Wavelength-Resolved Magnetic Multiplex Biosensor for Simultaneous and Ultrasensitive Detection of Pneumonia Pathogens via Catalytic Hairpin Assembly Strategy with Luminescent Iridium Complexes

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

Pneumonia is a prevalent acute respiratory infection and major cause of mortality hospitalization, the urgent demand for rapid, direct, highly accurate diagnostic method capable detecting both Streptococcus pneumoniae (S. pneumoniae) Klebsiella (K. arises from their prominent roles as primary pathogens responsible pneumonia. Herein, two luminescent iridium complexes with nonoverlapping photoluminescence spectra, iridium(III)-bis [4,6-(difluorophenyl)-pyridinato-N,C2′] picolinate (abbreviated Ir–B) bis (2-(3,5- dimethylphenyl) quinoline-C2,N′) (acetylacetonato) iridium(III)) Ir-R), were unprecedently proposed to construct novel wavelength-resolved magnetic multiplex biosensor simultaneous detection S. K. based on catalytic hairpin assembly (CHA) signal amplification strategy combined dye-doped silica nanoparticles. Notably, not only exhibits broad linear range 50 pM 10 nM but also demonstrates excellent recovery rates (96.1–99.3%) (94.8–101.5%) in real clinical samples, corresponding relative standard deviation (RSD) values ranging 2.57 3.15% 1.45 3.17% pneumoniae. These favorable experimental outcomes undoubtedly offer promising approach multiple future.

Language: Английский

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The Trend of Nonenzymatic Nucleic Acid Amplification: Strategies and Diagnostic Application

Precision Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Language: Английский

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Controlled activation of a DNA walker driven by mismatched catalytic hairpin assembly for microRNA imaging in cancer cells and clinical samples

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: 437, P. 137747 - 137747

Published: April 10, 2025

Language: Английский

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Zinc-Organic Gel with Self-Catalysis-Enhanced Electrochemiluminescence as an Emitter for the Evaluation of Liver Cancer Markers

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

Herein, a novel zinc-organic gel with self-catalysis-enhanced electrochemiluminescence (ECL) performance was prepared as an emitter for the first time to assemble biosensor ultrasensitive detection of microRNA-221 (miR-221) related liver cancer. Interestingly, Zn

Language: Английский

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DNA Nanotechnology for Application in Targeted Protein Degradation

ACS Biomaterials Science & Engineering, Journal Year: 2024, Volume and Issue: 10(11), P. 6814 - 6827

Published: Oct. 5, 2024

DNA is a kind of flexible and versatile biomaterial for constructing nanostructures nanodevices. Due to high biocompatibility programmability easy modification fabrication, nanotechnology has emerged as powerful tool application in intracellular targeted protein degradation. In this review, we summarize the recent advances design mechanism degradation technologies such hydrolysis chimeras, lysosomal autophagy based Subsequently, introduce nanotechnologies cascade circuits, nanostructures, dynamic machines. Moreover, present latest developments Finally, vision challenges are discussed.

Language: Английский

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Target-assisted self-cleavage DNAzyme electrochemical biosensor for MicroRNA detection with signal amplification

Chemical Communications, Journal Year: 2024, Volume and Issue: 60(88), P. 12904 - 12907

Published: Jan. 1, 2024

In this work, we reported an electrochemical biosensor with target-assisted self-cleavage DNAzyme function for signal amplified detection of miRNA.

Language: Английский

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