Functionalized pNIPAM-DNA Hydrogel Colorimetric Platform for Visual Detection of Low-Mass Soluble β-Amyloid Oligomers

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 7, 2025

Low-mass soluble β-amyloid oligomers (LSAβO) are critical Alzheimer's disease (AD) biomarkers with significant diagnostic and therapeutic potential. However, their application in early screening detection is limited by the reliance on complex analytical instruments procedures. To address this, we developed a visual sensing platform for LSAβO using functionalized pNIPAM-DNA hydrogel. Exploiting temperature-responsive nature of pNIPAM, hydrogel selectively incorporates enriches from solution via temperature-induced expansion contraction. binding to aptamers triggers formation G-quadruplex DNAzymes, which catalyze oxidation 3,3',5,5'-tetramethylbenzidine generate visible colorimetric signal. The hydrogel's small pore size further enhances selectivity excluding larger during real sample analysis. This sensor exhibits linear range 0.1-7.5 nM limit 50 pM. Combining enrichment exclusion, this provides cost-effective, highly sensitive, selective, high-throughput approach preliminary detection.

Language: Английский

Proximity hybridization-triggered cascade amplification for label-free SERS detection of Alzheimer's amyloid-β oligomers

The Analyst, Journal Year: 2024, Volume and Issue: 150(2), P. 264 - 271

Published: Dec. 17, 2024

Most of the existing SERS systems failed to achieve satisfactory results in early diagnosis Alzheimer's disease owing a lack effective signal transduction.

Language: Английский

Advanced electrochemical detection methodologies for assessing neuroactive substance variability induced by environmental pollutants exposure

Environmental Technology & Innovation, Journal Year: 2024, Volume and Issue: unknown, P. 103965 - 103965

Published: Dec. 1, 2024

Language: Английский