Effective Identification and Highly Sensitive Quantification of Fructo-oligosaccharide Isomers with Bi2Se3 Nanosheet-Assisted Laser Desorption Ionization Mass Spectrometry DOI
Ruochen Guo, Rui Lv,

Tianrong Yu

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(43), P. 24082 - 24092

Published: Oct. 15, 2024

The growing interest in fructo-oligosaccharides (FOSs) necessitates the effective monitoring of product quality. Identifying and quantifying FOS isomers from same sources are challenging. Here, we report a new method using Bi2Se3 nanosheets as matrix for matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS), achieving differentiation oligosaccharide through MALDI-MS/MS. Notably, four pentasaccharides two heptasaccharides were successfully identified, with remarkably low limit detection 0.06 pmol. Our approach enabled specific quantification 1F-fructofuranosylnystose commercial products, positioning it promising tool isomer nutritional food products. Furthermore, this technique facilitates rapid sensitive various saccharides wide range other small molecules enhanced signal intensities improved reproducibility. Overall, rapid, selective, molecules, enhancing analytical chemistry science applications.

Language: Английский

The Impact of the Mass Analyzer and Tissue Section Thickness on Spatial N-Glycomics with MALDI-MSI DOI
Dušan Veličković, Marija Veličković,

Christopher L. O’Connor

et al.

Journal of the American Society for Mass Spectrometry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

We compared matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) spatial N-glycomics data sets from Fourier-transform ion cyclotron resonance (FTICR) and orthogonal accelerated time-of-flight (timsTOF) spectrometers of FFPE preserved human kidney samples. also tested different tissue section thicknesses. In these analyses, we assessed the impact analyzer thickness on N-glycan coverage, sensitivity, histological alignment. Our results indicate negligible differences in coverage between two analyzers, where annotation numbers remained consistent were highly reproducible. The timsTOF-MS analyses demonstrated significant advantages with higher duty cycles better lateral resolution, allowing for finer resolution without compromising signal integrity. Specifically, timsTOF was able to generate detailed MALDI-MS images at 20 μm step size, accurately identifying Hex:5 HexNAc:5 dHex:1 as a tubular-specific marker observable delocalization. Despite minor discrepancies, only three species detected by FTICR not using timsTOF, few false-positive annotations analysis attributed lower resolving power, overall consistency instruments high. Importantly, did affect sensitivity average glycan intensity remaining stable 7 2 sections. These findings demonstrate that thick slices can be effectively used workflows, maintaining while enhancing confidence pathohistological evaluations.

Language: Английский

Citations

1
Recent advances in analytical methods and bioinformatic tools for quantitative glycomics DOI

Ji-Hong Lu,

Shuhong Guo,

Qiannan Liu

et al.

Analytical and Bioanalytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

Language: Английский

Citations

0
Detection of Retinoic Acid in Cosmetics Using Reactive Paper Spray Ionization Mass Spectrometry DOI Creative Commons

Yuzhang Bao,

Chenyu Wang, Na Zhang

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(9), P. 1906 - 1906

Published: April 25, 2025

Chromatography–mass spectrometry typically requires a time-consuming and costly pretreatment to detect illegal additives in cosmetics. Retinoic acid is classified as prohibited additive cosmetics by the European Union China. Therefore, rapid convenient method needed for its detection. In this study, detecting retinoic using Reactive Paper Spray Ionization Mass Spectrometry was developed. N′-dimethylpiperazine used derivatization reagent due ability react with carboxyl functional groups at room temperature. Our results indicate that derivatized compounds obtained exhibited good linearity within range of 0.0005~0.1 μg·mL−1, achieving limit detection 0.107 ng·mL−1.

Language: Английский

Citations

0
Mini Review: Highlight of Recent Advances and Applications of MALDI Mass Spectrometry Imaging in 2024 DOI Open Access

Yuen T. Ngai,

David C.W. Lau, Parul Mittal

et al.

Analytical Science Advances, Journal Year: 2025, Volume and Issue: 6(1)

Published: May 10, 2025

Abstract Matrix‐assisted laser desorption/ionisation mass spectrometry imaging (MALDI‐MSI) is an emerging tool that allows visualisation of hundreds analytes unbiasedly in a single experiment. This paper highlights the adaptations MALDI‐MSI different context 2024, such as clinical diagnostic, pharmacology, forensics applications, plant metabolism and biology. Challenges advancements were also discussed regarding sample preparation, instrumentations, data analysis, integration machine learning trend cell resolution multi‐omics. There are still rooms for improvements sensitivity, spatial resolution, acquisition algorithm across multi‐omics to enable at subcellular level.

Language: Английский

Citations

0
Effective Identification and Highly Sensitive Quantification of Fructo-oligosaccharide Isomers with Bi2Se3 Nanosheet-Assisted Laser Desorption Ionization Mass Spectrometry DOI
Ruochen Guo, Rui Lv,

Tianrong Yu

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(43), P. 24082 - 24092

Published: Oct. 15, 2024

The growing interest in fructo-oligosaccharides (FOSs) necessitates the effective monitoring of product quality. Identifying and quantifying FOS isomers from same sources are challenging. Here, we report a new method using Bi2Se3 nanosheets as matrix for matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS), achieving differentiation oligosaccharide through MALDI-MS/MS. Notably, four pentasaccharides two heptasaccharides were successfully identified, with remarkably low limit detection 0.06 pmol. Our approach enabled specific quantification 1F-fructofuranosylnystose commercial products, positioning it promising tool isomer nutritional food products. Furthermore, this technique facilitates rapid sensitive various saccharides wide range other small molecules enhanced signal intensities improved reproducibility. Overall, rapid, selective, molecules, enhancing analytical chemistry science applications.

Language: Английский

Citations

1