Programmable DNA‐Based Nanodevices for Next‐Generation Clinical and Healthcare Applications

Advanced Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

Abstract Deoxyribonucleic acid (DNA) nanotechnology has brought an unparalleled set of possibilities for self‐assembled structures emerging as independent branch synthetic biology. The field science uses the molecular properties DNA to build nanoparticles and nanodevices that have potential bring breakthroughs in medical science. On one hand, their biocompatibility, precision, ease, programmability make them ideal choice drug delivery healthcare. other, lack proper biodistribution profiles, stability inside system, enzymatic cleavage, immune recognition, translational barriers are some hurdles it faces. Many recent technological advancements progress tackle these challenges, while already been used. These tools technologies need be understood studied successful transition intelligent nanostructures (DNs) healthcare applications. This review thus, highlights challenges being faced by DNs Additionally, provides overview trends using devices disease detection remission finally talks about future scope opportunities effective from bench bedside.

Language: Английский

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Endogenous Enzyme-Activated Spatial Confinement DNA Nanowire with a Tumor Cell-Specific Response for High-Precision Imaging of the Tumor/Normal Cells Boundary

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 12, 2025

Developing tumor cell-specific imaging approaches is essential for the clear delineation of margins. However, traditional suffered from low reaction kinetics as well limited specificity resulting their "always active" sensing mode, making it difficult to accurately depict boundary. To address these limitations, we developed an endogenous enzyme-activated spatial confinement DNA nanowire probe (E-SCNW) with enhanced tumor/normal cell discrimination ratio high precision cells The effect can improve kinetics, and enzyme-activation design confine fluorescence response region. Additionally, no additional delivery carriers were required during cross membrane into intracellular space. It worth noting that benefiting design, detection limit was decreased by nearly 25.6-fold 4.46-fold through using E-SCNW, indicating promising prospects in high-precision

Language: Английский

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On‐Site Multiply Stimulated Self‐Confinement of an Integrated DNA Cascade Circuit for Highly Reliable Intracellular Imaging of miRNA and In Situ Interrogation of the Relevant Regulatory Pathway

Small, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 16, 2024

Abstract Artificial DNA circuits represent a versatile yet promising toolbox for in situ monitoring and concomitant regulation of diverse biological events within live cells. Nonetheless, their performance is significantly impeded by the diffusion‐dominated slow reaction kinetics uncontrollable off‐target activation. Herein, self‐localized cascade (SLC) circuit reported robust efficient microRNA (miRNA) analysis living The SLC consists cell‐specific localization module analyte‐specific signal amplification module. By integrating probes these two modules, complexity system reduced to realize responsive co‐localization circuitry simultaneous amplification. Taking advantage specifically activated, self‐localized, design, successfully achieves miRNA‐21 (miR‐21) imaging accurate cells differentiation. Moreover, reverse mechanism explored between messenger RNA (mRNA) miRNA through engineered further elucidates underlying signaling pathways them. Therefore, provides powerful tool sensitive detection intracellular biomolecules study corresponding cell regulatory mechanisms.

Language: Английский

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Dual mature microRNA-responsive logic biosensing platform based on CRISPR/Cas12a and DNA nanocage

Talanta, Journal Year: 2024, Volume and Issue: 283, P. 127078 - 127078

Published: Oct. 23, 2024

Language: Английский

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