Lectin-Mediated Labeling of Alkaline Phosphatase for Enzymatic Silver Deposition-Based Electrochemical Detection of Glycoprotein Tumor Markers

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

The screening of glycoprotein markers has become an integral part the in vitro diagnosis malignant tumors. Herein, electrochemical method based on alkaline phosphatase (ALP)-mediated enzymatic silver deposition is reported for highly sensitive detection tumor markers, which ALP enzymes are decorated to glycan moieties targets via lectin-carbohydrate interactions. As glycoproteins conjugated with multiple chains, lectin-mediated labeling can result decoration each target enzymes. Moreover, hydrolysis ascorbic acid 2-phosphate into a high density particles, then be sensitively assayed robust Ag/AgCl solid-state voltammetric process. result, deposition-based exhibits sensitivity. Using aptamer-based breast cancer-associated CA15-3 as proof concept, limit 0.32 mU/mL been demonstrated. Results show that synergism capture and glycoform-specific discrimination capability interactions endow this selectivity, its practical use assay levels serum samples illustrated. With benefits from efficiency, mild reaction conditions, user-friendly operation, applicable low-abundance markers.

Language: Английский

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Wearable MOF Biosensors: A New Frontier in Real-Time Health Monitoring

TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 118156 - 118156

Published: Jan. 1, 2025

Language: Английский

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Research Progress of Electrochemical Biosensors for Diseases Detection in China: A Review

Biosensors, Journal Year: 2025, Volume and Issue: 15(4), P. 231 - 231

Published: April 5, 2025

Disease diagnosis is not only related to individual health but also a crucial part of public prevention. Electrochemical biosensors combine the high sensitivity electrochemical methods with inherent selectivity biological components, offering advantages such as excellent sensitivity, fast response time, and low cost. The generated electrical signals have linear relationship target analyte, allowing for identification concentration detection. This has become very attractive technology. review offers summary recent advancements in biosensor research disease China. It systematically categorizes summarizes developed China detecting cancer, infectious diseases, inflammation, neurodegenerative disorders. Additionally, delves into fundamental working principles, classifications, materials, preparation techniques, other critical aspects biosensors. Finally, it addresses key challenges impeding advancement examines promising future directions their development.

Language: Английский

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Dual-Screen-Printed Electrode Platform for Simultaneous Detection of IgG and IgM Antibodies using Magnetic Beads Nanocomplexes

Analytical Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 115864 - 115864

Published: April 1, 2025

Language: Английский

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Sandwich-like electrochemical immunosensing of IL-17 based on silver nanoparticles/graphdiyne and methylene blue/UiO-66-NH2

Microchimica Acta, Journal Year: 2025, Volume and Issue: 192(5)

Published: April 17, 2025

Language: Английский

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Advanced electrochemical detection methodologies for assessing neuroactive substance variability induced by environmental pollutants exposure

Environmental Technology & Innovation, Journal Year: 2024, Volume and Issue: unknown, P. 103965 - 103965

Published: Dec. 1, 2024

Language: Английский

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Surface-Functionalizing Strategies for Multiplexed Molecular Biosensing: Developments Powered by Advancements in Nanotechnologies

Nanomaterials, Journal Year: 2024, Volume and Issue: 14(24), P. 2014 - 2014

Published: Dec. 14, 2024

Multiplexed biosensing methods for simultaneously detecting multiple biomolecules are important investigating biological mechanisms associated with physiological processes, developing applications in life sciences, and conducting medical tests. The development of biosensors, especially those advanced biosensors multiplexing potentials, strongly depends on advancements nanotechnologies, including the nano-coating thin films, micro–nano 3D structures, nanotags signal generation. Surface functionalization is a critical process applications, one which enables immobilization probes or other structures that assist capturing biomolecules. During this functionalizing process, nanomaterials can either be objects surface modification materials used to modify base surfaces. These surface-functionalizing strategies, involving coordination sensor materials, as well modifying methods, largely determinative performance applications. This review introduces current studies potentials focuses specifically roles design these biosensors. A detailed description paradigms method selection has been set forth understanding accelerate application novel nanotechnologies

Language: Английский

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Ratiometric Electrochemical Detection of Interleukin-6 Using Electropolymerized Methylene Blue and a Multi-Walled Carbon-Nanotube-Modified Screen-Printed Carbon Electrode

Biosensors, Journal Year: 2024, Volume and Issue: 14(10), P. 457 - 457

Published: Sept. 25, 2024

Herein, we report a ratio-based electrochemical biosensor for the detection of interleukin-6 (IL-6). We electropolymerized methylene blue (MB) on surface screen-printed carbon electrodes; introduced an internal reference signal probe; modified carboxylate multi-walled nanotubes electrode to increase electrochemically active area; and finally linked amino-modified IL-6 aptamer through Schiff base reaction, with bovine serum albumin (BSA) added mask non-specific adsorption. After adding samples, I

Language: Английский

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A flexible multiplexed electrochemical biosensing platform with graphene and gold nanoparticle modification for enhanced e-ELISA point-of-care biomarker detection

Microchemical Journal, Journal Year: 2024, Volume and Issue: unknown, P. 112437 - 112437

Published: Dec. 1, 2024

Language: Английский

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