A Conformational Switch-Controlled RNA Sensor Based on Orthogonal dCas12a for RNA Imaging in Live Cells

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 273, P. 117185 - 117185

Published: Jan. 20, 2025

Language: Английский

Engineering a Novel NIR RNA-Specific Probe for Tracking Stress Granule Dynamics in Living Cells

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 21, 2025

Real-time monitoring of the dynamics cytosolic RNA-protein condensates, termed stress granules (SGs), is vital for understanding their biological roles in response and related disease treatment but challenging due to lack simple accurate methods. Compared with protein visualization that requires complex transfection procedures, direct RNA labeling offers an ideal alternative tracking SG living cells. Here, we propose a novel molecular design strategy construct near-infrared RNA-specific fluorescent probe (HQBT) SGs The positively charged HQBT was designed target negative groove RNA, its binding affinity significantly improved by adjusting position nitrogen atom molecule. Furthermore, additional hydroxyl group introduced achieve emission enhance RNA-binding capability. can rapidly stain within 5 s cells showed performance superior commercial SYTO RNA-Select dye terms photostability selectivity. Importantly, reversible assembly disassembly are successfully visualized using this RNA-selective imaging probe.

Language: Английский

Development of a CRISPR/Cas12a-facilitated fluorescent aptasensor for sensitive detection of small molecules

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 280, P. 136041 - 136041

Published: Sept. 26, 2024

Language: Английский