Toehold and Hairpin Assembly-Mediated Tripedal DNA Walker for Circulating Tumor DNA Detection in Human Blood DOI
Wei Li, Jiayue Li,

Shuaijing Wang

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 424, P. 136887 - 136887

Published: Nov. 1, 2024

Language: Английский

An Ultrasensitive and Specific Fluorescence Split-Aptasensor for D-Vp Detection Based on Target-Induced Self-Propelled 3d-Dna Walkers Coupled with Crispr-Cas12a DOI
Xiang Li, Yuting Zhang, Mengyuan He

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
DNA Nanoframework-Confined Well-Organized Catalytic Hairpin Assembly for Highly Sensitive and Specific Detection of MiRNA DOI

Yongbin Hou,

Yong Huang,

Yan-e Du

et al.

Talanta, Journal Year: 2025, Volume and Issue: 290, P. 127788 - 127788

Published: Feb. 21, 2025

Language: Английский

Citations

0
RCA-Supported Multipedal DNA Walker Integrated with TSDR for Simultaneous Detection of Single Nucleotide Polymorphisms in Circulating Tumor DNA DOI
Wei Li,

Jiayue Li,

Minghong Lin

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

Peripheral blood circulating tumor DNA (ctDNA) is a crucial liquid biopsy biomarker that correlates overall systemic burden with malignant progression. However, identifying multiple single nucleotide polymorphisms (SNPs) in ctDNA presents significant challenges. In this study, we developed rolling circle amplification (RCA)-supported multipedal walker integrated toehold-mediated strand displacement (TSDR) to facilitate the detection of SNPs. Initially, separate identification ctDNAs performed using TSDR procedures, along release triggers for RCA. Once RCA processes are initiated, long single-stranded oligonucleotide products containing repeated DNAzyme sequences generated. Finally, these function as legs traverse track quasi-mechanical manner, producing detectable fluorescence signals. The walking mechanism exhibits high efficiency and signal capability, can interact probes simultaneously. Concurrent wild type (WT) mutation (MT) achieved, limits at attomolar (aM) level, linear range 10 aM 5.0 μM. Furthermore, proposed method demonstrates remarkable specificity mismatched single-base mutant genes, due TSDR-assisted process. Additionally, analysis SNPs clinical serum samples reveals differences expression between cancer patients healthy individuals. Therefore, be employed potential early diagnosis, treatment monitoring, prognosis.

Language: Английский

Citations

0
An ultrasensitive and specific fluorescence split-aptasensor for D-VP detection based on target-induced self-propelled 3D DNA walkers coupled with CRISPR-Cas12a DOI
Xiang Li, Yuting Zhang, Mengyuan He

et al.

Talanta, Journal Year: 2025, Volume and Issue: 293, P. 128102 - 128102

Published: April 8, 2025

Language: Английский

Citations

0
Controlled activation of a DNA walker driven by mismatched catalytic hairpin assembly for microRNA imaging in cancer cells and clinical samples DOI
Liuting Mo,

Shiya Tang,

Fenghua Meng

et al.

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: 437, P. 137747 - 137747

Published: April 10, 2025

Language: Английский

Citations

0
Steric Engineering of Perylene Derivative Side Chains To Boost Electrochemiluminescence for High-Performance Bioanalysis DOI
Fang Yang,

Xinya Jiang,

Chao Tan

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 20, 2025

Traditional organic planar aromatic emitters often encounter low electrochemiluminescence (ECL) efficiency due to the aggregation-induced quenching (ACQ) induced by π-π stacking, limiting their applicability in bioanalysis. In this work, we proposed a steric engineering strategy mitigate ACQ perylene diimide-bridged β-cyclodextrin aggregates (Pe-CD aggregates), significantly boosting ECL for trace microRNA (miRNA) detection. By incorporation of large volume cyclic (β-CD) at both side chains diimide, Pe-CD molecules were generated and further assembled into robust supramolecular nanoblocks. This modulates intermolecular distances around cores, effectively minimizing nonradiative relaxation. Molecular simulation results demonstrated that distance increased from 3.32 Å 5.21 Å, accompanied 5.8-fold enhancement compared Pe (formed dense stacking molecules). As proof concept, an bioassay platform was developed using as target-enrichment accelerated-3D DNAzyme walkers signal amplifiers detecting miRNA-21, achieving limit detection 9.7 aM. study offers valuable insights designing new expands potential applications technology.

Language: Английский

Citations

0
A transformable DNA nanomachine serving as both walker and track for sensitive miRNA detection in living cells and tissues DOI
Liuting Mo, Rongzheng Yuan,

Shiya Tang

et al.

Talanta, Journal Year: 2025, Volume and Issue: 295, P. 128300 - 128300

Published: May 8, 2025

Language: Английский

Citations

0
Stochastic bipedal dual-DNA walkers for fast and sensitive detection of apurinic/apyrimidinic endonuclease1 and inhibitor screening DOI

Qingyi Liu,

Qiongdan Zhang,

Yu Mao

et al.

Talanta, Journal Year: 2024, Volume and Issue: 280, P. 126758 - 126758

Published: Aug. 24, 2024

Language: Английский

Citations

1
Enzyme-Assisted Fluorescence Biosensor Based on Circular Single-Stranded DNA Without Group Modification for MicroRNA Detection DOI Creative Commons

Xiaoxue Yin,

Yazhen Liao,

Feiyu Li

et al.

Biosensors, Journal Year: 2024, Volume and Issue: 14(11), P. 527 - 527

Published: Nov. 1, 2024

Fluorescent biosensor, which has the characteristics of high sensitivity, specificity, and low cost, can be directly detected in physiological fluids such as blood serum. Therefore, development fluorescence sensor platforms for miRNA detection a positive effect on prevention treatment various diseases. In this paper, miR-34a was selected biological indicator Alzheimer's disease (AD). We designed circular single-stranded DNA (CSSD) uses two unmodified (ssDNA) with complementary ends, DNAa DNAb, to form CSSD by sequence pairing improve thermal stability achieve signal amplification. At same time, react miR-34a, then DNA-RNA chain is hydrolyzed duplex-specific nuclease (DSN enzyme). Finally, released partake subsequent step, thus realizing cycle By evaluating change under optimized conditions, we discovered that approach exhibits impressive threshold reaching 0.36 nM. This surpasses performance numerous preceding biosensors. Furthermore, system displays excellent capabilities even intricate settings like serum, showcasing strong ability differentiate choose effectively. summary, signal-off fluorescent realizes purpose double amplification biosensor using enzyme assistance so it used valuable tool early diagnosis

Language: Английский

Citations

1
Toehold and Hairpin Assembly-Mediated Tripedal DNA Walker for Circulating Tumor DNA Detection in Human Blood DOI
Wei Li, Jiayue Li,

Shuaijing Wang

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 424, P. 136887 - 136887

Published: Nov. 1, 2024

Language: Английский

Citations

0