Cited by Nicking Enzyme Assisted Amplification Combined with CRISPR-Cas12a System for One-Pot Sensitive Detection of APE1

Space assembly of Ag nanoclusters on a metal-organic framework enhanced electrochemiluminescence for human epididymis protein 4 analysis DOI

Dongyu Han,

Xinyu Liu,

Feng Jiang

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: 209, P. 112675 - 112675

Published: Jan. 4, 2025

Language: Английский

Citations

A Primer-Regulated Rolling Circle Amplification (RCA) for Logic-Controlled Multiplexed Enzyme Analysis DOI

Shuiqin Chai, Weimin Sun, Xin Hou

et al.

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

DNA-related enzymes are associated with various diseases and have been potential biomarkers for clinical diagnosis. Developing robust ultrasensitive methods is extremely favorable the detection of these biomarkers. To this purpose, a primer-regulated rolling circle amplification (RCA) strategy was ingeniously proposed. Briefly, RCA primer, which invalidated 3'-inverted dT (locked state) unable to initiate an reaction by phi29 DNA polymerase, embedded recognition substrate specific enzyme. In presence target, cleavage process enzyme prompted release regeneration 3'-OH (unlocked state), satisfying vital prerequisite RCA. By adopting programmable modular design, can be either single base sites or sequence different types enzymes. This also enables us conduct multiple conveniently, relying on logic-controlled manner including YES, OR, AND, AND-OR operations. Overall, proposed uniquely insightful provides universal tool analyses diverse

Language: Английский

Citations

Nicking Enzyme Assisted Amplification Combined with CRISPR-Cas12a System for One-Pot Sensitive Detection of APE1 DOI

Wei Dai,

Han Wang,

Hanxu Ji

et al.

The Analyst, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Apurinic/apyrimidinic endonuclease 1 (APE1) is a critical enzyme in the base excision repair (BER) pathway, essential for preserving cellular equilibrium. Variations APE1 activity within blood or tissues can provide significant insights clinical cancer screening and disease diagnosis. Consequently, detection of diagnostics. However, there currently deficiency rapid, straightforward, sensitive methods detection. To address this issue, we developed method that integrates Nicking Enzyme Assisted Amplification (NEAA) with CRISPR-Cas12a signal amplification, enabling one-pot activity. This utilizes NEAA to produce substantial quantity target DNA complementary crRNA, thereby triggering trans-cleavage Cas12a. The activated Cas12a then amplifies emits signals by cleaving reporter probe. Our strategy allows swift precise APE1, only 3 h, threshold × 10-6 U mL-1 linear range 5 0.1 mL-1. It has been effectively utilized biological samples.

Language: Английский

Citations

PAMAM-Based DNA Fluorescence Nanoprobe for Rapid Whole Cellular APE1 Detection and Imaging DOI

Huimin Li,

Xinru Yuan,

Mei Ren

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 19, 2025

Apurinic/apyrimidinic endonuclease 1/redox effector factor 1 (APE1/ref-1, APE1), a vital protein for DNA repair and cellular redox regulation, is frequently overexpressed in tumor cells, underscoring the importance of developing sensitive detection methods early cancer diagnosis. However, rapid visualization nuclear APE1 cells are still challenging. In this study, we successfully developed novel fluorescent nanoprobe based on polyamidoamine (PAMAM) cytoplasmic APE1. The PAMAM surface was modified with arginine (Arg), named PR, its hydrophobic core encapsulated 1,6,7,12-tetrachloroperylene tetracarboxylic acid dianhydride (TA) dye to construct nanoparticles (TPR). Furthermore, an APE1-responsive dsDNA (SP) linked TPR surface, containing apurinic/apyrimidinic sites (AP sites) black hole quencher 2 (BHQ2) ensuring that fluorescence remains off absence TPR-SP exhibited range 0.125-25 U mL-1 limit as low 0.03 mL-1. Compared Arg-free nanoprobes (TP-SP), significantly accelerated endocytosis penetration, reducing time one-quarter (from 0.5 h). Notably, signal whole nucleus can also be detected. Thus, achieves enrichment amplification signals, leading highly detection. This innovative efficient method greatly expands technological means

Language: Английский

Citations

An enzyme-free multi-stage hybridization chain reaction for the electrochemiluminescence detection of MRSA using MoS2 NF@AuNPs catalyst DOI

Yudie Sun,

Kehong Quan,

Rong Zhang

et al.

Food Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 144043 - 144043

Published: March 1, 2025

Language: Английский

Citations

Nanoconfinement‐Enhanced Aggregation‐Induced Electrochemiluminescence for Smartphone‐Adopted Imaging Analysis of cTnI DOI

Chuanping Li, Zhen Yang, Jing Wang

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Abstract The domain‐limited catalytic enhancement has opened novel avenues for the advancement of highly efficient aggregation‐induced electrochemiluminescence (AIECL). Herein, a (ECL) sensing platform is constructed through in situ encapsulation emission (AIE) molecules, tetraphenylethylene, within metal‐organic frameworks (NH 2 ‐MIL‐88). These are uniquely enriched with atomically dispersed active sites that serve as nanoconfined co‐reactant accelerators. This innovative design leads to creation an integrated AIE nanoconfinement reactor. Within this reactor, accelerators catalyze co‐reactants into reactive species (RS) situ, facilitating direct interaction molecules spatially confined composite. Density functional theory calculations and electrochemical electron paramagnetic resonance demonstrate ECL attributed localized amplification, where space formed by coordination self‐assembly alters activation energy barriers improves absorption capacity catalyzing K S O 8 SO 4 ·‐ OH ·− . Additionally, utilizing MATLAB‐mediated image technology deep learning algorithms, smartphone‐adopted self‐reporting AIECL imaging system designed achieve accurate intelligent analysis cardiac troponin I (cTnI). method presents advanced strategy enhance local RS concentrations via catalysis. developed offers facile screening cTnI monitoring.

