Localized Bicirculating DNAzyme Self-Feedback Amplification Strategy for Ultra-Sensitive Fluorescence Biosensing of MicroRNA

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

DNAzyme-based cascade networks are effective tools to achieve ultrasensitive detection of low-abundance miRNAs. However, their designs complicated and costly, the operation is time-consuming. Herein, a novel simple noncascade DNAzyme network designed its amplification effect comparable or even better than many cascading ones. It nonenzymatic, isothermal, bicirculating consisting two toehold-mediated strand-displacement reactions localized strategy. Taking microRNA-122 as target model, this fluorescence biosensor has limit 84 zmol L–1, which 8-orders magnitude lower that nonamplification one. The ultrasensitivity mainly benefits from exclusive design positive self-feedback mechanism ingenious network. In addition, utilization superparamagnetic Fe3O4@SiO2 particles not only helps for localization DNAzymes but also facilitates rapid separation signal probes (output DNA-CdTe QDs). This fluorescent advantages specificity, speed, thermal stability, low cost. paves new way bioamplification strategy, may be very attractive biosensors, DNA logic gates, computers.

Language: Английский

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Isothermal nucleic acid amplification-based biosensors: The next generation analytical toolkit for point-of-care assay of foodborne pathogens

Trends in Food Science & Technology, Journal Year: 2025, Volume and Issue: unknown, P. 104882 - 104882

Published: Jan. 1, 2025

Language: Английский

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Distance-Based Visual miRNA Biosensor with Strand Displacement Amplification-Mediated DNA Hydrogel Assembly

ACS Materials Letters, Journal Year: 2024, Volume and Issue: 6(6), P. 2111 - 2117

Published: April 28, 2024

We have developed a novel paper biosensor for miRNA assays by monitoring the seepage flow distance along strip. Target miRNA-initiated strand displacement polymerization produces abundant DNA strands, which assist assembly of hydrogel. The phase transition is successfully demonstrated elastography and electrochemical techniques. variation highly related to viscosity By visual observation distance, sensitive quantification can be achieved. limit detection estimated 1 fM, quite low. utility in clinical samples also verified consistent results standard quantitative reverse transcription PCR (qRT-PCR) technique. This approach not only but exhibits merits convenient operation low cost, meeting requirements point-of-care testing. It shows significant potential applications biological studies disease diagnosis.

Language: Английский

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Sensitive fluorescence assay of chloramphenicol coupled with two-level isothermal amplification using a self-powered catalyzed hairpin assembly and entropy-driven circuit

Analytical Methods, Journal Year: 2024, Volume and Issue: 16(21), P. 3430 - 3437

Published: Jan. 1, 2024

A sensitive fluorescence assay of chloramphenicol was developed coupled with two-level isothermal amplification using a self-powered catalyzed hairpin assembly (CHA) and entropy-driven circuit (EDC).

Language: Английский

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Redox-responsive degradation of DNA@MnO2 nanoprobe triggering DNAzyme recycling amplification for sensitive and label-free detection of glutathione

Microchemical Journal, Journal Year: 2024, Volume and Issue: 204, P. 110983 - 110983

Published: June 13, 2024

Language: Английский

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Simultaneous detection of multiple microRNAs based on fluorescence resonance energy transfer under a single excitation wavelength

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2024, Volume and Issue: 322, P. 124788 - 124788

Published: July 5, 2024

Language: Английский

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