations

2
Sensitive Colorimetric Lateral Flow Assays Enabled by Platinum‐Group Metal Nanoparticles with Peroxidase‐Like Activities DOI
Shikuan Shao, Xiaochuan Wang,

Caroline Sorial

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 6, 2024

The last several decades have witnessed the success and popularity of colorimetric lateral flow assay (CLFA) in point-of-care testing. Driven by increasing demand, great efforts been directed toward enhancing detection sensitivity CLFA. Recently, platinum-group metal nanoparticles (PGM NPs) with peroxidase-like activities emerged as a type promising labels for By incorporating simple rapid post-treatment process, PGM NP-based CLFAs are orders magnitude more sensitive than conventional gold nanoparticle-based CLFAs. In this perspective, study begins introducing design, synthesis, characterization NPs activities. current techniques surface modification then discussed, followed operation optimization Afterward, opinions provided on social impact Lastly, perspective is concluded an outlook future research directions emerging field, where challenges opportunities discussed.

Language: Английский

Citations

2
An Fe3O4-Au heterodimer nanoparticle-based lateral flow assay for rapid and simultaneous detection of multiple influenza virus nucleic acids DOI
Dong Yü,

Min Zi,

Yue Dou

et al.

Analytical Methods, Journal Year: 2024, Volume and Issue: 16(34), P. 5777 - 5784

Published: Jan. 1, 2024

An Fe 3 O 4 -Au nanoparticle-based LFA was developed for simultaneous detection of multiple influenza virus nucleic acids. This showed good accuracy and satisfactory sensitivity, could serve as a portable method early detection.

Language: Английский

Citations

1
Dually-amplified electrochemical aptasensor based on the self-linking AuPt nanoflowers for ultrasensitive determination of hemagglutinin DOI

Qingcai Yu,

Yan Wang, Yao Liu

et al.

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1329, P. 343257 - 343257

Published: Sept. 18, 2024

Language: Английский

Citations

0
Rapid point-of-care pathogen sensing in the post-pandemic era DOI

Younju Joung,

Kihyun Kim, Jing An

et al.

Trends in biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Language: Английский

Citations

0