Language: Английский

Citations

PER-Based DNA Cube Captor for High Specific and Efficient SERS Detection of HPV DOI

Y. Liu,

Huan Liang, Ruo Yuan

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 7, 2025

Early detection of human papillomavirus (HPV) is crucial for the treatment and prevention some specific skin diseases related cancers such as cervical cancer, anal penile etc. Herein, we designed a primer exchange reaction (PER)-based DNA cube captor (PDCC) to capture Raman molecular labeled high specificity efficient HPV. First, converted target into large amounts single-stranded based on PER. Second, initiated catalytic hairpin assembly (CHA) reaction, facilitating onto nanostructures (DCNs). Then DCNs were subsequently immobilized gold hexagonal plates (GHPs) detection. Of note, identification by PER exhibits specificity, occurs exclusively when perfectly matches hairpin. Moreover, CHA can occur four sides this structure, efficiency enhanced proximity effect. This work developed device named PDCC that captured DNA, which realize highly sensitive HPV-58. has HPV, plays an indispensable role in diagnosis cancers.

Language: Английский

Citations

Double Self‐Amplified Programmable Allosteric DNA Nanomachine for Enzymatically Triggered Spatially Controllable Molecular Imaging DOI

Minghui Hu, Yingyu Zhang, Jie Zhang

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: May 15, 2025

Abstract In vivo optical tumor molecular imaging encounters significant challenges in achieving adequate specificity and sensitivity, largely attributed to off‐tumor signal leakage the relatively low expression levels of target molecules. Therefore, a double self‐amplified programmable allosteric DNA nanomachine (named HPs‐tFNA) is developed through two elaborately designed hairpin structures (HP1 HP2) hybridized on tetrahedral framework (tFNA), enabling rapid, specific, sensitive using highly specific apurinic/apyrimidinic endonuclease 1 (APE1) cytoplasm as stimulus‐response target. presence APE1, HP2 modifies sites (AP sites), which can be specifically recognized cleaved by releasing number cyclic sequences (cyclic‐seq) initial APE1‐assisted amplification. Subsequently, cyclic‐seq hybridizes with HP1, inducing conformational change that converts stem‐loop structure HP1 linear form. This structural facilitates spatial separation fluorophore quencher, thereby generating fluorescence signals. Furthermore, APE1 incises AP within loop region, resulting release cyclic‐seq. The released hybridize additional continuously amplify manner, second round amplification assisted APE1. experimental results this study demonstrated HPs‐tFNA achieve rapid situ guide precise surgical excision vivo, superior specificity. particular, effectively monitor drug resistance neuroblastoma cells stratify risk via plasma analysis.

Language: Английский

Citations

Ternary Heterojunction ZnS‐ZnIn₂S₄‐In₂S₃ Efficiently Catalyzes Triethylamine to Enhance Electrochemiluminescence Imaging Signals DOI

Jingui Chen, Yue Jia, Huaiqing Zhao

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 20, 2024

Abstract The visualization of electrode reactions is significant for ultra sensitive detection. Traditional electrochemiluminescence (ECL) detection method can hardly visualize reactions. Combining ECL imaging technology and traditional methods overcome the lack visual feedback key information in sensor construction. In contrast to cyclic voltammetry testing, applying a constant voltage surface results more stable signal. this study, methionine‐stabilized gold nanoclusters (Met‐AuNCs) are used as efficient near‐infrared luminescent clusters, triethylamine (TEA) coreactants construct an acetamiprid (ACT). ternary heterojunction ZnS‐ZnIn 2 S 4 ‐In 3 (Z‐Z‐I) forms stepwise electron transfer mode, facilitating rapid generation free radicals TEA. Furthermore, Z‐Z‐I serves substrate, providing large loading area DNA chain, which lays foundation wide range. Such has linear range (100 f m 1 µ ) achieves low limit (42 fM) ACT This study not only provides accurate detecting agricultural products but also foretells promising application electrochemiluminescent sensors.

Language: Английский

Citations

Sequentially Activated-Dumbbell DNA Nanodevices for Accurate Detection of Uracil-DNA Glycosylase via PER-Based Orthogonal Signal Outputs DOI

Xiaoqing Yuan, Yan‐Mei Lei,

Ying-Huan Li

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(42), P. 17013 - 17020

Published: Oct. 11, 2024

Accurate and reliable detection of uracil-DNA glycosylase (UDG) activity is crucial for clinical diagnosis prognosis assessment. However, current techniques accurately monitoring UDG still face significant challenges due to the single input or output signal modes. Here, we develop a sequentially activated-dumbbell DNA nanodevice (SEAD) that enables precise evaluation through primer exchange reactions (PER)-based orthogonal output. The SEAD incorporates double-hairpin structure with stem containing two deoxyuridine (dU) sites target recognition preblocked binding regions amplification Upon dU, can be cleaved by apurinic/apyrimidinic endonuclease 1 (APE1), generating different hairpins exposed regions. These serve as templates initiate parallel PER, enabling extending products: long single-stranded (ssDNA) repetitive sequences short ferrocene-labeled ssDNA complementary sequences. products further self-assemble into nano-strings in an manner act electrochemiluminescence switch, low-abundance UDG. This work develops sequential strategy activity, highlighting potential cancer treatment.

Language: Английский

Citations